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EC number: 801-277-8 | CAS number: 507448-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- only one scrubber used
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
- EC Number:
- 801-277-8
- Cas Number:
- 507448-65-9
- Molecular formula:
- C7H10F3NO2
- IUPAC Name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on August 23, 1999.
The pH after collection was 7.2.
The preparation was carried out according to the method described in the guideline.
The concentration of the inoculum in the test was 25.3 mg sludge/L.
- Details on study design:
VESSELS:
2 L flasks (dark brown glass) equipped with gas inlet and magnetic stirrer.
RECONSTITUTED WATER:
The reconstituted water was prepared according to the method described in the guideline.
TEMPERATURE:
20 ± 2°C
DURATION:
29 days
AIR:
≡ 25 ml/min purified from carbon dioxide.
CO2 –SCRUBBERS:
Approx. 250 ml scrubbers with gas inlet tubes equipped with sintered glass tips containing 250 ml 0.05N sodium hydroxide.
DEVIATION:
Only one CO2 scrubber was used per test bottle. Theoretically, a solution of 0.05N NaOH is sufficient to trap at least two times more CO2 than the maximum ThCO2 which can evolve in each test bottle (including CO2 possibly evolved from the bacteria e.g., endogenous respiration). Moreover, experimentally it was confirmed that no measurable CO2 carry over has ever occurred with the scrubbers used.
FLASKS:
Flasks 1 and 2: test medium and inoculum (inoculum blank)
Flask 3 and 4: reference substance and inoculum (procedure control)
Flask 5 and 6: test item and inoculum
Flask 7: reference item, test item and inoculum (control of toxicity and inhibition of the bacteria’s activity by the tests substance)
Flask 8: dist. Water
TEST CONCENTRATION:
Test item: Replicate 1: 52.4 mg test item/1.5L = 22.3 mg ThOC/L
Replicate 2: 52.9 mg test item/1.5L = 22.6 mg ThOC/L
Reference substance: 15 mg DOC/L
For the toxicity control, where the test item and reference were applied together, the reference amount was 15.0mg DOC/L and the test item was 22.5 mg ThOC/L.
Before application, the inoculum was pre-acclimated to the test medium over-night. The aeration was performed inside 1.2L water, containing the amount of mineral salts for 1.5L.
The final volume was made up to 1.5L with 300 ml mixture made from the test item (the above mentioned amount) in bidistilled water. This mixture was homogenized by ultrasonification for 5 minutes and added to the test vessel, which was immediately connected to the CO2 scrubber.
DURATION AND SAMPLING:
The test duration was 29 days. During this time the evolved CO2 trapped by the 0.05 N NaOH solution of the blank and test item was measured at 3, 6, 8, 10, 13, 16, 20, 24, 28 and 29 days.
The last measurement was performed after acidification of the test medium wit 1 ml of concentrated hydrochloric acid.
ANALYTICAL DEVICE:
At each sampling day the CO2 trapped in the scrubbers was measured as inorganic carbon with a carbon analyser (Shimadzu TOC-5050 A).
CALCULATIONS/STATISTICAL ANALYSIS:
The biodegradation was calculated on the basis of the theoretical carbon content of the test item and the quantities of inorganic carbon determined on the days of measurements in the absorbers
Results and discussion
- Test performance:
- Test validity:
The cumulative amount of CO2 in the blank flasks were 37.7 and 37.4 mg/1.5L, equivalent to 10.3 and 10.2 mg TOC/1.5L.
The total CO2 evolution in the inoculum blank at the end of the test did not exceed 60 mg/1.5.
These results show that the test system was working properly and no CO2 leaks were present.
The biodegradation of the reference substance reached the level for ready biodegradablility in a 10-day window. After 10 days on incubation 91 % of the reference compound sodium benzoate, had degraded, there the test is valid.
- Details on results:
The percentage biodegradation, based on CO2 evolution versus time for CA 2455 A and the reference substance (± CA2455 A) – Refer to Tables 1 and 2.
The theoretical calculated amount of CO2 in the test flasks with the test item alone were 81.7 and 82.8 mg/1.5L, equivalent to 22.3 and 22.6 mg TOC/1.5L. The actual found amount of TOC/1.5L at the end of exposure were -4.1 and 2.6 mg C (C measured in the test vessels minus mean C content measured in the blanks) equivalent to 15.0 and 9.5 mg/1.5L of CO2 in the test flasks with the test item alone.
The high rate of CO2 evolution in the toxicity control (reference + test item), indicates that the test item did not inhibite the biodegradation of the reference substance.
The biodegradation of the test item at the end of the test was 18 and 12 %, respectively (Tables 1 and 2). Therefore the degradation of the test item was:
15 % in 29 days.
Pass level (60 %): none
Time window: none
The mineralisation of CA 2219 A under these test conditions was 1% in 29 days. Based on the results of this test alone and using the classification range given in Annex VI of directive 92/69/EEC C.4-C, the ecotoxicological classification is
“not readily biodegradable”.
BOD5 / COD results
- Results with reference substance:
- Refer to details on results.
Any other information on results incl. tables
Table 1: CO2 evolution and biodegradation of the controls; together Blank, reference substance and reference + test item.
CO2 evolution measured as mg C |
Biodegradation |
||||||||
|
Blank |
Reference |
Reference + test Substance |
Reference |
Reference + Test Substance |
||||
|
1 |
2 |
mean |
1 |
2 |
mean |
|
|
|
Day |
mg |
mg |
mg |
mg |
mg |
mg |
mg |
% |
% * |
0 |
0.00 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0 |
0 |
3 |
3.7 |
3.7 |
3.7 |
16.8 |
16.6 |
16.7 |
15.6 |
58 |
53 |
6 |
5.8 |
5.6 |
5.7 |
23.9 |
21.0 |
22.5 |
22.5 |
75 |
75 |
8 |
6.4 |
6.4 |
6.4 |
26.7 |
25.5 |
26.1 |
24.8 |
87 |
81 |
10 |
7.0 |
6.8 |
6.9 |
27.7 |
27.0 |
27.3 |
25.9 |
91 |
84 |
13 |
7.8 |
7.6 |
7.7 |
29.4 |
29.4 |
29.4 |
28.2 |
96 |
91 |
16 |
8.5 |
8.2 |
8.3 |
31.1 |
31.0 |
31.1 |
30.5 |
101 |
99 |
20 |
9.1 |
8.8 |
8.9 |
32.3 |
32.1 |
32.2 |
32.0 |
104 |
103 |
24 |
9.9 |
9.3 |
9.6 |
33.2 |
33.0 |
33.1 |
33.1 |
105 |
105 |
28 |
10.2 |
9.8 |
10.0 |
33.8 |
33.7 |
33.8 |
34.4 |
105 |
108 |
29 |
10.3 |
10.2 |
10.2 |
34.3 |
34.3 |
34.3 |
34.5 |
107 |
108 |
*The biodegradation of the ‘reference + test item’ control is calculated with the applied amount of the reference alone, ie., 15 mg DOC/L
Table 2: CO2 evolution and biodegradation of the test item.
Day |
CO2 evolution Measured as mg C |
Biodegradation Of test substance |
|||
1 |
2 |
1 |
2 |
mean |
|
0 |
0.0 |
0.0 |
0 |
0 |
0 |
3 |
3.8 |
4.1 |
1 |
2 |
1 |
6 |
7.2 |
7.5 |
7 |
8 |
8 |
8 |
9.1 |
8.7 |
12 |
10 |
11 |
10 |
10.0 |
9.6 |
14 |
12 |
13 |
13 |
11.0 |
10.3 |
15 |
11 |
13 |
16 |
11.9 |
11.1 |
16 |
12 |
14 |
20 |
12.6 |
10.3 |
17 |
6 |
11 |
24 |
13.4 |
11.1 |
17 |
7 |
12 |
28 |
13.7 |
12.9 |
16 |
13 |
14 |
29 |
14.3 |
12.8 |
18 |
12 |
15 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The mineralisation of CA 2455 A under these test conditions was 15% in 29 days.
Based on the results of this test alone and using the classification range given in Annex VI of directive 92/69/EEC C.4-C, the ecotoxicological classification is
“not readily biodegradable”. - Executive summary:
Novartis Crop Protection AG, Product Portfolio Management, Environmental Safety, Ecotoxicology, CH-4002 Basel, Switzerland.
A measured volume (1.5 L) of inoculated mineral medium containing 52.4 mg resp. 52.9 mg test item
(22.3 resp. 22.6 mg TOC/1.5 L) as the nominal sole source of organic carbon is aerated by the passage of carbon dioxide-free air at a controlled rate in diffuse light. Degradation is followed over 29 days by determining the carbon dioxide produced, which is trapped in sodium hydroxide and which is measured as inorganic carbon by a carbon analyzer. The amount of carbon dioxide produced from the test chemical (corrected for that derived from the blank inoculum) is expressed as a percentage of theoretical carbon dioxide.
A reference and a reference together with the test item as toxicity control were used as controls.
The inoculum concentration in the test bottle was 25.3 mg suspended solids/L.
The pH of the sludge after collection was 7.2.
In the test with CA 2455 A tech. the temperature was maintained constant at 21 ± 1 °C.
The biodegradability of the reference substance after 10 days was 91 %.
The biodegradation calculated as percentage of measured amount of inorganic carbon over the theory was:
15 % in 29 days
Pass level (60 %): none
Time window: none
Conclusion:
The mineralisation of CA 2455 A under these test conditions was 15% in 29 days.
Based on the results of this test alone and using the classification range given in Annex VI of directive 92/69/EEC C.4-C, the ecotoxicological classification is
“not readily biodegradable”.
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