Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-756-6 | CAS number: 87-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: According to Guideline. Not conducted under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-chloro-o-toluidine
- EC Number:
- 201-756-6
- EC Name:
- 3-chloro-o-toluidine
- Cas Number:
- 87-60-5
- Molecular formula:
- C7H8ClN
- IUPAC Name:
- 3-chloro-2-methylaniline
- Details on test material:
- - Test substance number: 85/48
- name of test substance: 3-chlor-2-methylanilin
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Gaukler: D-6065 Offenbach/Main, FRG
- Mean weight at study initiation: 3.02 kg (male), 3.20 kg (females)
- Housing: single
- Diet: Kliba 341, 4 MM; Klingentalmuehle AG, CH-4303 Kaiseraugst, Switzerland; approx. 130 g/animal/day
- Water: approx. 250 mL tap water per animal per day
- Acclimation period: at least 8 days before testing
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the untreated eye served as control
- Amount / concentration applied:
- 0.1 mL of the unchanged test item
- Duration of treatment / exposure:
- 15 days, since no wash out was performed.
- Observation period (in vivo):
- 15 days
reading time points: 1 h, 24 h, 48 h, 72 h, 8 days and 15 days after application - Number of animals or in vitro replicates:
- 3 (1 male; 2 females)
- Details on study design:
- SCORING SYSTEM: according to Draize, as recommended by the OECD 405
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- iris score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: reversibility: not applicable
- Remarks on result:
- other: individual scores: 0 - 0 - 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 15 days
- Remarks on result:
- other: individual scores: 1 - 1 - 1
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8 days
- Remarks on result:
- other: individual scores: 1 - 1 - 1
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 1.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8 days
- Remarks on result:
- other: individual scores: 2 - 2 - 1
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 1.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8 days
- Remarks on result:
- other: individual scores: 2 - 1 - 1
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 15 days
- Remarks on result:
- other: individual scores: 2 - 2 - 2
Any other information on results incl. tables
In animal #1, loss of corneal tissue was noted 1 h after application. Suppuration was observed in animal #2 24 h after instillation. On day 15, loss of hair was noted for animal #1. Marginal vascularization of the cornea was seen in animal #1 and 2 on day 8 and in animal #2 on day 15. Moreover, small retractions in the eyelids were noted in all animals an day 8 and in animals #2 and 3 on day 15. As outlined in the raw data, slight opacity covered the complete corneal surface in all animals throughout the study period. The reversibility of these finding till day 21 is doubtful.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the irreversible signs of corneal opacity in all rabbits, 3-chloro-o-toluidine has to be classified for eye irritation R41 or Category 1 according to EEC/67/548 and Regulation (EU) No. 1272/2008, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.