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reaction product of: saturated, monounsaturated and multiple unsaturated long-chained partly estrified alcohols of vegetable origin (Brassica napus L., Brassica rapa L., Helianthus annuus L., Glycine hispida, Gossypium hirsutum L., Cocos nucifera L., Elaeis guineensis) with O,O-diisobutyldithiophosphate and 2-ethylhexylamine and hydrogen peroxide
EC number: 428-630-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 17,1998 to January 20,1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted in compliance with agreed test protocols following guidance.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 428-630-5
- EC Name:
- -
- Molecular formula:
- Not applicable
- IUPAC Name:
- reaction product of Z-9-octadecen-1-ol and O,O-diisobutyl hydrogen dithiophosphate
- Details on test material:
- - Substance type: orange-yellow, clear liquid
- Physical state: orange-yellow, clear liquid
- Lot/batch No.: 12447
- Storage condition of test material: in a well closed container and protected from light at room temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
none
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The nominal concentration of the test group was 100 mg/l. 100 mg test substance and 1 1 test medium were stirred for 24 hours and centrifbged at 3,500 rpm for 10 minutes in order to separate the unsolved substance. The clear aqueous phase was used for the test. The amount of the test substance dissolved in test medium was checked by DOC analysis (Dissolved Organic Carbon) at the beginning of the test and after 72 hours.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The nominal concentration of the test group was 100 mg/l. 100 mg test substance and 1 1 test medium were stirred for 24 hours and centrifbged at 3,500 rpm for 10 minutes in order to separate the unsolved substance. The clear aqueous phase was used for the test.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: algae
- Source (laboratory, culture collection): SAG - Sammlung von Algenkulturen, University Giittingen
- Age of inoculum (at test initiation): no data
- Method of cultivation: The stock culture growth up in conical flasks containing nutrient solution. The algae were shaken permanently and were incubated at 22-24 oC with continuous lighting.n.
- Culturing media and conditions:
Culture medium:
NH4Cl 15.0 mg/l
MgCl2.6H2O 12.0 mg/l
CaCl2.2H2O 18.0 mg/l
MgSO4.7H2O 15.0 mg/l
KH2PO4 1.6 mg/l
FeCl3.6H2O 0.08 mg/l
Na2EDTA.2H2O 0.10 mg/l
H3BO3 0.185 mg/l
MnCl2.4H2O 0.415 mg/l
ZnCl2 0.003 mg/l
CoCl2.6H2O 0.0015 mg/l
CuCl2.2H2O 0.00001 mg/l
Na2MoO4.2H2O 0.007 mg/l
NaHCO3 50.0 mg/l
- Any deformed or abnormal cells observed: no data
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none.
Test conditions
- Hardness:
- no data
- Test temperature:
- 22.5-23.4 oC
- pH:
- 6.9 - 8.4
- Dissolved oxygen:
- no data
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentratins: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks, 100 ml
- Test volume: 30 ml
- Aeration: yes
- Initial cells density (test): 1.0 x 10^4 cells/mL
- Initial cells density (control): 1.00 x 10^4 cells/mL
- Control end cells density: 34.3x 10^4 cells/mL
GROWTH MEDIUM
- Standard medium used: no
- Detailed composition if non-standard medium was used:
NH4Cl 15.0 mg/l
MgCl2.6H2O 12.0 mg/l
CaCl2.2H2O 18.0 mg/l
MgSO4.7H2O 15.0 mg/l
KH2PO4 1.6 mg/l
FeCl3.6H2O 0.08 mg/l
Na2EDTA.2H2O 0.10 mg/l
H3BO3 0.185 mg/l
MnCl2.4H2O 0.415 mg/l
ZnCl2 0.003 mg/l
CoCl2.6H2O 0.0015 mg/l
CuCl2.2H2O 0.00001 mg/l
Na2MoO4.2H2O 0.007 mg/l
NaHCO3 50.0 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous
- Salinity (for marine algae): not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: limit test - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- none
- Results with reference substance (positive control):
- not applicable.
- Reported statistics and error estimates:
- none.
Any other information on results incl. tables
Determination of the Cell Concentration
The cell concentration in each flask was determined at 24, 48 and 72 hours after start of the test by counting the cells in a counting chamber. Four samples of each test vessel were counted. The average values were calculated. Results are shown in table 1.
Table1:Average values of cell concentration of the replicates at24,48and72hours after start of test
nominal concentration of test substance (mg/l) |
24hours x104cells/ml |
48hours x104cells/m |
72hours x104cells/m |
0 |
4.86 |
21.38 |
34.27 |
100 |
4.69 |
20.11 |
33.29 |
At the nomind concentration of100 mg/l test substanceno inhibition of cell growth was determined.
The cell concentrationinthe control cultures should have increased by a factor of at least16within three days. This condition was fulfilled.
Determination of the Concentration of the Test Substance
The amount of the test substance dissolved in test medium was checked by DOC analysis (Dissolved Organic Carbon) at the beginning of the test and after 72 hours. The result of analysis was compared with the carbon content of the test substance of 61.9 per cent (Table 2).
Table 2: DOC concentration during the test
|
concentration ofDOC (correctedfor theDOCcontent in the control) mg/l |
calculated concentration of test substance mg/l |
0 hours |
2.87 |
4.6 |
72hours |
2.57 |
4.2 |
The concentration of test substance in test water, measured as DOC, was 4.6 mg/l at the start of the test. After 72 hours the concentration was 4.2 mg/l.
Conclusion
The IC50 (72 hours) alga of the test substance is greater than 100 mg/l (nominal concentration).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (72 hours) alga of the test substance is greater than 100 mg/l (nominal concentration).
- Executive summary:
Methods:
A study was performed to assess the effect of the test material on the growth of the algaScenedesmus subspicatus. The method followed that described in Method C.3 of Commission Directive 92/69/EEC.
Procedure:
The nominal concentration of the test group was 100 mg/l. 100 mg test substance and 1L test medium were stirred for 24 hours and centrifuged at 3,500 rpm for 10 minutes in order to separate the unsolved substance. The clear aqueous phase was used for the test. Stock culture of algae was added to get an initial cell concentration of 10^4 cell/ml.
Results:
Determination of the Cell Concentration
The cell concentration in each flask was determined at 24, 48 and 72 hours after start of the test by counting the cells in a counting chamber. Four samples of each test vessel were counted. The average values were calculated. Results are shown in table 1.
Table1:Average values of cell concentration of the replicates at24,48and72hours after start of test
nominal concentration
of test substance (mg/l)
24hours
x104cells/ml
48hours
x104cells/m
72hours
x104cells/m
0
4.86
21.38
34.27
100
4.69
20.11
33.29
At the nominal concentration of100 mg/l test substanceno inhibition of cell growth was determined.
The cell concentrationinthe control cultures should have increased by a factor of at least16within three days. This condition was fulfilled.
Determination of the Concentration of the Test Substance
The amount of the test substance dissolved in test medium was checked by DOC analysis (Dissolved Organic Carbon) at the beginning of the test and after 72 hours. The result of analysis was compared with the carbon content of the test substance of 61.9 per cent (Table 2).
Table 2: DOC concentration during the test
concentration ofDOC
(correctedfor theDOCcontent in the control)
mg/l
calculated concentration of test substance
mg/l
0 hours
2.87
4.6
72hours
2.57
4.2
The concentration of test substance in test water, measured as DOC, was 4.6 mg/l at the start of the test. After 72 hours the concentration was 4.2 mg/l.
Conclusion
The IC50 (72 hours) alga of the test substance is greater than 100 mg/l (nominal concentration).
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