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EC number: 211-367-3 | CAS number: 640-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well performed GLP and OECD guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Manganese Oxalate, dihydrate
- IUPAC Name:
- Manganese Oxalate, dihydrate
Constituent 1
Test system
- Amount / concentration applied:
- Approximately 10 mg of the neat test item were applied to each of triplicate EPISKIN (Skinethic) tissues. In order to improve contact and to guaranty a uniform and complete coverage of the epidermis, the test material was wetted with 15 µL deionised water.
- Duration of treatment / exposure:
- The skin equivalents were exposed to the test item for 15 minutes. Afterwards the test items was washed off and the equivalents were incubated for further 42 hours.
- Details on study design:
- Approximately 10 mg of the neat test item were applied to each of triplicate EPISKIN (Skinethic) tissues. In order to improve contact and to guaranty a uniform and complete coverage of the epidermis, the test material was wetted with 15 µL deionised water.
The test item and the positive and negative controls were washed off the skin tissues after 15 minutes treatment. After further incubation for about 42 hours the tissues were treated with the MTT solution for 3 hours following approximately 69 hours extraction of the colorant from the cells. The amount of extracted colorant was determined photometrically at 570 nm.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- mean of 3 runs
- Value:
- 92.2
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Other effects / acceptance of results:
- After treatment with the test item Manganese Oxalate Dihydrate the relative absorbance values decreased slightly to 92.2%. This value is well above the threshold for irritancy of ≤ 50%. Therefore, the test item is not considered to possess an irritant potential.
Any other information on results incl. tables
Results after treatment with Manganese Oxalate Dihydrate
Dose group |
Treat-ment Interval |
Absor-bance 570 nm |
Absor-bance 570 nm |
Absor-bance 570 nm |
Mean Absor-bance of 3 Tissues |
Relative Absor-bance [%] Tissue 1, 2, 3** |
Stan-dard De-viation [%] |
Rel. Absor-bance [% of Negative Control]*** |
Negative Control |
15 min |
1.079 |
0.873 |
1.021 |
0.991 |
108.9 |
10.7 |
100.0 |
Positive Control |
15 min |
0.390 |
0.324 |
0.208 |
0.308 |
39.4 |
9.3 |
31.0 |
Test Item |
15 min |
0.778 |
1.015 |
0.948 |
0.914 |
78.5 |
12.3 |
92.2 |
* Mean of two replicate wells after blank correction
** relative absorbance per tissue [rounded values]: (100 x (absorbance tissue )) / (mean absorbance negetive control)
*** relative absorbance per treatment group [rounded values]: (100 x (mean absorbancetest item)) / (mean absorbancenegetive control)
Optical evaluation of the MTT-reducing capacity of the test item after 1 hour incubation with MTT-reagent did not show blue colour.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item Manganese Oxalate Dihydrate is non irritant to skin.
- Executive summary:
This in vitro study was performed to assess the irritation potential of Manganese Oxalate Dihydrate by means of the Human Skin Model Test.
Three tissues of the human skin model EpiSkin™ were treated with the test item, the negative or the positive control for 15 minutes.
Approximately 10 mg of the neat test item were applied to each of triplicate tissues. In order to improve contact and to guaranty a uniform and complete coverage of the epidermis, the test material was wetted with 15 µL deionised water.
The test item and the positive and negative controls were washed off the skin tissues after 15 minutes treatment. After further incubation for about 42 hours the tissues were treated with the MTT solution for 3 hours following approximately 69 hours extraction of the colorant from the cells. The amount of extracted colorant was determined photometrically at 570 nm.
10 µL of either the negative control (deionised water) or the positive control (5% Sodium lauryl sulfate) were applied to each tissue.
After treatment with the negative control the absorbance values were well within the required acceptability criterion of mean OD ≥0.6 till ≤1.5 for the15 minutes treatment interval thus showing the quality of the tissues.
Treatment with the positive control induced a decrease in the relative absorbance as compared to the negative control to 31.0% thus ensuring the validity of the test system.
The standard deviations between the % variabilities of the test item, the positive and negative controls were below 13% (threshold of the "OECD Guideline for the Testing of Chemicals 439: In vitro Skin Irritation: Reconstructed Human Epidermis Test Method”: 18%), thus ensuring the validity of the study.
After treatment with the test item Manganese Oxalate Dihydrate the relative absorbance values decreased slightly to 92.2%. This value is well above the threshold for irritancy of≤50%. Therefore, the test item is not considered to possess an irritant potential.
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