Registration Dossier
Registration Dossier
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EC number: 231-659-4 | CAS number: 7681-11-0
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- genetic toxicity in vivo, other
- Remarks:
- Drosophila lethal mutation test
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Gene mutation in vivo toxicity study of the test chemical
- Author:
- Law
- Year:
- 1 938
- Bibliographic source:
- Proceedings of the National Academy of Sciences of the United States of America
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- In vivo Drosophila lethal mutation test was performed to determine the mutagenic nature of the test chemical
- GLP compliance:
- not specified
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- Potassium iodide
- EC Number:
- 231-659-4
- EC Name:
- Potassium iodide
- Cas Number:
- 7681-11-0
- Molecular formula:
- IK
- IUPAC Name:
- potassium iodide
- Details on test material:
- - Name of test material: potassium iodide
- Molecular formula : KI
- Molecular weight : 166.0 g/mol
- Substance type: Inorganic
- Physical state: Solid
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Oregon-R stock
- Details on species / strain selection:
- No data
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: No data
- Assigned to test groups randomly: No data
- Fasting period before study: No data
- Housing: The flies were raised in ½ pint milk bottles
- Diet (e.g. ad libitum): Standard food of corn meal, molasses and agar, with brewers' yeast added,
- Water (e.g. ad libitum): No data
- Acclimation period: No data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data
IN-LIFE DATES: From: To: No data
Administration / exposure
- Route of administration:
- other: Micro-injection
- Vehicle:
- No data
- Details on exposure:
- No data
- Duration of treatment / exposure:
- No data
- Frequency of treatment:
- No data
- Post exposure period:
- No data
Doses / concentrations
- Remarks:
- 0.5-1.0 cubic mm
- No. of animals per sex per dose:
- 6 males
- Control animals:
- not specified
- Positive control(s):
- No data
Examinations
- Tissues and cell types examined:
- X-chromosome
- Details of tissue and slide preparation:
- Other:
The lethals were detected by the usual C1B method:
(1) C1B/x-ple* female by + (treated) male
(2) F1 of C1B/+ female by x-ple male
(3) Females from cultures indicating lethal or semi-lethal mutations were mated by x-ple males. - Evaluation criteria:
- No data
- Statistics:
- No data
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutagenic potential
- Additional information on results:
- No data
Any other information on results incl. tables
Table: Effect of various test chemical on the lethal mutation rate in the x-chromosome of the Oregon-R race of Drosophila Melanogater
Chemical |
Treatment |
Time |
No. of animals |
No, of chromosomes tested |
No. of lethals |
% lethals |
Location of lethals |
Mutation rate |
Test chemical |
Injection |
- |
6 |
386 |
2 |
0.52 |
- |
- |
Applicant's summary and conclusion
- Conclusions:
- The test chemical did not induce lethal mutations in X- chromosomes of Drosophila Oregon-R stock and is hence it is not likely to be mutagenic in vivo.
- Executive summary:
Drosophila lethal mutation assay was performed to determine the mutagenic nature of the test chemical in vivo. The study was performed using male Oregon-R stock Drosophila flies. The test chemical was injected into larvae by use of a micro-injectionfollowing the method of Beadle and Ephrussi.As per this method, 0.5-1.0 cubic mm od substance may be injected into each larva. The gonads were thus bathed in the solution. The treatment after injection of 0.038 per cent I2 in 0.075 per cent KI did not produce a significant increase in the lethal mutation rate of the X-chromosome. Based on the observations made, thetest chemical did not induce lethal mutations in X- chromosomes of Drosophila Oregon-R stock and is hence it is not likely to be mutagenic in vivo.
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