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EC number: 202-681-1 | CAS number: 98-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 5 to 24 June 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The test item was tested in the chromosome aberration assay using Chinese hamstar ovary cells, with and without metabolic activation. The treated cells were harvested at 14 hours and 24 hours following the initiation of chemical treatment and scored for chromosome aberration
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 4-chloro-α,α,α-trifluorotoluene
- EC Number:
- 202-681-1
- EC Name:
- 4-chloro-α,α,α-trifluorotoluene
- Cas Number:
- 98-56-6
- Molecular formula:
- C7H4ClF3
- IUPAC Name:
- 1-chloro-4-(trifluoromethyl)benzene
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): compound 38502
- Storage condition of test material: room temperature
- Other: received on May 26, 1983
The test article was measured and dissolved in DMSO to appropriate concentrations, immediatly before use. Approximately 10 to 20 minutes were taken between the time the test article was sulubilized and the final treatment of cells.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- - Originally obtained from American Type Culture Collection, Rockville, Maryland
- Properly maintained: yes (cryopreserved) - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 containing 2.4 mg/ml of NADP, 4.5 mg/ml of isocitric acid and 15 µl of Aroclor induced rat liver homogenate/ml.
- Test concentrations with justification for top dose:
- The test article was tested at six decreasing dose levels from 130.0 nl/ml to 29.99 nl/ml in the activated and non-activated systems.
- Vehicle / solvent:
- Solvent: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- triethylenemelamine
- cyclophosphamide
- Details on test system and experimental conditions:
- DURATION
Cells were treated with test substance without S9 for 12 hours, with S9 for 2 hours.
NUMBER OF CELLS EVALUATED: 100
DETERMINATION OF CYTOTOXICITY
- Method: rate of surival cells - Statistics:
- Percentage of cells with aberration.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The relative cell survival (RCS): 0% at 200 nl/ml, RCS 87% at 60 nl/ml in activated system - 0% at 200 nl/ml and 103 at 60 nl/ml in the nonactivated system.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: CHO-K1
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Test article concentration | Number of cells analyzed | Number of aberrations per cell | % of cells with aberration |
130.00 nl/ml | Cells were dead | - | - |
110.07 nl/ml | Cells were dead | - | - |
90.25 nl/ml | 100 | 0 | 0 |
70.40 nl/ml | 100 | 0.02 | 2 |
49.98 nl/ml | 100 | 0.03 | 2 |
29.98 nl/ml | 100 | 0.04 | 4 |
Negative control | 100 | 0.01 | 1 |
Positive control 50 µg/ml | 100 | 1.07 | 59 |
Solvent control | 100 | 0.01 | 1 |
Results of chromosome aberration assay-14 hours without activation
Test article concentration | Number of cells analyzed | Number of aberrations per cells | % of cells with aberration |
80.00 nl/ml | Cells were dead | - | - |
70.00 nl/ml | 100 | 0.02 | 2 |
59.97 nl/ml | 100 | 0.02 | 2 |
49.97 nl/ml | 100 | 0.01 | 1 |
39.98 nl/ml | 100 | 0.04 | 4 |
29.98 nl/ml | 100 | 0.03 | 2 |
Negative control | 100 | 0.04 | 3 |
Positive control 1.0 µg/ml | 100 | 1.34 | 65 |
Solvent control | 100 | 0.06 | 4 |
Results of chromosome aberration assay-24 hours with activation
Test article concentration | Number of cells analyzed | Number of aberrations per cell | % of cells with aberration |
130.00 nl/ml | Cells were dead | - | - |
110.07 nl/ml | Cells were dead | - | - |
90.25 nl/ml | 100 | 0.01 | 1 |
70.40 nl/ml | 100 | 0.05 | 4 |
49.98 nl/ml | 100 | 0.03 | 3 |
29.98 nl/ml | 100 | 0.05 | 5 |
Negative control | 100 | 0.04 | 4 |
Positive control 50 µg/ml | 100 | 2.98 | 81 |
Solvent control | 100 | 0.03 | 3 |
Results of chormosome aberration assay-24 hours without activation
Test article concentration | Number of cells analyzed | Number of aberrations per cell | % of cells with aberration |
80.00 nl/ml | Cells were dead | - | - |
70.00 nl/ml | 100 | 0.02 | 2 |
59.97 nl/ml | 100 | 0 | 0 |
49.97 nl/ml | 100 | 0.01 | 1 |
39.98 nl/ml | 100 | 0.01 | 1 |
29.98 nl/ml | 100 | 0.01 | 1 |
Negative control | 100 | 0.02 | 2 |
Positive control 1.0 µg/ml | 100 | 1.17 | 47 |
Solvent control | 100 | 0.03 | 3 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Compared to the cells with chromosome aberrations in the solvent control, none of the test doses showed a significant increase in the frequency of cells with chromosome aberrations in the activated and nonactivated system.
In this study no indication of a dose response was observed.
In this study the positive controls caused a significant increase in the number of cells with chromosome aberrations. - Executive summary:
The test item was tested in the chromosome aberration assay using Chinese hamstar ovary cells, with and without metabolic activation. The treated cells were harvested at 14 hours and 24 hours following the initiation of chemical treatment and scored for chromosome aberration. The results indicate that under the test condition, the test article did not cause a significant increase in the frequencies of chromosome abertration in the Chinese hamster ovary cells with and without rat S9 activation.
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