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EC number: 700-768-3 | CAS number: 1285610-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-10-21 - 2011-11-30
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: in-vitro skin irritation experiment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for Testing of Chemicals No. 431, April 13, 2004 (“In vitro Skin Corrosion: Human Skin Model Test”)
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for Testing of Chemicals No. 439, July 22, 2010 (“In vitro Skin Irritation: Reconstructed Human Epidermis Test Method”)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- BASF SE, Experimental Toxicology and Ecology
Test material
- Reference substance name:
- Bis-[N-C13-(branched and linear)-alykl]-ammonium O,O-dipropan-2-yl phosphorodithioate
- EC Number:
- 700-768-3
- Cas Number:
- 1285610-71-0
- Molecular formula:
- C26H56N.C6H14O2PS2
- IUPAC Name:
- Bis-[N-C13-(branched and linear)-alykl]-ammonium O,O-dipropan-2-yl phosphorodithioate
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- not applicable
Test system
- Amount / concentration applied:
- Single topical application of 50 μL (corrosion test) or 30 μL (irritation test) of the test substance to a reconstructed three dimensional human epidermis model (EpiDerm™).
- Duration of treatment / exposure:
- For the corrosion test two EpiDerm™ tissue samples were incubated with the test substance for 3 minutes and 1 hour, respectively. The irritation test was performed with three EpiDerm™ tissue samples, which were incubated with the test substance for 1 hour followed by an about 42-hours post-incubation period.
- Details on study design:
- - In the corrosion test, control tissues were concurrently applied with 50 μL of highly de-ionized water (negative control, NC) or with 50 μL of 8 n potassium hydroxide (positive control, PC) or test substance (killed tissue control, KC).
- In the irritation test, control tissues were concurrently applied with 30 μL of sterile PBS (negative control, NC) or with 30 μL of 5% SDS (positive control, PC). A nylon mesh was placed carefully onto the tissue surface afterwards.
- Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation using a colorimetric test. The reduction of mitochondrial dehydrogenase activity, measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the test substance treated epidermal tissues is compared to that of negative control tissues. The quotient of the values indicates the relative tissue viability.
- The OD570 values determined for the various tissues are measures of their viability. The quotient of the OD570 of tissues treated with the test material and the mean OD570 values of the NC (percent of control) is used for evaluating whether or not a test material is corrosive or irritant.
- If the reduction of MTT is influenced by the test substance (e.g. visible residues of the test substance on the tissues), subsequent testing of killed controls was considered. In case of direct reduction of MTT by the test substance, the OD570 values measured in the freeze-killed control tissues (KC) will be used to correct the mean OD570 of the testsubstance treated tissues (mean OD570 KC corrected).
Results and discussion
In vivo
- Other effects:
- The test substance is able to reduce MTT directly. Therefore an additional MTT reduction control was introduced (performed with the corrosion test, only).
Any other information on results incl. tables
Corrosion test:
The mean viability of the test-substance treated tissues determined after an exposure period of 3 minutes was 77%, and it was 84% after an exposure period of 1 hour. Residues of the test compound remained on the tissues after washing.
Irritation test:
The mean viability of the test-substance treated tissues determined after an exposure period of 1 hour with about 42 hours post-incubation was 12%. Visible residues of the test compound remained on the tissues after washing; however, due to the unequivocal result subsequent testing of MTT reduction control was not performed.
Table: Corrosion test:
Exposure: 3 min | Exposure: 1 hour | ||||||||
tissue 1 | tissue 2 | KC | mean | tissue 1 | tissue 2 | KC | mean | ||
negative control | mean OD570 | 1.803 | 1.803 | 0.229 | 1.803 | 1.791 | 1.815 | 0.166 | 1.803 |
viability [% of NC] | 100 | 100 | - | 100 | 99.3 | 100.7 | - | 100 | |
test material | mean OD570 | 1.561 | 1.606 | 0.425 | 1.387 | 1.667 | 1.663 | 0.323 | 1.509 |
viability [% of NC] | 86.6 | 89.1 | - | 77 | 92.5 | 92.2 | - | 84 | |
positive control | mean OD570 | 0.257 | 0.233 | - | 0.245 | 0.2 | 0.188 | - | 0.194 |
viability [% of NC] | 14.3 | 12.9 | - | 14 | 11.1 | 10.4 | - | 11 |
For the test substance the means are given after KC-correction.
KC = freeze-killed control tissues
Table: Irritation test:
tissue 1 | tissue 2 | tissue 3 | mean | SD | ||
negative control | mean OD570 | 1.719 | 1.928 | 1.741 | 1.796 | |
viability [% of NC] | 95.7 | 107.3 | 96.9 | 100 | 6.39 | |
test material | mean OD570 | 0.213 | 0.232 | 0.216 | 0.22 | |
viability [% of NC] | 11.8 | 12.9 | 12 | 12 | 0.57 | |
positive control | mean OD570 | 0.121 | 0.127 | 0.128 | 0.125 | |
viability [% of NC] | 6.7 | 7 | 7.1 | 7 | 0.21 |
Applicant's summary and conclusion
- Conclusions:
- Based on the observed results it was concluded, that the test article shows a skin irritation potential in the EpiDerm™ skin irritation test under the test conditions chosen.
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