(2S)-5-amino-2-[(aminoacetyl)amino]-5-oxopentanoic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

17 July 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Triplicate samples (3 x 2 x ~5 mL) were taken at the beginning and at the end of the renewal periods. Samples from the control were taken at the start and end of the renewal periods (1 x 2 x ~5 mL).
Sampled test item solutions were stored in a freezer until measurement.

Vehicle:

no

Details on test solutions:

A stock solution with a concentration of 100.0 mg anhydrous form of test item/L was prepared with direct addition of the test item, mixed into aquarium water using ultrasonic bath (approximately 30 seconds), then distributed into the appropriate test flasks. As a Limit test was carried out, further dilution of stock solution was not performed.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish (Brachydanio rerio)
- Source: Hungarian University of Agriculture and Life Sciences, Department of Aquaculture 2100 Gödöllő, Páter Károly u. 1., Hungary
- Length at study initiation (length definition, mean, range and SD): 1.7 – 2.3 cm
- Weight at study initiation (mean and range, SD): control: Measured weight
of 7 fish (g) => 1.14g; Calculated mean weight of 1 fish (g) => 0.16g; test item: Measured weight
of 7 fish (g) => 0.84g; Calculated mean weight of 1 fish (g) => 0.12g
- Method of breeding: Fish were bred in a standard commercial fish farm, under disease- and parasite-controlled conditions.

ACCLIMATION
- Acclimation period: >12d

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

171 mg CaCO3/L

Test temperature:

19.4 - 21.2°C

pH:

7.88 - 8.27

Dissolved oxygen:

80 - 100 %

Nominal and measured concentrations:

nominal: 100 mg/l
measured geometric mean: 91.2 mg/l

Details on test conditions:

TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): 24h renewal period
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.23 g fish/litre testing liquid in the control ; 0.17 g fish/litre testing liquid in the test item group

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light (artificial illumination) and 8 hours darkness

TEST CONCENTRATIONS
Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg L-Alanyl-L-tyrosine/l
- Results used to determine the conditions for the definitive study: yes => limit test

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 108.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

no

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this acute fish toxicity study on Zebrafish (Brachydanio rerio) the observed LC50 for the test item Glycyl-L-Glutamine monohydrate was > 108.9 mg/L (nominal) corresponding to a LC50 for the active ingredient Glycyl-L-Glutamine of > 100 mg/L (nominal).

Description of key information

LC50 (96 h): >100 mg/L (nominal, OECD203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L