Registration Dossier
Registration Dossier
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EC number: 831-109-9 | CAS number: 5837-73-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
In an immobilisation study according to OECD TG 202, EU Method C.2 and US EPA OCSPP 850.10 the 48-hour EC50 of the test item towards Daphnia magna was determined to be 2.84 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 2.84 mg/L
Additional information
To investigate the acute toxicity of the test item to aquatic invertebrates a semi-static immobilisation test with Daphnia magna according to OECD TG 202, EU Method C.2 and US EPA OCSPP 850.1010 was carried out in accordance with GLP principles. Twenty animals per treatment and control (divided in 4 replicates with 5 animals in each vessel) were exposed to nominal test item concentrations of 0.38, 0.75, 1.5, 3.0 and 6.0 mg/L. For determination of the test item concentrations, samples were taken from each concentration level and control at the start and at the end of each water renewal period, i.e. after 24 hours of exposure. Concentrations were determined by HPLC method with UV detection. The corresponding calculated exposure concentrations (based on analytical measurements) were the followings: 0.17, 0.44, 0.98, 2.09 and 4.20 mg/L. Biological results and endpoints are thus based on the geometric mean measured concentrations. An untreated control ran in parallel. In addition, a positive control with the reference compound Potassium dichromate was performed. The immobility or mortality of the Daphnia was determined by visual observation after 24 and 48 hours of exposure. To determine NOEC/LOEC values Fisher´s Exact Binomial Test with Bonferroni Correction, for determination of the ECx values Probit analysis was used. All validity criteria of the test guideline were met, therefore the study is considered to be fully valid. For the reference compound a 24h EC50 of 0.94 mg/L was determined, confirming the suitability of the applied test system. As a result, the 48 hour EC50 of the test item was 2.84 mg/L.
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