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EC number: 817-828-0 | CAS number: 80322-82-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 - 11 February 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[2-(2-hydroxyethoxy)ethoxy]ethanol,methanesulfonic acid
- Cas Number:
- 80322-82-3
- Molecular formula:
- C8H18O8S2
- IUPAC Name:
- 2-[2-(2-hydroxyethoxy)ethoxy]ethanol,methanesulfonic acid
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Oxidane
- Reference substance name:
- 1,2-Ethandiylbis(oxy-2,1-ethandiyl)-monomethanesulfonat
- IUPAC Name:
- 1,2-Ethandiylbis(oxy-2,1-ethandiyl)-monomethanesulfonat
- Test material form:
- liquid
- Details on test material:
- Appearance: Dark yellow liquid
Batch No.: 01110008
Storage: -5 – 40 °C, container tightly closed, protected from light
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- Batch no.: 01110008
Storage conditions: 2–8°C, protected from light
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from all test media at the start of the test as well as after 24 hours in the fresh and old media and after 48 hours of exposure. At the start of the test, the samples were taken from the freshly prepared single test solution as well as from the pure test water serving as control. For all subsequent sampling time points, aliquots from the respective replicates were pooled per treatment before sampling.
All samples were diluted with acetonitrile to obtain a composition of sample:acetonitrile (6:4; v:v). Subsequently, the samples were stored deep-frozen (at about -20 °C) until analysis. In non-GLP preliminary experiments, the test item proved to be stable under these storage conditions.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The following test vessels were set up:
- Test solution (Tn); containing test water with test item (four replicates with five Daphnia each)
- Blank control (Bn); containing pure test water (four replicates with five Daphnia each)
- Since the test item is soluble, the single test solution was prepared by adding 100 mg/l of test item to test water in glassware and stirring it for ten minutes until dissolution.
The test vessels were filled with 50 ml of the respective test medium. Daphnia, aged less than 24 hours and already acclimatised to the test water, were introduced into the test media and the vessels were covered with a glass plate. The Daphnia were not fed during the test and the test vessels were not aerated.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS
- Test organism: Daphnia magna Straus, supplied by IES Ltd., 4108 Witterswil, Switzerland
- Breeding: The daphnids are cultivated in Elendt M4 medium at 18–22 °C, controlled at ±1 °C
- Illumination: 16 h per day, about 550-1100 lux, source: overhead white fluorescent tubes
- Medium: Reconstituted water (Elendt M4 medium)
- Feed: Suspension of Desmodesmus subspicatus in reconstituted water with an optical density at 680 nm of about 12 units. This results in a final concentration of about 0.1 units from Monday to Thursday
and 0.4 units on Fridays in the culture vessel.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Hardness: 2.5 mM (based on the sum of Ca2+ and Mg2+), Carbonate Alkalinity: 0.8 mM
- Test temperature:
- 18–22 °C, controlled at ±1 °C
- pH:
- 7.5 to 7.8
- Dissolved oxygen:
- The minimum dissolved oxygen concentration in the controls and the test vessels was >=8.1 mg O2/l (required >=3 mg O2/l).
- Salinity:
- not reported
- Conductivity:
- not reported
- Nominal and measured concentrations:
- Since no significant effects were observed in the non-GLP range-finding test, the definitive test was performed as a limit-test at a nominal test item concentration of 100 mg/l.
The concentrations of Triethylene glycol dimethanesulfonate in the test media were determined by HPLC with UV detection in the samples from the test start as well as in the samples after 24 and 48 hours of exposure.
These analyses showed that the test item was correctly dosed and remained within 80–120% of the nominal concentration over the exposure period. Therefore, the effective concentrations ECx were assessed based on the nominal concentration of the test item. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml beakers, all-glass, with 50 ml of test medium, covered with a glass plate to avoid evaporation and contamination of the test solutions with dust
- Test medium: Elendt M4 medium; prepared with ultra-pure water (conductivity <1.5 µS/cm)
- Number of Daphnia: 20 individuals per test concentration, five per vessel
- Age: Less than 24 h
- Light: 16 hours photoperiod per day, about 550–1100 lux, supplied by overhead white fluorescent tubes
- Temperature: 18–22 °C, controlled at ±1 °C
- pH: 6 to 9. The pH should normally not vary by more than 1.5 units in one test.
- Feed: The Daphnia are not fed during the test
- Test type: Static exposure conditions
- Test duration: 48 h
RANGE-FINDING STUDY
Prior to the definitive test a non-GLP range finding test with nominal concentrations of 1, 10 and 100 mg/l of the test item was performed (two replicates for each of the test concentration and the control; five daphnids per replicate).
EFFECT PARAMETERS MEASURED
Observed immobility (inability to swim) of the daphnids: Observations of immobile Daphnia were made after 24 and 48 h of exposure. Any abnormal behaviour or appearance was reported. - Reference substance (positive control):
- yes
- Remarks:
- Acute reference test with potassium dichromate conducted twice a year.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- During the first 24 hours of the test, no immobilised test organisms were determined in the control and in the single test item concentration of 100 mg/l. Thus, the 24-hour EC50 was greater than 100 mg/l.
The no observed effect concentration (NOEC) after 24 hours was determined to be 100 mg/l.
After 48 hours of exposure, no immobilised test organisms were determined in the control and the single test item concentration of 100 mg/l. Thus, the 48-hour EC50 was greater than 100 mg/l.
The no observed effect concentration (NOEC) after 48 hours was determined to be 100 mg/l. - Results with reference substance (positive control):
- Acute ref. test with K2Cr2O7 conducted twice a year. The EC50 value for the control of sensitivity for 24 h of exposure with K2Cr2O7 was estimated to be 1.6 mg/l (September 2021), which lies within the recommended range of 0.6–2.1 mg/l acc. to OECD Guideline 202.
- Reported statistics and error estimates:
- The effective concentration EC50 for 24 and 48 hours was estimated based on the concentration tested and immobilisation observed.
The no observed effect concentration (NOEC) is the highest test concentration for which the test item is observed to have an immobilisation rate not exceeding 10%. Thus, immobilisation rates >10% are considered to be significant.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria i.e. immobilization in the controls <=10% and O2 concentrations at the end of the test >=3 mg/L were fulfilled.
- Conclusions:
- The acute toxicity of Triethylene glycol dimethanesulfonate (CAS no. 80322-82-3) to Daphnia magna was investigated according to the OECD guideline 202 under static exposure conditions over a period of 48 hours.
The median effect concentrations (EC50) of Triethylene glycol dimethanesulfonate on Daphnia magna were estimated to be >100 mg/l each nominal concentration after 24 and 48 h of exposure, respectively. The NOEC values were determined to be 100 mg/l each nominal concentration after 24 and 48 h of exposure, respectively. The results of the test can be considered reliable without restriction. - Executive summary:
The acute toxicity of Triethylene glycol dimethanesulfonate (CAS no. 80322-82-3) to Daphnia magna was investigated according to the OECD guideline 202 under static exposure conditions over a period of 48 hours.
The test item is liquid, 94.3% (area) pure and soluble in test water (Elendt M4 medium) up to at least 100 mg/l. The single nominal concentration tested was 100 mg/l.
The concentrations of Triethylene glycol dimethanesulfonate in the test media were determined by HPLC with evaporative light scattering detection in the samples from the test start as well as in the samples after 24 and 48 hours of exposure. These analyses showed that the single test concentration was correctly dosed and remained stable within 80–120% of the nominal value. Therefore, the effective concentrations ECx were assessed based on the nominal concentration of the test item.
In the control and at the test concentration of 100 mg/l, no immobilised test organisms were determined in the control and in the single test item concentration of 100 mg/l over the whole exposure period of 48 hours.
The biological test results of Triethylene glycol dimethanesulfonate to Daphnia magna after 24 and 48 hours of exposure are summarised in the following table based on nominal concentrations:Parameter Immobilisation (0-24 h)
[mg/l]
Immobilisation (24-48 h)
[mg/l]
EC50 >100 >100 NOEC 100 100 In conclusion, Triethylene glycol dimethanesulfonate (CAS no. 80322-82-3) had no acute toxic effects on Daphnia magna up to the nominal concentration of 100 mg/l under the conditions of the test.
All validity criteria were fulfilled.
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