Reaction products of diazotised sodium 2-amino-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate coupled with sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate, subsequently coupled with diazotised sodium 2-(4-aminobenzene-1-sulfonyl)ethyl sulfate

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

experimental test results performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

GLP compliance:

no

Type of method:

flask method

Partition coefficient type:

octanol-water

Analytical method:

titration method

Key result

Type:

log Pow

Partition coefficient:

-2.812

Temp.:

25 °C

Remarks on result:

other: other details are unknown

Table 1

 

Water phase
Ratio (n-octanol/water)	Concentration of test chemical (mg/L)		Mean concentration mg/L	Dilution factor
	C1	C2
1/2	524.664	524.771	524.718	10 times
1/1	1066.500	1066.840	1066.670	100 times
2/1	2221.670	2220.590	2221.130	100 times

 

Table 2

 

Octanol phase
Ratio(n-octanol/water)	Concentration of test chemical (mg/L)		Mean concentration mg/L	Dilution factor
	C1	C2
1/2	1.351	1.3722	1.362	2 times
1/1	1.666	1.6658	1.666	2 times
2/1	2.012	2.003	2.007	2 times

 

Table 3

 

Ratio(n-octanol/water)	C (n-octanol/water)	Pow	Log Pow	Mean of Log Pow±SD
1/2	1.3616/524.718	0.003	-2.586	-2.812±0.229
1/1	1.666/1066.670	0.002	-2.806
2/1	2.007/2221.130	0.001	-3.044

Conclusions:

The Partition coefficient (Pow) of the test chemical was determined to be -2.812±0.229 at 25 °C± 1°C.

Executive summary:

The partition Coefficient n-octanol/water of test chemical was determined by OECD Guideline No. 107, adopted on 27th July 1995,”Shake Flask Method”.

Two solvent were mutually pre-saturated at experiment temperature 25°C± 1°C. on the mechanical shaker, one containing n-Octanol with 0.280 mL of water and the other containing water with 0.470 mL of n-octanol for 100mL stock were shaken for 24 hours on a mechanical shaker. A stock solution was be prepared in n-Octanol (presaturated with water) by accurately weighing the test chemical which was introduced into 30 mL (1:1), 20 mL (1:2) and 40 mL (2:1) of pre-saturated n-Octanol respectively and mix with ratios of pre-saturated water. After separation, both the aqueous phase and n-Octanol phase were analyzed by Spectrophotometer.

 The partition coefficient was calculated, from the measured concentration of test chemical in the organic and aqueous phases. The log Pow value was determined to be -2.812±0.229 at 25°C± 1°C. Thus it can be concluded that the test chemical is hydrophilic in nature . 

Description of key information

The Partition coefficient (Pow) of the test chemical was determined to be -2.812±0.229 at 25 °C± 1°C.

Key value for chemical safety assessment

Log Kow (Log Pow):

-2.812

at the temperature of:

25 °C

Additional information

The partition Coefficient n-octanol/water of test chemical was determined by OECD Guideline No. 107, adopted on 27th July 1995,”Shake Flask Method”.

Two solvent were mutually pre-saturated at experiment temperature 25°C± 1°C. on the mechanical shaker, one containing n-Octanol with 0.280 mL of water and the other containing water with 0.470 mL of n-octanol for 100mL stock were shaken for 24 hours on a mechanical shaker. A stock solution was be prepared in n-Octanol (presaturated with water) by accurately weighing the test chemical which was introduced into 30 mL (1:1), 20 mL (1:2) and 40 mL (2:1) of pre-saturated n-Octanol respectively and mix with ratios of pre-saturated water. After separation, both the aqueous phase and n-Octanol phase were analyzed by Spectrophotometer.

 The partition coefficient was calculated, from the measured concentration of test chemical in the organic and aqueous phases. The log Pow value was determined to be -2.812±0.229 at 25°C± 1°C.Thus it can be concluded that the test chemical is hydrophilic in nature .