SDBR

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Storage temperature: at room temperature
Protected from light: no

Analytical monitoring:

yes

Details on sampling:

Samples of the only loading rate and the control were taken at t = 0h (newly prepared media) and t= 24h (spent media after 24h). Samples of exactly 100g were taken in 100mL brown glass bottles and transferred on the same day for analyses.

Vehicle:

no

Details on test solutions:

A quantity of 0.3g of SDBR was accurately weighed out and mixed with three litres of DSWL-E in a glass Erlenmeyer flask with a tap atthe bottom, followed by 20h mechanical stirring and 4h standing. Then the medium (Water Soluble Fraction, WSF) was tapped off (the first 200 mL was discarded). New exposure media were prepared at each replacement.
DSWL-E was used as a control medium, together with a stirred DSWL-E control (prepared as the test substance loading rate).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source: commercial hatchery (M.B. Ruysbroek B.V., Noordvliet 159, Maassluis, The Netherlands)
- Length at study initiation: 1.7±0.1 cm
- Weight at study initiation: 0.0542±0.005 g

Test type:

semi-static

Water media type:

freshwater

Remarks:

The medium was prepared with ground water.

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

The animals were counted and checked for mortality after 3, 24, 48, 72 and 96 hours. At the same time the condition (swimming behaviour, colour or any other visually observable morphological or behavioral criterion) of the survivors was compared to that o

Hardness:

213 mg CaCO3/L

Test temperature:

24.8-25.4ºC

pH:

8.2-8.3

Dissolved oxygen:

Lowest measured oxygen concentration: 8.1 mg O2/L

Nominal and measured concentrations:

Overview of analytical results is given below in tabular format.

Details on test conditions:

TEST SYSTEM
The test was performed in 2 liter all-glass beakers, each containing 1.5 liter of exposure medium; the beakers were covered with a watch glass. The exposure media were slightly aerated and replaced daily. At the beginning and at the end of the test, as well as at each replacement time (in the spent media just before replacement and in the newly prepared media just after dosing), the pH and the oxygen concentration of the exposure media were measured.
- No. of organisms per vessel: 10

TEST CONCENTRATIONS
- Test concentration: 100 mg/L (loading rate)
As no adverse effects were expected within the aqueous solubility, only one loading rate of 100 mg/L was tested.

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.02 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Analytics performed t=0h and t=24h

Details on results:

No mortality was observed throughout the study.

Sublethal observations / clinical signs:

No adverse effects were observed throughout the study. 

Validity criteria fulfilled:

yes

Conclusions:

The 96h No Observed Effect Loading rate (NOEL) for acute toxicity of SDBR to fish was found to be greater or equal to a loading rate of 100 mg/L (corresponding to 0.022 mg/L analysed) when tested as a WSF.

Description of key information

The 96h No Observed Effect Loading rate (NOEL) for acute toxicity of SDBR to fish was found to be greater or equal to a loading rate of 100 mg/L (corresponding to 0.022 mg/L analysed) when tested as a WSF.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> mg/L