Registration Dossier
Registration Dossier
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EC number: 251-379-6 | CAS number: 33100-27-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxic potential of crown ethers in Salmonella typhimurium
- Author:
- P.Arenaz
- Year:
- 1 989
- Bibliographic source:
- Mutagenesis vol.4 no.6 pp.437-438, 1989
Materials and methods
- Principles of method if other than guideline:
- The crown ether, S.typhimurium and S9 mix or buffer were mixed with top agar and poured onto minimal glucose plates as described by Maron and Ames (1983). In experiments where S9 was not used, the buffer was omitted from the top agar mixture. Duplicate plates were used for each concentration and each experiment was performed at least twice. The plates were incubated at 37°C for 48 h. The his-independent (his+) colonies arising on the plates were counted by hand using a colony counter (American Optical). The data was then evaluated.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4,10,13-pentaoxacyclopentadecane
- EC Number:
- 251-379-6
- EC Name:
- 1,4,10,13-pentaoxacyclopentadecane
- Cas Number:
- 33100-27-5
- Molecular formula:
- C10H20O5
- IUPAC Name:
- 1,4,7,10,13-pentaoxacyclopentadecane
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 1537
- Species / strain / cell type:
- other: S. typhimurium 1530
- Metabolic activation:
- with and without
Controls
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- other: 2,4,7-trinitrofluorenone 2-aminofluorene
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA100, TA1530, TA98 and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Negative for bacterial reverse mutation assay in S. typhimurium TA100, TA1530, TA98 and TA1537
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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