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EC number: 220-864-4 | CAS number: 2921-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-2 (Acute Dermal Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Chlorpyrifos
- EC Number:
- 220-864-4
- EC Name:
- Chlorpyrifos
- Cas Number:
- 2921-88-2
- Molecular formula:
- C9H11Cl3NO3PS
- IUPAC Name:
- O,O-diethyl O-3,5,6-trichloropyridin-2-yl phosphorothioate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Substance ID: TSN100759
Lot #: 7299412
Purity: 97.6%
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hazleton Research Products, Inc., Kalamazoo, Michigan
- Age at study initiation: 4 to 5 months
- Weight at study initiation: 2.184 to 2.649 kg
- Diet: 4 ounces per day
- Water: ad libitum
- Acclimation period: Atleast 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature: 19±3°C
- Humidity: 39 - 61%
- Air changes: 15 per hr
- Photoperiod: 12 hrs dark/12 hrs light
Administration / exposure
- Type of coverage:
- other: gauze patch was held in place by an elastic rabbit jacket
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: Approximately 10 by 14 cm
- % coverage: 10% of the surface area of the rabbits
- Type of wrap if used: Gauze patch was held in place by an elastic rabbit jacket
REMOVAL OF TEST SUBSTANCE
- Washing: Skin was wiped thoroughly with a water moistened soft disposable towel and dried with a soft disposable towel
- Time after start of exposure: Approximately after 24-hour exposure period
TEST MATERIAL
- For solids, paste formed: Yes, melted to liquid - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg, 5000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations and weighing: The rabbits were weighed prestudy, the day of treatment and on test days 2, 8 and 15
- Necropsy of survivors performed: yes - Statistics:
- Means and standard deviations were calculated for body weights. The data were evaluated for statistical outliers by a sequential test (Grubbs, 1969), however, outliers were not routinely excluded from statistical analysis.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- None
- Clinical signs:
- other: Two males and one female administered 5000 mg/kg had fecal soiling in the perineal area on test days 1 or 2. Treatment-related dermal alterations at the application site consisted of erythema, edema, fissures and scaling in animals administered 5000 mg/kg
- Gross pathology:
- There were no treatment-related gross pathologic observations.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dermal LD50 (Rabbit): >5000 mg/kg
- Executive summary:
The test substance was evaluated for dermal toxicity according to the guidelines OECD 402 and EPA OPPTS 81-2. Five New Zealand White rabbits per sex per dose level received a single, 24-hour, dermal exposure of 2000 or 5000 mg/kg. Parameters evaluated included body weights, in-life observations and gross pathologic evaluation.
All rabbits administered 2000 or 5000 mg/kg (the limit test established by the guidelines) survived. No substantial weight change was observed during the two-week observation period.
Two males and one female administered 5000 mg/kg had fecal soiling in the perineal area on test days 1 or 2. Treatment-related dermal alterations at the application site consisted of erythema, edema, fissures and scaling in animals administered 5000 mg/kg; erythema and scaling were observed in animals administered 2000 mg/kg. All dermal alterations had resolved in all animals by the end of the 14-day observation period, with the exception of scaling that persisted at the application site of one female administered 5000 mg/kg. There were no treatment-related gross pathologic observations.
Under the conditions of this study, the acute dermal LD50 of the test substance was greater than 5000 mg/kg, the limit dose tested, for male and female New Zealand White rabbits.
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