4-O-α-D-glucopyranosyl-D-glucitol

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 11 April 2018 to 15 May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient - Shake Flask Method)

Version / remarks:

version 2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Version / remarks:

version 1995

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

Identification : MALTITOL
CAS No. : 585-88-6
EC No. : 209-567-0
Batch ELEL7
Appearance White powder

Analytical method:

other: Ionic chromatography using amperometric detection

Key result

Type:

log Pow

Partition coefficient:

-3.87

Temp.:

24 °C

pH:

6.6 - 6.8

Details on results:

Three assays, each in duplicate, were performed using different ratio of solvent volumes (2:1; 1:1 and 1:2).
The mean log Pow = -3.87 ; SD = 0.03 ; RSD = 0.76%.
The quality criteria are fulfilled.

 Calibration curve for the aqueous phases

A = 3.54 x C + 2.36 and R=0.9987

 

Calibration curve for the n-octanol phases

A = 6.13 x C + 2.97 and R=0.9997

 

Partition coefficient results

Identification	Peak Area Atest	Mean peak Area Atest	Conc. after Dilution (mg/L) Cd	Dolution Factor Df	Conc. before Dilution (mg/L) C	Coctanol/ Cwater	Log Pow
18-078 A8 A PhaqdilA	6.5663	6.5670	1.19	2500	2974	0.000147	-3.833
	6.5676
18-078 A8 A PhocdilA	8.4371	8.3257	0.87	0.5	0.44
	8.2143
18-078 A8 B Phaqdil	6.5071	6.5023	1.17	2500	2929	0.000136	-3.865
	6.4975
18-078 A8 B PhocdilA	7.8614	7.8678	0.80	0.5	0.40
	7.8741
18-078 A8 C Phaqdil	6.5367	6.5351	1.18	2500	2952	0.000138	-3.860
	6.5334
18-078 A8 C PhocdilA	7.9807	7.9620	0.81	0.5	0.41
	7.9432
18-078 A8 D Phaqdil	6.5591	6.5542	1.19	2500	2965	0.000135	-3.871
	6.5493
18-078 A8 D PhocdilA	7.8795	7.8635	0.80	0.5	0.40
	7.8474
18-078 A8 E Phaqdil	6.5740	6.5732	1.19	2500	2979	0.000119	-3.923
	6.5723
18-078 A8 E PhocdilA	7.2880	7.3352	0.71	0.5	0.36
	7.3823
18-078 A8 F Phaqdil	6.5734	6.5765	1.19	2500	2981	0.000131	-3.882
	6.5795
18-078 A8 F PhocdilA	7.7640	7.7653	0.78	0.5	0.39
	7.7666

 

MeanLogPow	-3.87
SD	0.03
RSD	0.76%

 

Conclusions:

The log Pow of Maltitol was determined as -3.87 ± 0.03 using the shake flask method.

Executive summary:

The coefficient of partition of Maltitol was determined according to the EU method A.8 and OECD 107, and in compliance with GLP.

Since the maltitol is highly hydrophilic, the shake flask method was used to measure its partition between the aqueous phase and the n-octanol phase.

Each phase was first saturated with the other solvent. The concentration of the starting water test item solution was equal to 3.00 g/L. Three assays, each in duplicate, were performed using different ratio of solvent volumes (2:1; 1:1 and 1:2).  Each mixture was shaken using a magnetically stirrer for about 15 minutes at 24 °C ± 1 °C. The temperature during the partition phase was equal to 24 °C ± 1 °C.

Then two phases were separated and the maltitol concentration was analysed in each solvent using the ionic chromatography with amperometric detection. The analysis method was demonstrated to satisfy the SANCO/3030/99 rev.4 regarding the specificity, linearity, accuracy and precision (Report 18 -918023 -004).

The pH of the aqueous phase solutions after the partition was between 6.68 and 6.80 at 22.0 °C.

The mean log Kow is equal to -3.87 ± 0.03 and the relative standard deviation = 0.76%.

The log Kow study is classified as acceptable, and satisfies the guideline requirements of the EU method A.8 and OECD 107.

Description of key information

The log Pow of Maltitol was determined as -3.87 ± 0.03 using the shake flask method.

Key value for chemical safety assessment

Log Kow (Log Pow):

-3.87

at the temperature of:

24 °C

Additional information

The coefficient of partition of Maltitol was determined according to the EU method A.8 and OECD 107, and in compliance with GLP.

Since the maltitol is highly hydrophilic, the shake flask method was used to measure its partition between the aqueous phase and the n-octanol phase.

Each phase was first saturated with the other solvent. The concentration of the starting water test item solution was equal to 3.00 g/L. Three assays, each in duplicate, were performed using different ratio of solvent volumes (2:1; 1:1 and 1:2).  Each mixture was shaken using a magnetically stirrer for about 15 minutes at 24 °C ± 1 °C. Then the two phases were separated and the maltitol concentration was analysed in each solvent using the ionic chromatography with amperometric detection. The analysis method was demonstrated to satisfy the SANCO/3030/99 rev.4 regarding the specificity, linearity, accuracy and precision (Report 18 -918023 -004).

The pH of the aqueous phase solutions after the partition was between 6.68 and 6.80 at 22.0 °C.

The mean log Kow is equal to -3.87 ± 0.03 and the relative standard deviation = 0.76%.