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EC number: 951-219-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation – Irritating in rabbit skin in non-guideline study.
Eye irritation – Not irritating in rabbit (OECD TG 405).
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report which meets basic scientific principles. GLP.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- 24 hour exposure, occlusive wrap
- GLP compliance:
- yes (incl. QA statement)
- Species:
- rabbit
- Strain:
- New Zealand White
- Type of coverage:
- occlusive
- Preparation of test site:
- other: non-abraded and abraded
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0.5 ml
- Duration of treatment / exposure:
- 24 hours
- Observation period:
- Observations were made and 24 and 72 hours and at 7 and 14 days after treatment.
- Number of animals:
- 6 animals
- Details on study design:
- Observations were not made at 48 hours. Therefore, the mean erythema and mean edema scores do not reflect a 24, 48, and 72 hour average, rather they reflect a 24 and 72 hour average.
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- other: 72 hrs
- Score:
- ca. 0.92
- Max. score:
- 2
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: Out of compliance with OECD guidelines: 24 hour exposure, occlusive wrap
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- other: Not Specified
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- other: 24 hrs
- Score:
- ca. 0.5
- Max. score:
- 1
- Reversibility:
- fully reversible within: 14 days
- Remarks on result:
- other: Out of compliance with OECD guidelines: 24 hour exposure, occlusive wrap
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- other: 72 hrs
- Score:
- ca. 0.5
- Max. score:
- 1
- Reversibility:
- fully reversible within: 14 days
- Remarks on result:
- other: Out of compliance with OECD guidelines: 24 hour exposure, occlusive wrap
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- other: 7 days
- Score:
- ca. 0.92
- Max. score:
- 1
- Reversibility:
- fully reversible within: 14 days
- Remarks on result:
- other: Out of compliance with OECD guidelines: 24 hour exposure, occlusive wrap
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- 24/72 hr time point average
- Time point:
- other: 24/72 hr
- Score:
- ca. 0.5
- Max. score:
- 0.5
- Reversibility:
- fully reversible within: 14 days
- Remarks on result:
- other: Out of compliance with OECD guidelines: 24 hour exposure, occlusive wrap
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- other: Not Specified
- Other effects:
- Unleaded motor gasoline was administered via an occlusive dermal patch on abraded skin. The erythema scores at 24 and 72 hours and at 7 and 14 days were 0.0, 1.0, 0.17, and 0.0, respectively. Erythema was fully reversible within 14 days after treatment. The edema scores at 24 and 72 hours and at 7 and 14 days were 0.5, 0.5, 1.08, and 0.0, respectively. Edema was fully reversible within 14 days after treatment.
- Interpretation of results:
- moderately irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- The results of this study cannot be directly interpreted for classification of skin irritation due to deviations from the EEC regulatory protocol, consisting of the use of a 24 hr, rather than a 4 hr, exposure period and the use of an occlusive, rather than a semi-occlusive, wrap.
- Executive summary:
Unleaded motor gasoline (API PS-6) was administered via occlusive dermal patch on non-abraded and abraded skin of 6 New Zealand White rabbits at doses of 0.5 ml for 24 hours to assess dermal irritation. Clinical observations were made at 24 and 72 hours and at 7 and 14 days after treatment. Unleaded motor gasoline produced dermal irritation (erythema and edema) to the skin of rabbits as a result of 24 -hour exposure. Erythema was fully reversible within 7 days and edema was fully reversible within 14 days.
The erythema and edema scores of unleaded motor gasoline (API PS-6) indicated a potential to cause dermal irritation.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Acceptable,well-documented study report similar or equivalent to OECD 405. GLP.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- Amount applied: 0.1 ml
- Duration of treatment / exposure:
- The eyes of 6 animals were left unrinsed after administration. The eye of the 3 animals was rinsed for one minute 20 to 30 seconds after test material instillation.
- Observation period (in vivo):
- Observations were made at 24, 48 and 72 hours after treatment.
- Number of animals or in vitro replicates:
- unrinsed: 6 animals
rinsed: 3 animals - Irritation parameter:
- overall irritation score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: unrinsed; max possible score of 110
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: unrinsed; max possible score of 3
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: unrinsed; max possible score of 4
- Irritation parameter:
- cornea opacity score
- Remarks:
- opacity
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: unrinsed; max possible score of 4
- Irritation parameter:
- iris score
- Remarks:
- lesion
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: unrinsed; max possible score of 2
- Other effects:
- Unleaded motor gasoline (API PS-6) was also instilled into the eyes of 3 rabbits, which were rinsed 20-30 seconds after dosing. This method is not in compliance with OECD TG 405 and is not appropriate for classification purposes. The overall irritation score was approximately 0.0 (max score out of 110 possible). The mean (24, 48, and 72 hr average) conjunctivae redness, chemosis, corneal opacity, and iris lesion scores were 0.0, 0.0, 0, and 0, respectively.
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The mean corneal opacity, iris lesion, conjunctivae redness, and chemosis scores were 0, 0, 0.0, and 0.0, respectively. These findings do not warrant classification of unleaded motor gasoline (API PS-6) as an ocular irritant under Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP).
- Executive summary:
Unleaded motor gasoline (API PS-6) was administered to one eye of six male New Zealand White rabbits to assess for ocular irritation. Observations were made at 24, 48 and 72 hours after treatment. Ocular damage was assessed and scored according to the Draize eye test.Ocular irritation was not observed after treatment and the overall irritation score was 0.0. The mean corneal opacity, iris lesion, conjunctivae redness, and chemosis scores were 0, 0, 0.0, and 0.0, respectively. These findings do not warrant classification of unleaded motor gasoline (API PS-6) as an ocular irritant under Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP).
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
The human literature indicates that gasoline can cause dermal irritation but does not appear to be severe dermal irritants or corrosive agents. There is some evidence of eye irritation associated with vapor exposure at levels equal to and greater than 200 ppm, but the effects were mild, and the dose-response information was not conclusive.
Similarly, gasoline produced some dermal irritation but not corrosion when applied to rabbit skin. The level of irritation was enhanced when the test materials were applied under occluded patch.. There was very little evidence of irritation when gasoline was instilled into rabbit eyes.
Effects on skin irritation/corrosion: irritating
Justification for classification or non-classification
The animal studies were conducted under regulatory guidelines and in accordance with good laboratory practice recommendations. The data can be used without restriction for regulatory purposes and support classification of gasoline and naphtha blending stocks as Skin Irritant 2 according to EU CLP Regulation (EC No. 1272/2008).
Gasoline and naphtha blending stocks do not meet the criteria for classification as an eye irritant according to the EU CLP Regulation (EC No. 1272/2008).
There is no need to conduct additional animal testing for these endpoints.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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