Poly(oxy-1,2-ethanediyl), α,α'-(iminodi-2,1-ethanediyl)bis[.omega.-hydroxy-, N-[3-(C10-16-alkyloxy)propyl] derivs., di-Et sulfate-quaternized

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name: Poly(oxy-1,2-ethanediyl), .alpha.,.alpha.'-(iminodi-2,1-ethanediyl)bis[.omega.-hydroxy-, N-[3-(C10-16-alkyloxy)propyl] derivs., di-Et sulfate-quaternized
Product Description: C10-16-alkyletherpropylamine, ethoxylated, DES Quat
CAS No.: 70983-58-3
Physical state: yellowish to amber viscous liquid at 20 °C
Batch No.: PFS-755-173
Re-certification date of batch: 19 April 2018
Purity: 100 % (UVCB)
Color, Gardner 8.8
pH, 5% in water 5.76
Acid Value , mg KOH/g 20.1
Moisture, % 0.127
Total Amine, mg/g 11.18
Viscosity,cps, #4@60,25C 3280
Appearance @25C pass
Stability: stable under test conditions
Storage condition of test material: Room temperature, protected from light

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

Activated sludge from the municipal wastewater treatment plant Breisgauer Bucht was used as inoculum with a concentration corresponding to 30 mg dry solids per litre. The treatment plant clarifies predominantly domestic wastewater and has a capacity of 600.000 inhabitant equivalents. Sampling date of activated sludge was on 4 July 2017. The dry solid content of the activated sludge was 3.9 g/L. It was determined by weight measurements after drying at 105°C for 5 hours (mean of triplicate measurements). The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge.

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

-0.3

Sampling time:

28 d

Details on results:

No degradation of the test item could be observed within the test duration. The degradation at the end of the test was -0.3% (28 d after acidification, mean of two replicates). The test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window). The degradation of the toxicity control after 14 days was 39.3%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Results with reference substance:

The reference compound sodium benzoate reached the pass levels for ready biodegradability within 4 days.

Table 1: Ultimate biodegradation after x days [% of ThCO2]

reactor	day	0	4	7	11	14	21	28	29
11	Test flasks	0	-1.8	5.8	4.4	4.2	4.3	2.9	2.3
12		0	-3.6	0.6	1.1	0.5	-1.4	-2.6	-2.9
13*		0	-0.4	5.3	5.7	7.1	10.6	17.5	18.0
4	Reference flasks	0	75.4	86.3	88.4	89.5	90.6	93.1	96.8
5		0	77.7	82.7	84.7	87.1	86.0	90.4	94.6
6		0	72.8	80 .7	86.7	87.8	87.6	88.9	91.8
14	Toxicity control - test item & reference item	0	32.1	37.7	40.7	39.3	40.4	41.4	41.4

* outlier

Table 2: Mean CO2 evolution of blanks after x days

Day	0	4	7	11	14	21	28	29
CO2 -evolution [mg/l]	0	9.6	14.2	18.5	21.7	25.6	30.5	31.6

Validity criteria fulfilled:

yes

Remarks:

The temperature during the test was 19.2 – 22.3°C and therefore below the range required by the guideline (22 ± 2°C). The underrun of the required temperature occurred between day 15 and day 20 of the study and was therefore only temporarily.

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

No degradation of the test item could be observed within the test duration. The degradation at the end of the test was -0.3% (28 d after acidification, mean of two replicates). The test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window). The degradation of the toxicity control after 14 days was 39.3%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Executive summary:

In a Klimisch 1 OECD 301 B GLP study no degradation of the test item could be observed within the test duration. The degradation at the end of the test was -0.3% (28 d after acidification, mean of two replicates). The test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window). The degradation of the toxicity control after 14 days was 39.3%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Description of key information

No degradation of the test item could be observed within the test duration. The degradation at the end of the test was -0.3% (28 d after acidification, mean of two replicates). The test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window). The degradation of the toxicity control after 14 days was 39.3%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed