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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar 25 - Jun 20, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure and at the renewal (0 and 24 h), samples of the freshly prepared test item concentrations were taken and analyzed.
At renewal and at the end of the exposure (24 and 48 h), sampled of the 24 h old media were taken directly fron the test vessels and analyzed. - Vehicle:
- yes
- Remarks:
- dilution water
- Details on test solutions:
- The study was conducted under semi-static conditions over a period of 48 hours with a saturated solution of the test item and a further four dilution levels (nominal: 6.25 – 12.5 – 25.0 – 50.0 - 100 %) of the saturated solution prepared in a geometric series with a separation factor of 2. The saturated solution was prepared following the recommendations of the OECD guidance document on aquatic toxicity testing of difficult substances and mixtures (OECD series on testing and assessment no. 23, ENV/JM/MONO(2000)6/REV1).
The saturated solution had a positive Tyndall effect and was slightly turbid throughout the exposure. The further concentration levels (6.25 to 50 % of the saturated solution) were visually clear throughout the exposure.
Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control.
The concentrations of the test item were analytically verified via HPLC-DAD in the fresh media of all concentration levels and in the control at the start of the exposure and at the renewal (0 and 24 hours) as well as in the 24-hours old media at the renewal and at the end of the test (24 and 48 hours). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden und Lufthygiene (WaBoLu), Berlin, Germany
- Age: < 24 h
FEEDING DURING TEST
- Food type: 5 x per week ad libitum with a mix of unicellular green algae - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 0 h: 254 mg CaCO3/L
24 h: 266 mg CaCO3/L - Test temperature:
- 19.8 - 20.1 °C
- pH:
- 0 h: 7.62
24 h: 7.24 - Dissolved oxygen:
- 0 h: 8.66 mg/L
24 h: 8.53 mg/L - Conductivity:
- 0 h: 668 µS/cm
24 h: 653 µS/cm - Nominal and measured concentrations:
- nominal: 6.25, 12.5, 25.0, 50.0, 100.0 mg/L
measured: 0.156, 0.206, 0.512, 0.905, 1.92 mg/L - Details on test conditions:
- Test vessels: Glass beakers, 50 mL capacity, loosely covered with watch glasses
Test volume: 20 mL
Number of daphnids and replicates: 20 daphnids, divided into 4 replicates, each with 5 daphnids were used for each concentration level and control.
Application: 20 g test solution per replicate was weighed out into each test vessel. This corresponds to 20 mL per test vessel. The daphnids were inserted with a samll amount of dilution water (start of the exposure) or test solution (water renewal) by pipette.
Renewal of the test solutions: The test solutions were renewed after 24 h. For this purpose, a second set of test vessels was filled with the freshly prepared test solutions and the daphnids were transferred by pipette. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate; in a periodically (once per months) conducted test to prove validity of test system and conditions
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 513 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- EC50-Value (with 95 % confidence limits) of the reference item potassium dichromate: EC50 = 2.03 mg/L (1.00 -4.00)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48h) based on the geometric mean measured concentrations of the test item was at 0.513 mg/L (95 % confidence limits: 0.481 – 0.544 mg/L).
- Executive summary:
The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 201.
The study was conducted under semi-static conditions over a period of 48 hours with a saturated solution of the test item with a nominal loading of 100 mg test item/L and further four dilution levels (nominal: 6.25 – 12.5 – 25.0 – 50.0 - 100 %) of the saturated solution prepared in a geometric series with a separation factor of 2.
Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each dilution level and the control. The concentrations of the test item were analytically verified via HPLC-DAD in the fresh media at the start of the exposure and at the renewal of the test solutions (0 and 24 hours) and in the old media at the renewal and at the end of the test (24 and 48 hours) in all dilution levels and in the control. The geometric mean measured concentrations are 0.156 - 0.206 - 0.512 - 0.905 - 1.92 mg/L.
The EC50 (48h) based on the geometric mean measured concentrations of the test item was at 0.513 mg/L (95 % confidence limits: 0.481 – 0.544 mg/L).
Reference
1) Immobilization Rates after
24 and 48 hours of Exposure in the Definitive Test
Geometric mean measured test item concentration |
IMMOBILIZATION [%] |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
1.92 |
40 |
100 |
80 |
20 |
60 |
100 |
100 |
100 |
100 |
100 |
0.905 |
0 |
100 |
40 |
80 |
55 |
80 |
100 |
100 |
100 |
95 |
0.512 |
0 |
0 |
0 |
0 |
0 |
40 |
60 |
60 |
40 |
50 |
0.260 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.156 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2) Measured Concentrations
during the Definitive Test
Sampling |
Fresh media, |
Old media, |
Fresh media, |
Old media, |
|
||
Dilution level [%] |
Test material |
||||||
Meas. conc. [mg/L] |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
Meas. conc. [mg/L] |
% |
Geometric mean measured concentration [mg/L] |
|
100 |
1.52 |
0.542 |
36 |
8.25 |
2.00 |
24 |
1.92 |
50.0 |
1.07 |
0.257 |
24 |
5.60 |
0.436 |
8 |
0.905 |
25.0 |
0.474 |
0.175 |
37 |
2.77 |
0.299 |
11 |
0.512 |
12.5 |
0.182 |
0.101 |
56 |
1.35 |
0.183 |
14 |
0.260 |
6.25 |
0.0987 |
0.0554 |
56 |
0.731 |
0.147 |
20 |
0.156 |
Control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
|
Meas. conc. = measured concentration of the test item, enrichment and dilution factors taken into account
% = percentage of the initially measured concentration of the test item
LOQ = limit of quantification of the analytical method (1 µg test item/L)
Description of key information
The EC50 (48h) based on the geometric mean measured concentrations of the test item was at 0.513 mg/L (95 % confidence limits: 0.481 – 0.544 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 513 µg/L
Additional information
The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 201.
The study was conducted under semi-static conditions over a period of 48 hours with a saturated solution of the test item with a nominal loading of 100 mg test item/L and further four dilution levels (nominal: 6.25 – 12.5 – 25.0 – 50.0 - 100 %) of the saturated solution prepared in a geometric series with a separation factor of 2.
Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each dilution level and the control. The concentrations of the test item were analytically verified via HPLC-DAD in the fresh media at the start of the exposure and at the renewal of the test solutions (0 and 24 hours) and in the old media at the renewal and at the end of the test (24 and 48 hours) in all dilution levels and in the control. The geometric mean measured concentrations are 0.156 - 0.206 - 0.512 - 0.905 - 1.92 mg/L.
The EC50 (48h) based on the geometric mean measured concentrations of the test item was at 0.513 mg/L (95 % confidence limits: 0.481 – 0.544 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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