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EC number: 617-116-8 | CAS number: 80573-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 13th 2018 - June 20th 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch: 0400
Manufacturing date: 04/2018
Expiry date: 04/2019 - Analytical monitoring:
- no
- Details on sampling:
- After the exposure period each sample was transferred from the aeration vessel to a bottle fitted with a magnetic stirrer, An oxygen probe was inserted into the neck of the bottle to measure the concentration of dissolved oxygen.
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution of the test item was prepared after 1 hour of sonication and 24 hours of stirring at 35°C at a concentration of 2000 mg/l. Bottles were prepared with deionized water, synthetic sewage feed at 3 different concentrations of test item ( 1000 mg/L, 100 mg/L and 10 mg/L) using data of solubility in water. pH of the solutions was checked. The mud was added before starting the test.
PREAPRATION OF THE REFERENCE SUBSTANCE:
A stock solution of reference substance 3,5 Dichlorophenol was prepared at a concentration of 1000 mg/l in deionized water. 3 different concentrations ( 50 mg/L, 10 mg/L and 1 mg/L) were prepared in the same way of the test item.
PREPARATION OF CONTROLS:
Blank controls: A blank control was used consisting of deionised water and an equal volume of activated slide and synthetic medium without reference or test item.
Abiotic controls: Abiotic control was prepared with the highest concentration amount of test item with the same amount of synthetic sewage feed and without the inoculum. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
A sample of aerobic mud was selected by the mixed treatment plant of urban about (66%) and industrial (about 34%) liquid sewage situated in San Rocco near Monza (MB). The plant of treatmnet is managed by Brianzacque Srl (Monza-Italia)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20°C ± 2°C
- pH:
- 7.47 - 7.68
- Nominal and measured concentrations:
- Nominal test concentrations of 1000 mg/L, 100 mg/L and 10 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass bottle
- Aeration: Vessels were aerated to keep the dissolved oxygen concentration above 60-70% saturation.
- No. of vessels per concentration (replicates): 3
- No. of vessels per blank control (replicates): 3
- No. of vessels per test reference (replicates): 3
- No. of vessels per abiotic control (replicates): 3
- Sludge concentration (weight of dry solids per volume): The suspended solids in the mud solution was 3 g/l to have in the final mixture a concentration of 1.5 g/l
- Weight of dry solids per volume of reaction mixture per unit of time:
-Synethtic sewage feed:
Peptone: 16 g/L
Meat extract: 11 g/L
Urea: 3 g/L
Sodium chloride: 0.7 g/L
Calcium chloride dihydrate: 0.4 g/L
Magneisum sulphate heptahydrate: 0.2 g/L
Anhydrous potassium monohydrogen phosphate: 2.8 g/L
Deionised water: To 1000 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water
OTHER TEST CONDITIONS
- Adjustment of pH: pH was corrected with NaOH in 100 and 1000 mg/L vessels. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes. Validity criteria was met as the EC50 o f 3,5 Dichlorophenol was between 2 mg/l and 25 mg/l
- Relevant effect levels: EC50 = 6.582 mg/L for total respiration - Validity criteria fulfilled:
- yes
- Remarks:
- Blank controls oxygen uptake rate: 25.40 mg O2/g activated sludge in 1h; coefficient of variation of O2 uptake rate in controls: 5.33%.; EC50 of 3,5-Dichlorophenol: 6.582 mg/L; O2 consumption rate for abiotic control: 0.20 mg/L h
- Conclusions:
- The EC50 of Balsalazide acid after 3 hours was > 1000 mg/L.
Reference
Table 1: Mean percent inhibition total respiration rate of the test item Balsalazide acid
Concentration |
% of inhibition total respiration rate of Balsalazide acid |
10 mg/L |
11.68 |
100 mg/L |
21.26 |
1000 mg/L |
21.92 |
Table 2: Mean percent inhibition total respiration rate of the reference substance
Concentration |
% of inhibition total respiration rate of Balsalazide acid |
1mg/L |
21.00 |
10 mg/L |
54.07 |
50 mg/L |
83.86 |
Description of key information
The effect of Balsalazide acid on micro-organisms from activated sludge was determined in accordance with OECD Guideline 209. The 3 hour EC50 of Balsalazide acid was found to be >1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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