Methyl 3-aminocrotonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 January 2000 - 9 March 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984 Part 1

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Test item code: LZ937.00
Expiration date: 21 May 2000
Delivery date: 11 October 1999
Test material was stored at ambient temperature in the dark.
Physical state: White crystalline solid
Storage conditions: Stored at ambient temperature in the dark

Analytical monitoring:

yes

Vehicle:

no

Remarks:

water

Details on test solutions:

Test solutions were prepared from a 1000 p.p.m. stock solution. The 1000 p.p.m. stock solution was prepared by adding a weighed amount of LZ937 (249.5 mg) to a 250 ml volumetric flask and bringing to volume with Daphnia medium. The stock solution was ultrasonicated for ca 5-10 min to ensure dispersion/solubility. Serial dilutions of this stock were then made with Daphnia medium to produce the required test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (water flea) used in this study are continuously cultured inhouse by acyclical parthenogenesis. At initiation of exposure neonates were between 6 and 24 h old. Daphnia cultures were maintained in a synthetic
medium as used in the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24 h

Hardness:

146 mg CaCO3

Test temperature:

20°C - 21.3°C

pH:

between 8.32 and 8.69

Dissolved oxygen:

between 87% and 93.2%

Salinity:

not applicable

Conductivity:

between 478 µs and 608 µs

Nominal and measured concentrations:

Nominal 100 p.p.m.

Details on test conditions:

Test vessels were maintained in a temperature-controlled laboratory between 22 and 23°C during the definitive test. A light cycle of 16 h light and 8 h dark was in operation throughout the exposure and culture periods. Fluorescent tubes provided artificial daylight illumination. The temperature was recorded at a maximum of 23°C during the test, exceeding 22°C stated in the protocol. This deviation did not affect the validity of the test.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 24h exposure, 10 (100%) immobile Daphnia neonates at 1000 ppm and 1 (10%) immobile neonate at 100 p.p.m were observed. No further immobile Daphnia neonates were observed for any concentration in this test after 48 h. The 48 h static limit test was conducted at nominal whole product concentration of 100 p.p.m. with an untreated control (0 ppm). No significant numbers of immobile Daphnia were recorded during the study. A single neonate (5%) was observed in the limit test after 24 h exposure. This low level of effect is not regarded to be indicative of a test material effect.

Results with reference substance (positive control):

not applicable

All test solutions were clear and colourless. No precipitation of test material was observed. This indicates full dispersion or dissolution of the test material in the test medium. All results are based on nominal concentrations in the absence of analytical results.

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 is >100 mg/L based on nominal concentrations.

Executive summary:

The study was conducted to determine the acute toxicity (EC50) of LZ937 to the aquatic invertebrate Daphnia magna. The study was conducted under static conditions for 48 h, in accordance with OECD Guideline 202 and EC Guideline C2. A preliminary range finding study indicated that LZ937 was of low toxicity to neonates of Daphnia magna, at a concentration up to 1000 p.p.m. the maximum test concentration tested. A limit study was then conducted at 100 p.p.m. nominal, of LZ937. No significant effects (immobilisation) were observed in the limit study, in either the test or control groups. A single neonate (5%) was observed to be immobile in one test vessel at 24 hours. This is not regarded as an effect of the test material. Throughout the study all test solutions were clear and colourless indicating full dissolution or dispersion of LZ937 in the test medium. The results of this study are based on nominal concentrations.

The results were: 24 and 48h EC50 >100 mg/L nominal of LZ937 and NOEC 100 mg/L nominal of LZ937: Determination of an analytical method was possible.

Description of key information

The study was conducted under static conditions for 48 h, in accordance with OECD Guideline 202 and EC Guideline C2. A preliminary range finding study indicated that LZ937 was of low toxicity to neonates of Daphnia magna, at a concentration up to 1000 p.p.m. the maximum test concentration tested. A limit study was then conducted at 100 ppm nominal, of LZ937. No significant effects (immobilisation) were observed in the limit study, in either the test or control groups. The results of this study are based on nominal concentrations: 24 and 48h EC50 >100 mg/L nominal of LZ937 and NOEC 100 mg/L nominal of LZ937.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L