Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 21, 2018 - April 19, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
2242-62-5
- Expiration date of the lot/batch:
November 3, 2020
- Purity test date:85.3%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
ambient conditions. - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Test water samples were collected from the batches of test solution prepared for each treatment and control group at the beginning of the test, and from two of the four replicate test chambers in each treatment and control group at 48 hours (± 1 hour) to measure concentrations of the test substance. Samples (8.0 mL) were collected from mid-depth, placed in glass vials containing 2.0 mL of 0.5% formic acid in methanol and processed immediately for analysis.
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: The cladoceran, Daphnia magna, was selected as the test species for this study.
- Strain/clone:
- Justification for species other than prescribed by test guideline:
- Source: neonates obtained from cultures maintained by EAG Laboratories in Easton, Maryland.
- Age of parental stock (mean and range, SD): Daphnids used in the test were <24-hour old
- Feeding during test: The adults were fed during the 24-hour period prior to test initiation, but neonates were not fed during the test.
- Food type: mixture of yeast, cereal grass media and trout chow (YCT), supplemented with a vitamin stock solution and a suspension of the freshwater green alga, Raphidocelis subcapitata.
- Amount:
- Frequency:Daphnids in the cultures were fed daily
ACCLIMATION
- Acclimation period:Adult daphnids were cultured in water from the same source and at approximately the same temperature as used during the test. During the 2-week period immediately preceding the test,
- Acclimation conditions (same as test or not):
- Type and amount of food: mixture of yeast, cereal grass media and trout chow (YCT), supplemented with a vitamin stock solution and a suspension of the freshwater green alga, Raphidocelis subcapitata.
- Feeding frequency: Daphnids in the cultures were fed daily
- Health during acclimation (any mortality observed): The four adult daphnids used to supply neonates for the test were held for 14 days prior to collection of the juveniles for testing, and had each produced at least two previous broods. Adult daphnids in the culture had produced an average of at least three young per adult per day over the 7-day period prior to the test. The adults showed no signs of disease or stress, no ephippia were produced during the holding period, and mortality in the culture stock was 0% in the two-day period prior to test initiation
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Observations of immobility and other signs of toxicity were made approximately 3, 24 and 48 hours after test initiation
- Hardness:
- 128 mg/L as CaCO3
- Test temperature:
- Temperature Range: 19.0 – 20.3°C
- pH:
- pH Range: 8.1 – 8.5
- Dissolved oxygen:
- Dissolved Oxygen Concentration: ≥90% ASV; no aeration
- Salinity:
- Alkalinity 178 mg/L as CaCO3
- Conductivity:
- 312 μS/cm
- Nominal and measured concentrations:
- TEST CONCENTRATIONS: Nominal Mean Measured
Negative Control < LOQ
6.3 mg a.i./L 6.4 mg a.i./L
13 mg a.i./L 13 mg a.i./L
25 mg a.i./L 24 mg a.i./L
50 mg a.i./L 50 mg a.i./L
100 mg a.i./L 102 mg a.i./L - Details on test conditions:
- TEST SYSTEM
- Test vessel: chambers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: not specify
- Aeration: no aeration
- No. of organisms per vessel:five daphnids in each test chamber
- No. of vessels per concentration (replicates):Four replicate test chambers were maintained in each treatment group
- No. of vessels per control (replicates):Four replicate test chambers were maintained in control group
- No. of vessels per vehicle control (replicates): no
- Biomass loading rate:Loading in each test chamber during the test was approximately 40 mL of test solution per daphnid.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:freshwater obtained from a well approximately 40 meters deep located on the EAG Laboratories site in Easton, Maryland. The well water was passed through a sand filter to remove particles greater than approximately 25 m, and pumped into a 37,800-L storage tank where the water was aerated with spray nozzles. Prior to use in the test system, the water was filtered to 0.45 m to remove fine particles and was passed through an ultraviolet (UV) sterilizer.
- Total organic carbon:<1mg C/L
- Particulate matter:
- Metals:Analysis table is provided in the background material
- Pesticides:Analysis table is provided in the background material
- Chlorine:Analysis table is provided in the background material
- Alkalinity: 187 mg/L as CaCO3
- Ca/mg ratio:Analysis table is provided in the background material
- Conductivity: 332-350 μS/cm
- Salinity:Analysis table is provided in the background material
- Intervals of water quality measurement: periodically.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod:16 hours of light
and 8 hours of darkness. A 30-minute transition period of low light intensity was provided when lights went on and off to avoid sudden changes in light intensity.
- Light intensity:559 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: The range-finding study was conducted at nominal concentrations of 0.16, 0.80, 4.0, 20 and 100 mg/L for 48 hours under static test conditions.
- Results used to determine the conditions for the definitive study: Percent immobility in the 0.16, 0.80, 4.0, 20 and 100 mg/L treatment groups at test termination was 0, 0, 0, 0 and 0%, respectively. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Percent immobility in the 6.3, 13, 25, 50 and 100 mg a.i./L treatment groups at test termination was 0, 0, 0, 0 and 0%, respectively. Signs of toxicity observed among the surviving daphnids in the 100 mg a.i./L treatment group at test termination included lethargy.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The cladoceran, Daphnia magna, was exposed for 48 hours under static conditions to five nominal concentrations of E17-194T ranging from 6.3 to 100 mg a.i./L. Based on the nominal
concentrations, the 48-hour EC50 value was >100 mg a.i./L, the highest concentration tested. The 48-hour no-immobility concentration and the NOEC were both 100 mg a.i./L. - Executive summary:
The cladoceran, Daphnia magna, was exposed for 48 hours under static conditions to five nominal concentrations of E17-194T ranging from 6.3 to 100 mg a.i./L. Based on the nominal concentrations, the 48-hour EC50 value was >100 mg a.i./L, the highest concentration tested. The 48-hour no-immobility concentration and the NOEC were both 100 mg a.i./L.
Reference
Description of key information
The cladoceran, Daphnia magna, was exposed for 48 hours under static conditions to five nominal concentrations of E17-194T ranging from 6.3 to 100 mg a.i./L. Based on the nominal concentrations, the 48-hour EC50 value was >100 mg a.i./L, the highest concentration tested. The 48-hour no-immobility concentration and the NOEC were both 100 mg a.i./L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.