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EC number: 224-583-8 | CAS number: 4419-11-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18.10.17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- yes
- Remarks:
- deviations were stated as uncritical
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2'-azobis[2,4-dimethylvaleronitrile]
- EC Number:
- 224-583-8
- EC Name:
- 2,2'-azobis[2,4-dimethylvaleronitrile]
- Cas Number:
- 4419-11-8
- Molecular formula:
- C14H24N4
- IUPAC Name:
- 2-[(E)-2-(1-cyano-1,3-dimethylbutyl)diazen-1-yl]-2,4-dimethylpentanenitrile
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Name and location of sewage treatment plant where inoculum was collected: sludge was taken from
the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NWLachen-
Speyerdorf.
- Method of cultivation:
- Preparation of inoculum for exposure:
- Pretreatment: filtrated, washed with tap water 3 times and re-suspended in tap water. The activated
sludge was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed /L.
- Initial biomass concentration: #01 (pre-test): 2.76 g suspended solids/L dw, respectively, 1.38 g suspended solids/L
dw for the test; #02 (main test): 2.92 g suspended solids/L dw, respectively, 1.46 g suspended solids/L
dw for the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 0.71 mmol/L
- Test temperature:
- #01: 20.2 – 21.4 °C (first experiment (pre-test))
#02: 19.0 – 21.4 °C (second experiment (main test)) - pH:
- 8.26
- Conductivity:
- 174 µS/cm (25 °C)
- Nominal and measured concentrations:
- #01: 1000, 100, 10, 1 mg/L
#02: 3200, 1000, 320, 100, 32 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, 500 mL
- Aeration: purified air, using Pasteur pipettes, flow approx. 0.75 L/min
- Type of flow-through (e.g. peristaltic or proportional diluter): -
- Renewal rate of test solution (frequency/flow rate): -
- No. of organisms per vessel: 250 mL inoculum
- No. of vessels per concentration (replicates): #01: positive control & lowest test concentration: one
replicate, five replicates (highest concentrations); #02: 1 rep/treatment (positive control), 5 rep/treatment (test item)
- No. of vessels per control (replicates): two replicates before and two after measuring positive control
and test item, respectively
- No. of vessels per vehicle control (replicates): -
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: 0.6 mg/l
- Particulate matter:
- Metals:< 0.007 mg/L
- Pesticides: < LOD
- Chlorine: 9 mg/L
- Alkalinity: not stated
- Ca/mg ratio: 12
- Conductivity: 174 μS/cm
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: -
- Photoperiod: -
- Light intensity: -
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : inhibition of respirati
on rate
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
Test concentrations:1000, 100, 10, 1 mg/L (nominal)
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- 3,5- DCP (CAS# 591-35-5)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 280 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- 3,5-Dichlorophenol was used as positive control. Four concentrations were tested; an EC50 of 7.6 mg/L (95% confidence interval: 5.5 – 9.5 mg/L) was determined, which lies within the recommended range of 2 – 25 mg/L stated in the OECD guideline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
Two experiments were performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.76 g suspended solids/L, giving a concentration of 1.38 g suspended solids/L in the test.
3,5-Dichlorophenol was used as positive control. Four concentrations were tested; an EC50 of 7.6 mg/L (95 % confidence interval: 5.5 – 9.5 mg/L) was determined, which lies within the recommended range of 2 – 25 mg/L stated in the OECD guideline.
Inhibition at the concentration 1000 mg/L did not rise above 19 %. Nevertheless, at 1000 mg/L clearly toxicity occurred. Therefore one additional experiment was performed under the same test conditions.
In the second experiment (main test) the test item was tested using 5 concentrations ranging from 3200 to 32 mg/L nominal concentration.
The dry matter of the inoculum was determined as 2.92 g suspended solids/L, giving a concentration of 1.46 g suspended solids/L in the test.
For the positive control an EC50 of 8.7 mg/L (95 % confidence interval: 5.0 – 12 mg/L) was determined. The value lies within the recommended range of the OECD guideline.
The following results for the test item2,2'-Azobis(2,4-dimethylvaleronitrile)were determined:
3h NOEC=320 mg/L
3h EC10 = 280 mg/L (Confidence Interval: 220 – 350 mg/L)
3h EC50>3200 mg/L (Confidence Interval: not determinable)
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