[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

EC Number:

229-995-1

EC Name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Cas Number:

6891-44-7

Molecular formula:

C9H18NO2.CH3O4S

IUPAC Name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Constituent 2

Reference substance name:

Ethanaminium, N,N,N-trimethyl-2-[(2-methyl-1-oxo-2-propenyl)oxy]-, methyl sulfate

IUPAC Name:

Ethanaminium, N,N,N-trimethyl-2-[(2-methyl-1-oxo-2-propenyl)oxy]-, methyl sulfate

Details on test material:

- Name of test material (as cited in study report): MADAMDMS
- Physical state: Uncoloured to strow coloured liquid
- Analytical purity: 80% in water
- Composition of test material, percentage of components: 80% solution in water
- Lot/batch No.: no data
- Storage condition of test material: In darkness and at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: CHODAT86-8l SAG
- Source : AQUARIUM DE TROUVILLE (17 rue de Paris - F - 14360 TROUVILLE).
- Age of inoculum (at test initiation): approximately 72-hour old algal sub-culture
- Method of cultivation: axenic subcultures made at regular intervals.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

< 90 mg de Ca C03 per litre of culture medium.

Test temperature:

23°C

pH:

6.87-6.95

Salinity:

none

Nominal and measured concentrations:

0 (control, 1, 10 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Initial cells density: 10,000
- No. of vessels per concentration (replicates): 3
- No. of vessels per vehicle control (replicates): 6


TEST MEDIUM / WATER PARAMETERS
Culture medium without test substance: composition (mg of salt per litre of dilution water)
Ca(N03)2 .4 H20 = 40, KN03 =100, MgS04•7H20 = 30, K2HP04 = 40, CuS04 .5H20 = 0.015, (NH4)6 MO7 O24. 4 H2O = 0.030
ZnS04•7H20 = 0.030, CoCl2• 6 H20 = 0.030, Mn(N03)2 . 4 H20 = 0.030, C6H807• H20 = 0.030, H3BO3 = 0.030
Ammonium iron III citrate (Fe203 content: 29 - 32 % ) = 0.8125, FeS04• 7H20 = 0.3125, FeC13. 6H20 = 0.3125

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: with with HCl 0.1N.
- Photoperiod and Light intensity and quality: artificial continuous white lighting, providing from 6000 lux to 6100 lux;



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth of the culture at 24; 48, and 72 h
- Determination of cell concentrations: no data

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

No abnormality (deposits, films, ... ) was recorded in test media, up to 100.0 mg/L during the study period.
- Growth inhibition : EC A (I) 50 - 72 hours is included between 10.0 and 100.0 mg/L
Growth rate inhibition - ECµ (I) 50 - 72 hours is higher than 100.0 mg/L.

Any other information on results incl. tables

	Algal concentration
concentration (mg/l	Start	24 hours	48 hours	72 hours	% growth inhibition/control
0	10 000	103,472	725,000	2,867,361	0
1	10 000	93,055	708,333	2,586,111	7
10	10 000	84,722	513,889	2,375,000	21
100	10 000	69,445	194,444	1,294,444	60

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Growth inhibition : EC A (I) 50 - 72 hours is included between 10.0 and 100.0 mg/L
Growth rate inhibition - ECµ (I) 50 - 72 hours is higher than 100.0 mg/L.

Executive summary:

MADAM DMS was tested for evaluation of the inhibition of growth and reproduction induced by a test susbtance in an unicellular freshwater green alga over an exposure period of 72 hours

Blue-green algae (Scenedesmus subspicatus) were exposed in a reconstituted medium at 23^oC for 72 hours. The pH was carefully monitored throughout the study. Concentrations of 0.0, 1.0, 10, and 100.0 mg/l of test substance were used. Algal concentrations were measured after 24, 48 and 72 hours.

Result:

Growth inhibition : EC A (I) 50 - 72 hours is included between 10.0 and 100.0 mg/L

Growth rate inhibition - ECµ (I) 50 - 72 hours is higher than 100.0 mg/L.