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EC number: 807-888-6 | CAS number: 110621-40-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 09 2016 - October 28 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Aluminum magnesium sodium oxide (Al10.33Mg0.67Na1.67O17)
- EC Number:
- 807-888-6
- Cas Number:
- 110621-40-4
- Molecular formula:
- Al10.33Mg0.67Na1.67O17
- IUPAC Name:
- Aluminum magnesium sodium oxide (Al10.33Mg0.67Na1.67O17)
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 10978-52 H_K
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, dry storate
- Stability under test conditions: given
- Solubility and stability of the test substance in the solvent/vehicle: given
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: suspention in ultrapure water
Method
- Target gene:
- his- (S. typhimurium), trp- (E. coli)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 mix
- Test concentrations with justification for top dose:
- First experiment (standard plate test): 0; 33; 100; 333; 1000; 3000 and 6000 μg/plate (top dose was selected based on purity of the test substance)
Second experiment (preincubation test): 0; 10; 33; 100; 333; 1000 and 3000 μg/plate (top dose limited by bacterioxicity) - Vehicle / solvent:
- ultrapure water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- other: With S9 mix: 2-aminoanthracene (2-AA); without S9 mix: N-methyl-N'-nitro-N-nitrosoguianidine (MNNG)
- Details on test system and experimental conditions:
- Standard plate test
• Salmonella typhimurium
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% [w/v] agar + 0.6% [w/v] NaCl) and 10 mL amino acid solution (minimal amino acid solution for the determination of mutants: 0.5 mM histidine + 0.5 mM biotin) were kept in a water bath at about 42 - 45°C, and the remaining components were added in the following order:
0.1 mL test solution or vehicle (negative control)
0.1 mL fresh bacterial culture
0.5 mL S9 mix (with metabolic activation)
or
0.5 mL phosphate buffer (without metabolic activation)
After mixing, the samples were poured onto Minimal glucose agar plates (Moltox Molecular Toxicology, Inc.; Boone, NC 28607; USA) within approx. 30 seconds. After incubation at 37°C for 48 – 72 hours in the dark, the bacterial colonies (his+ revertants) were counted. The colonies were counted using the Sorcerer Image Analysis System with the software program Ames Study Manager (Perceptive Instruments Ltd., Haverhill, UK).
Colonies were counted manually, if precipitation of the test substance hinders the counting using the Image Analysis System.
• Escherichia coli
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% [w/v] agar + 0.6% [w/v] NaCl) and 10 mL amino acid solution (minimal amino acid solution for the determination of mutants: 0.5 mM tryptophan) were kept in a water bath at about 42 - 45°C, and the remaining components were added in the following order:
0.1 mL test solution or vehicle (negative control)
0.1 mL fresh bacterial culture
0.5 mL S9 mix (with metabolic activation)
or
0.5 mL phosphate buffer (without metabolic activation)
After mixing, the samples were poured onto Minimal glucose agar plates (Moltox Molecular Toxicology, Inc.; Boone, NC 28607; USA) within approx. 30 seconds. After incubation at 37°C for 48 – 72 hours in the dark, the bacterial colonies (trp+ revertants) were counted. The colonies were counted using the Sorcerer Image Analysis System with the software program Ames Study Manager (Perceptive Instruments Ltd., Haverhill, UK).
Colonies were counted manually, if precipitation of the test substance hinders the counting using the Image Analysis System.
Preincubation Test
0.1 mL test solution or vehicle, 0.1 mL bacterial suspension and 0.5 mL S9 mix (with metabolic activation) or phosphate buffer (without metabolic activation) were incubated at 37°C for the duration of about 20 minutes using a shaker. Subsequently, 2 mL of soft agar was added and, after mixing, the samples were poured onto the agar plates within approx. 30 seconds.
After incubation at 37°C for 48 – 72 hours in the dark, the bacterial colonies were counted. The colonies were counted using the Sorcerer Image Analysis System with the software program Ames Study Manager (Perceptive Instruments Ltd., Haverhill, UK). Colonies were counted manually, if precipitation of the test substance hindered the counting using the Image Analysis System.
Controls
Negative controls / Vehicle controls
Each experiment included negative controls in order to check for possible contaminants (sterility control) and to determine the spontaneous mutation rate (vehicle control).
• Sterility control
Additional plates were treated with soft agar, S9 mix, buffer, vehicle and the test substance but without the addition of tester strains.
• Vehicle control
The vehicle control with and without S9 mix only contains the vehicle used for the test substance at the same concentration and volume for all tester strains.
Positive controls
The following positive controls were used to check the mutability of the bacteria and the activity of the S9 mix:
with S9 mix
• 2-aminoanthracene (2-AA) (Sigma-Aldrich; 96%)
- 2.5 μg/plate, dissolved in DMSO
- strains: TA 1535, TA 100, TA 1537, TA 98
- 60 μg/plate, dissolved in DMSO
- strain: Escherichia coli WP2 uvrA
without S9 mix
• N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) (Fluka; 97%)
- 5 μg/plate, dissolved in DMSO
- strains: TA 1535, TA 100
• 4-nitro-o-phenylenediamine (NOPD) (Sigma-Aldrich; 98%)
- 10 μg/plate, dissolved in DMSO
- strain: TA 98
• 9-aminoacridine (AAC) (Sigma-Aldrich; 98%)
- 100 μg/plate, dissolved in DMSO
- strain: TA 1537
• 4-nitroquinoline-N-oxide (4-NQO) (Sigma-Aldrich; 98%)
- 5 μg/plate, dissolved in DMSO
- strain: E. coli WP2 uvrA
The stability of the selected positive controls was well-defined under the selected culture conditions, since they were well-established reference mutagens. - Evaluation criteria:
- Mutagenicity
Individual plate counts, the mean number of revertant colonies per plate and the standard deviations were given for all dose groups as well as for the positive and negative (vehicle) controls in all experiments. In general, six doses of the test substance were tested with a maximum of 5 mg/plate, and triplicate plating was used for all test groups at least in the 1st Experiment. Dose selection and evaluation as well as the number of plates used in repeat studies or further experiments were based on the findings of the 1st Experiment.
Toxicity
Toxicity detected by a
• decrease in the number of revertants (factor ≤ 0.6)
• clearing or diminution of the background lawn (= reduced his- or trp- background growth)
was recorded for all test groups both with and without S9 mix in all experiments and indicated in the tables. Single values with a factor ≤ 0.6 were not detected as toxicity in low dose groups.
Solubility
If precipitation of the test material was observed, it would be recorded and indicated in the tables. As long as precipitation did not interfere with the colony scoring, 5 mg/plate was generally selected and analyzed (in cases of nontoxic compounds) as the maximum dose at least in the 1st Experiment even in the case of relatively insoluble test compounds to detect possible mutagenic impurities. Furthermore, doses > 5 mg/plate might also be tested in repeat experiments for further clarification/substantiation.
Results and discussion
Test results
- Key result
- Species / strain:
- bacteria, other: Salmonella TA 1535, TA 100, TA 1537, TA 98, E. coli
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 3000-6000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Standard plate test without metabolic activation:
Strain |
Test group |
Dose ((µg/plate) |
Mean revertants per plate |
Standard deviation |
Factor |
Individual revertant colony counts |
TA 1535 |
Water |
- |
12.3 |
2.3 |
- |
11, 11, 15 |
|
Test item |
33 |
13.3 |
1.2 |
1.1 |
14, 14, 12 |
|
|
100 |
12.0 |
1.7 |
1.0 |
13, 10, 13 |
|
|
333 |
15.0 |
4.6 |
1.2 |
20, 11, 14 |
|
|
1000 |
13.3 |
2.1 |
1.1 |
15, 14, 11 |
|
|
3000 |
7.0 |
1.0 |
0.6 |
7 B, 6 B, 8 B |
|
|
6000 |
6.7 |
1.5 |
0.5 |
7 B, 8 B, 5 B |
|
MNNG |
5.0 |
5152.3 |
224.5 |
417.8 |
5319, 4897, 5241 |
TA 100 |
Water |
- |
103.0 |
12.0 |
- |
91, 103, 115 |
|
Test item |
33 |
126.7 |
6.7 |
1.2 |
125, 134, 121 |
|
|
100 |
117.0 |
13.9 |
1.1 |
124, 101, 126 |
|
|
333 |
98.7 |
11.4 |
1.0 |
108, 102, 86 |
|
|
1000 |
124.7 |
4.7 |
1.2 |
121, 130, 123 |
|
|
3000 |
86.3 |
8.7 |
0.8 |
79 B, 84 B, 96 B |
|
|
6000 |
74.7 |
8.1 |
0.7 |
71 B, 69 B, 84 B |
|
MNNG |
5.0 |
4218.3 |
128.0 |
41.0 |
4347, 4091, 4217 |
TA 1537 |
Water |
- |
11.3 |
2.1 |
- |
13, 9, 12 |
|
Test item |
33 |
9.3 |
3.2 |
0.8 |
8, 7, 13 |
|
|
100 |
9.0 |
1.0 |
0.8 |
10, 8, 9 |
|
|
333 |
7.7 |
2.1 |
0.7 |
7, 6, 10 |
|
|
1000 |
8.7 |
2.1 |
0.8 |
8, 11, 7 |
|
|
3000 |
6.7 |
4.0 |
0.6 |
11 B, 6 B, 3 B |
|
|
6000 |
7.3 |
1.5 |
0.6 |
6 B, 7 B, 9 B |
|
AAC |
100 |
1350.3 |
267.8 |
119.1 |
1274, 1129, 1648 |
TA 98 |
Water |
- |
25.0 |
7.0 |
- |
22, 20, 33 |
|
Test item |
33 |
29.0 |
5.6 |
1.2 |
34, 23, 30 |
|
|
100 |
22.0 |
4.6 |
0.9 |
17, 26, 23 |
|
|
333 |
20.3 |
4.9 |
0.8 |
17, 26, 18 |
|
|
1000 |
21.7 |
5.5 |
0.9 |
27, 22, 16 |
|
|
3000 |
16.7 |
4.2 |
0.7 |
18 B, 12 B, 20 B |
|
|
6000 |
15.7 |
5.0 |
0.6 |
15 B, 21 B, 11 B |
|
NOPD |
10 |
1039.0 |
51.4 |
41.6 |
999, 1097, 1021 |
E. coli |
Water |
- |
29.0 |
10.8 |
- |
38, 32, 17 |
|
Test item |
33 |
33.0 |
7.0 |
1.1 |
25, 38, 36 |
|
|
100 |
33.7 |
3.8 |
1.2 |
31, 38, 32 |
|
|
333 |
27.0 |
1.0 |
0.9 |
26, 28, 27 |
|
|
1000 |
22.3 |
3.1 |
0.8 |
23, 19, 25 |
|
|
3000 |
12.7 |
2.5 |
0.4 |
15 B, 10 B, 13 B |
|
|
6000 |
5.7 |
2.5 |
0.2 |
6 B, 8 B, 3 B |
|
4-NQO |
5 |
1011.7 |
10.3 |
34.9 |
1009, 1003, 1023 |
Standard plate test with metabolic activation:
Strain |
Test group |
Dose(µg/plate) |
Mean revertants per plate |
Standard deviation |
Factor |
Individual revertant colony counts |
TA 1535 |
Water |
- |
10.7 |
6.4 |
- |
8, 18, 6 |
|
Test item |
33 |
12.0 |
1.0 |
1.1 |
12, 13, 11 |
|
|
100 |
10.7 |
2.9 |
1.0 |
14, 9, 9 |
|
|
333 |
12.0 |
2.6 |
1.1 |
15, 11, 10 |
|
|
1000 |
11.3 |
3.2 |
1.1 |
10, 15, 9 |
|
|
3000 |
8.7 |
3.5 |
0.8 |
5 B, 9 B, 12 B |
|
|
6000 |
7.0 |
1.0 |
0.7 |
8 B, 7 B, 6 B |
|
2-AA |
2.5 |
519.7 |
79.8 |
48.7 |
595, 528, 436 |
TA 100 |
Water |
- |
110.3 |
7.6 |
- |
117, 102, 112 |
|
Test item |
33 |
110.7 |
10.7 |
1.0 |
105, 123, 104 |
|
|
100 |
110.3 |
5.1 |
1.0 |
116, 109, 106 |
|
|
333 |
89.0 |
1.0 |
0.8 |
89, 90, 88 |
|
|
1000 |
105.3 |
10.3 |
1.0 |
114, 108, 94 |
|
|
3000 |
102.3 |
12.7 |
0.9 |
88 B, 112 B, 107 B |
|
|
6000 |
105.7 |
7.4 |
1.0 |
103 B, 100 B, 114 B |
|
2-AA |
2.5 |
1233.3 |
24.9 |
11.2 |
1217, 1262, 1221 |
TA 1537 |
Water |
- |
5.7 |
1.5 |
- |
6, 4, 7 |
|
Test item |
33 |
8.0 |
1.7 |
1.4 |
7, 7, 10 |
|
|
100 |
11.0 |
4.4 |
1.9 |
14, 6, 13 |
|
|
333 |
10.0 |
3.6 |
1.8 |
7, 14, 9 |
|
|
1000 |
11.7 |
2.1 |
2.1 |
14, 11, 10 |
|
|
3000 |
4.7 |
0.6 |
0.8 |
5 B, 5 B, 4 B |
|
|
6000 |
4.0 |
2.0 |
0.7 |
6 B, 2 B, 4 B |
|
2-AA |
2.5 |
154.7 |
41.7 |
27.3 |
111, 194, 159 |
TA 98 |
Water |
- |
24.7 |
6.5 |
- |
31, 25, 18 |
|
Test item |
33 |
25.3 |
0.6 |
1.0 |
25, 25, 26 |
|
|
100 |
24.3 |
8.7 |
1.0 |
17, 34, 22 |
|
|
333 |
25.7 |
4.0 |
1.0 |
21, 28, 28 |
|
|
1000 |
26.7 |
6.5 |
1.1 |
20, 27, 33 |
|
|
3000 |
22.0 |
8.5 |
0.9 |
31 B, 21 B, 14 B |
|
|
6000 |
13.3 |
2.1 |
0.5 |
15 B, 14 B, 11 B |
|
2-AA |
2.5 |
2188.0 |
100.5 |
88.7 |
2072, 2250, 2242 |
E. coli |
Water |
- |
23.7 |
6.7 |
- |
28, 27, 16 |
|
Test item |
33 |
16.7 |
2.5 |
0.7 |
19, 14, 17 |
|
|
100 |
17.7 |
4.0 |
0.7 |
22, 14, 17 |
|
|
333 |
25.7 |
2.3 |
1.1 |
27, 23, 27 |
|
|
1000 |
24.7 |
10.5 |
1.0 |
35, 25, 14 |
|
|
3000 |
22.3 |
5.9 |
0.9 |
18 B, 29 B, 20 B |
|
|
6000 |
24.0 |
1.7 |
1.0 |
26 B, 23 B, 23 B |
|
2-AA |
60 |
318.7 |
95.9 |
13.5 |
329, 409, 218 |
Preincubation test without metabolic activation:
Strain |
Test group |
Dose(µg/plate) |
Mean revertants per plate |
Standard deviation |
Factor |
Individual revertant colony counts |
TA 1535 |
Water |
- |
8.3 |
3.2 |
- |
12, 7, 6 |
|
Test item |
10 |
6.3 |
0.6 |
0.8 |
6, 6, 7 |
|
|
33 |
7.0 |
3.6 |
0.8 |
8, 10, 3 |
|
|
100 |
8.3 |
3.8 |
1.0 |
4, 11, 10 |
|
|
333 |
9.7 |
2.1 |
1.2 |
12, 8, 9 |
|
|
1000 |
7.3 |
1.5 |
0.9 |
7, 9, 6 |
|
|
3000 |
6.7 |
2.3 |
0.8 |
8 B, 8 B, 4 B |
|
MNNG |
5.0 |
2337.0 |
121.0 |
280.4 |
2457, 2339, 2215 |
TA 100 |
Water |
- |
93.0 |
11.5 |
- |
84, 89, 106 |
|
Test item |
10 |
100.0 |
14.7 |
1.1 |
117, 92, 91 |
|
|
33 |
108.3 |
6.5 |
1.2 |
108, 115, 102 |
|
|
100 |
100.7 |
6.4 |
1.1 |
98, 108, 96 |
|
|
333 |
78.7 |
9.0 |
0.8 |
70, 78, 88 |
|
|
1000 |
87.0 |
2.0 |
0.9 |
87, 85, 89 |
|
|
3000 |
79.0 |
4.0 |
0.8 |
83 B, 75 B, 79 B |
|
MNNG |
5.0 |
2234.0 |
98.0 |
24.0 |
2145, 2339, 2218 |
TA 1537 |
Water |
- |
6.3 |
1.5 |
- |
6, 8, 5 |
|
Test item |
10 |
5.3 |
1.5 |
0.8 |
4, 5, 7 |
|
|
33 |
6.3 |
2.1 |
1.0 |
8, 7, 4 |
|
|
100 |
7.0 |
1.0 |
1.1 |
7, 6, 8 |
|
|
333 |
5.0 |
1.0 |
0.8 |
4, 6, 5 |
|
|
1000 |
5.3 |
1.2 |
0.8 |
4, 6, 6 |
|
|
3000 |
0.0 |
0.0 |
0.0 |
0 B, 0 B, 0 B |
|
AAC |
100 |
711.0 |
133.1 |
112.3 |
655, 615, 863 |
TA 98 |
Water |
- |
19.3 |
2.5 |
- |
17, 19, 22 |
|
Test item |
10 |
22.0 |
5.2 |
1.1 |
25, 16, 25 |
|
|
33 |
18.7 |
1.2 |
1.0 |
20, 18, 18 |
|
|
100 |
18.3 |
2.3 |
0.9 |
17, 17, 21 |
|
|
333 |
20.0 |
7.2 |
1.0 |
22, 12, 26 |
|
|
1000 |
21.3 |
5.1 |
1.1 |
27, 20, 17 |
|
|
3000 |
23.0 |
10.4 |
1.2 |
28 B, 30 B, 11 B |
|
NOPD |
10 |
834.3 |
47.4 |
43.2 |
817, 798, 888 |
E. coli |
Water |
- |
21.7 |
8.4 |
- |
12, 27, 26 |
|
Test item |
10 |
23.0 |
4.6 |
1.1 |
28, 22, 19 |
|
|
33 |
16.3 |
3.8 |
0.8 |
12, 18, 19 |
|
|
100 |
17.0 |
4.6 |
0.8 |
21, 12, 18 |
|
|
333 |
22.7 |
5.9 |
1.0 |
25, 27, 16 |
|
|
1000 |
20.0 |
7.9 |
0.9 |
17, 14, 29 |
|
|
3000 |
19.7 |
6.0 |
0.9 |
26 B, 19 B, 14 B |
|
4-NQO |
5 |
343.7 |
60.7 |
15.9 |
407, 338, 286 |
Preincubation test with metabolic activation:
Strain |
Test group |
Dose(µg/plate)
|
Mean revertants per plate |
Standard deviation |
Factor |
Individual revertant colony counts |
TA 1535 |
Water |
- |
11.0 |
4.6 |
- |
16, 7, 10 |
|
Test item |
10 |
11.0 |
5.3 |
1.0 |
5, 13, 15 |
|
|
33 |
9.7 |
2.1 |
0.9 |
8, 9, 12 |
|
|
100 |
7.7 |
2.1 |
0.7 |
10, 6, 7 |
|
|
333 |
12.0 |
2.0 |
1.1 |
12, 10, 14 |
|
|
1000 |
11.7 |
3.5 |
1.1 |
12, 15, 8 |
|
|
3000 |
10.3 |
0.6 |
0.9 |
10 B, 11 B, 10 B |
|
2-AA |
2.5 |
155.7 |
27.5 |
14.2 |
124, 174, 169 |
TA 100 |
Water |
- |
70.0 |
8.7 |
- |
65, 80, 65 |
|
Test item |
10 |
81.0 |
8.2 |
1.2 |
74, 79, 90 |
|
|
33 |
85.0 |
7.9 |
1.2 |
91, 76, 88 |
|
|
100 |
80.7 |
5.1 |
1.2 |
85, 82, 75 |
|
|
333 |
86.7 |
3.8 |
1.2 |
91, 85, 84 |
|
|
1000 |
82.0 |
6.0 |
1.2 |
76, 88, 82 |
|
|
3000 |
81.0 |
3.0 |
1.2 |
81 B, 78 B, 84 B |
|
2-AA |
2.5 |
2209.0 |
129.7 |
31.6 |
2061, 2263, 2303 |
TA 1537 |
Water |
- |
6.3 |
2.3 |
- |
5, 5, 9 |
|
Test item |
10 |
7.7 |
3.1 |
1.2 |
11, 7, 5 |
|
|
33 |
8.7 |
4.0 |
1.4 |
8, 13, 5 |
|
|
100 |
5.3 |
2.3 |
0.8 |
4, 8, 4 |
|
|
333 |
7.3 |
1.2 |
1.2 |
6, 8, 8 |
|
|
1000 |
7.7 |
3.1 |
1.2 |
5, 7, 11 |
|
|
3000 |
5.7 |
1.2 |
0.9 |
5 B, 5 B, 7 B |
|
2-AA |
2.5 |
253.7 |
31.0 |
40.1 |
281, 220, 260 |
TA 98 |
Water |
- |
29.7 |
4.2 |
- |
31, 25, 33 |
|
Test item |
10 |
28.0 |
2.0 |
0.9 |
28, 26, 30 |
|
|
33 |
28.3 |
5.5 |
1.0 |
32, 31, 22 |
|
|
100 |
24.0 |
4.4 |
0.8 |
19, 26, 27 |
|
|
333 |
27.0 |
4.6 |
0.9 |
26, 23, 32 |
|
|
1000 |
31.7 |
9.5 |
1.1 |
21, 39, 35 |
|
|
3000 |
36.7 |
3.2 |
1.2 |
33 B, 39 B, 38 B |
|
2-AA |
2.5 |
3096.3 |
66.0 |
104.4 |
3046, 3171, 3072 |
E. coli |
Water |
- |
17.3 |
1.5 |
- |
16, 17, 19 |
|
Test item |
10 |
23.3 |
9.6 |
1.3 |
25, 13, 32 |
|
|
33 |
28.3 |
1.5 |
1.6 |
28, 27, 30 |
|
|
100 |
20.3 |
1.5 |
1.2 |
20, 19, 22 |
|
|
333 |
20.0 |
2.6 |
1.2 |
18, 23, 19 |
|
|
1000 |
25.0 |
7.9 |
1.4 |
16, 31, 28 |
|
|
3000 |
23.7 |
2.5 |
1.4 |
26 B, 24 B, 21 B |
|
2-AA |
60 |
384.0 |
53.0 |
22.2 |
445, 358, 349 |
Applicant's summary and conclusion
- Conclusions:
- Under the experimental conditions chosen here, it is concluded that ß’’-Alumina (BASF) is not a mutagenic test substance in the bacterial reverse mutation test in the absence and the presence of metabolic activation.
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