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EC number: 410-610-2 | CAS number: 111850-24-9 MORTRACE SB CONC.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Although the study methodology followed was as per OECD TG 202 and EU Method C2 and in accordance with the Principles of Good Laboratory Practices (GLP), analytical analyses of the tested concentrations was not performed.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- no
- Details on sampling:
- - Range finding test concentrations: WAF's prepared at 10, 100 and 1000 mg/l
- Main study test concentrations: WAF's prepared at 10, 18, 32, 56 and 100 mg/l - Vehicle:
- yes
- Details on test solutions:
- The procedure for preparation of the exposure solutions was as follows: weighed amounts (19.2, 36.7, 64.7, 55.6 and 97.9 mg) of test substance were spread out on microscopic slides and these amounts corresponded to the nominal concentrations to be tested. These microscopic slides with thetest material were melted in a water bath at a temperature of ca. 60°C. (the first 3 slides with the test substance were put in 2 liters of ISO-medium, the last two in 1-litre of ISO medium).
Each slide with the test substance was put in the required volume of ISO-medium, which was subsequently stirred for ca. 91 hours, untl the start of the test. After stirring, the microscopic slides were removed and the solutions were filtered through a paper filter to remove little drops of the test substance that had come off from the slides. The final test solutions were clear without precipitation and the intensity of the resulting water accomodated fractions (WAF's) increased with concentrations. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: magna
- Source: in-house
- Age at study initiation: < 24 hours
- Method of breeding: With new born daphnids, i.e. < 3 days old by placing about 250 of these into 10 litres of medium in glass culture vessels.
- Maximum age of the cultures: 4 weeks
- Renewal of the cultures: after 7 days of cultivation, half of the medium twice a week
- Feeding during test: no
- Food type during breeding: daily, a suspension of fresh water algae
- Temperature of medium: 18-22 °C, constant within ± 1 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- no information
- Test temperature:
- 20 - 23 °C
- pH:
- ranged between 8.0 - 8.3
- Dissolved oxygen:
- ranged between 8.7 - 10.0 mg/l
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Sampling for analysis of actual expoure concentrations at the end of the test was cancelled as samples taken during the fish toxicity test revealed only detectable amounts of test substance in the WAF's of 1000 mg/l (nominal).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml glass vessel
- Aeration: no aeration
- No. of organisms per vessel: 20/test concentration
- Loading: 10 per vessel containing 100 ml medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium (as per standard recommendations)
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED: Immobilization was noted
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: WAF's prepared at 10, 100 and 1000 mg/l
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32 - 56 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- other: water accomodated fraction
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% fiducial limits could not be calculated using the Finney model as the index of regression significance was > 1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- other: water accomodated fraction
- Basis for effect:
- mobility
- Remarks on result:
- other: The 48-hour EC50 corresponded with a WAF prepared at 45 mg/l with 95% fiducial limits of WAF's prepared at 42.3 mg/l and 49.8 mg/l.
- Details on results:
- Range finding test- Immobility was observed in all the daphnids exposed to the WAF's prepared at 100 and 1000 mg/l. No immobility was seen in the WAF prepared at 10 mg/l.
Main study - After 24 hours of exposure the total rate of immobilization was 55% in both the WAF's prepared at 56 and 100 mg/l. No immobilization of the daphnids was noted in the WAF's prepared at 32 mg/l or lower concentrations. Some of the daphnids present in the WAF's prepared at WAF's prepared at 32 mg/l or higher were found trapped at the surface and were re-immersed.
After 48 hours of exposure immobilization of all the daphnids were noted in the WAF prepared at 100 mg/l. The total incidence of immobility among the daphids exposed to the WAF prepared at 56 mg/l had increased to 90% and no significant (>10%) immobilization was seen at WAF's prepared at 32 mg/l or lower.
The pH varied between 8.0 - 8.3 and oxygen concentrations were found to be > 8 mg/l for all measurements and the temperature varied between 20 - 23 °C. In the controls, no daphnia became immobilized or trapped at the surface of the water.
The 24-hour EC50 corresponded with a WAF prepared between 32 and 56 mg/l and 95% fiducial limits could not be calculated using the Finney model as the index of regression significance was > 1. The 48-hour EC50 corresponded with a WAF prepared at 45 mg/l with 95% fiducial limits of WAF's prepared at 42.3 mg/l and 49.8 mg/l. The NOEC with respect to mobility of daphnia was found to correspond with a WAF prepared at a concentration of 32 mg/l. - Results with reference substance (positive control):
- The 24-hour EC50 was 0.57 mg/l (95% fiducial limits: 0.48 - 0.73 mg/l) and the 48-hour EC50 was 0.40 mg/l (95% fiducial limits: 0.35 - 0.49 mg/l).
- Reported statistics and error estimates:
- standard statistical methods were employed
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study Mortarce SB Conc. did not induce acute immobilization of daphnia magna exposed to WAF prepared at a concentration of 32 mg/l or lower. The 48-hour EC50 corresponded with a WAF prepared at 45 mg/l with 95% fiducial limits of WAF's prepared at 42.3 mg/l and 49.8 mg/l. The NOEC with respect to mobility of daphnia was found to correspond with a WAF prepared at a concentration of 32 mg/l.
- Executive summary:
In this study, daphnids were exposed for a maximum of 48 hours to a range of water accomodated fractions (WAF's ) prepared at 10, 18, 32, 56 and 100 mg/l. Weighed amounts of the test substance were spread out on microscopic slides and placed in the test media, which were stirred for ca. 91 hours until the start of the exposure. After stirring, the slides were removed and the solutions were filtered through a filter paper. The final test solutions were all clear and light brown with the intensity of the colour increasing with concentration. The test was perfomed in duplicate with 10 daphnids per vessel.
Under the conditions of the study Mortarce SB Conc. did not induce acute immobilization of daphnia magna exposed to WAF prepared at a concentration of 32 mg/l or lower. The 48-hour EC50 corresponded with a WAF prepared at 45 mg/l with 95% fiducial limits of WAF's prepared at 42.3 mg/l and 49.8 mg/l. The NOEC with respect to mobility of daphnia was found to correspond with a WAF prepared at a concentration of 32 mg/l.
Reference
None
Description of key information
In an acute toxicity study with Daphnia magna, the 48-hour EC50 value for Mortrace SB Conc. corresponded with a water accommodated fraction of 45 mg/L. Actual measurement of Mortrace SB Conc. were not undertaken in this study. However, the concentrations of Mortrace SB Conc. in the water fraction corresponding to the 48-hour EC50 are expected to be similar to those determined in the fish acute toxicity study (i.e. 10 μg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10 µg/L
Additional information
One static acute toxicity test with Daphnia magna was available for Mortrace SB Conc. This study was assigned a Klimisch score of 2 based on the lack of analytical measures of test solution concentrations. The lack of measured concentrations in this study was based on minimal detection in the fish acute toxicity test with Mortrace SB Conc. The 48-hour EC50 value (based on immobility) was reported as 45 mg Mortrace SB Conc/L. This value was based on initial nominal loading rate of the water accommodated fraction, however the measured water concentration of Mortrace SB Conc.at this loading rate are expected to be much lower, likely in the range of 10 μg/L as was seen in the fish acute toxicity study. Thus, the key parameter for the acute invertebrate endpoint was the EC50 value of 45 mg/L, which, based on the acute toxicity test with the fish, was assumed to be approximately 10 μg/L in the water column.
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