Alfalfa, ext.

Vapour pressure

Link to relevant study record(s)

Reference

Endpoint:

vapour pressure

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From May to June 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Reliability 1

Qualifier:

according to guideline

Guideline:

EU Method A.4 (Vapour Pressure)

Deviations:

no

GLP compliance:

no

Type of method:

static method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: AL01
- Expiration date of the lot/batch: 10.09.2019
- Purity test date: not available

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: refrigerated (2 to 8°C)
- Stability under test conditions: not available
- Solubility and stability of the test substance in the solvent/vehicle: not applicable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not applicable

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: not applicable
- Final preparation of a solid: not applicable

OTHER SPECIFICS: none

Key result

Temp.:

20 °C

Vapour pressure:

425 Pa

Full test results

Temp/°C	Temp / K	P/mbar	**P/Pa
Run 1
*20	293.15	4.47	447
20.0	293.15	4.4	440
30.0	303.15	9.4	940
39.9	313.05	17.9	1790
50.0	323.15	35.6	3560
60.0	333.15	61.1	6110
Run 2
*20	293.15	4.2	420
20.0	293.15	4.1	410
30.0	303.15	8.7	870
40.2	313.05	17.3	1730
50.0	323.15	29.6	2960

 *Determined by extrapolation

**Calculated

Comment

Run 1: 12.46g of sample used. Sample was degassed for 19 hours. Vacuum at beginning of analysis was 0.004mbar.

Run 2: 12.68g of sample used. Sample degassed for 19 hours. Vacuum at start of testing was 0.0041 mbar.

Conclusions:

In a static method test, conducted according to EU Regulation (EC) 440/2008, Annex Part A test A4, a vapor pressure of 425 Pa was determined for Alfalfa, ext. at 20°C.

Executive summary:

A static method, conducted according to EU Regulation (EC) 440/2008, Annex Part A test A4, was performed to determine the vapor pressure of Alfalfa, ext.

Alfalfa, ext. was placed in the glass chamber connected to a vacuum system linked with pressure monitors enabling the reading of any pressure/ vacuum of the contents of the static glassware. A suitable manometer liquid was placed in U-tube. The system was evacuated when the optimum vacuum was observed. The glassware was then isolated from the vacuum system and positioned in the constant temperature bath set to a desired starting temperature. As the static apparatus and its contents approached temperature equilibrium in the bath, the levels of the manometer liquid were balanced with pressure. This procedure was repeated until the system reached temperature and pressure equilibrium (i.e., no significant change in temperature or pressure were observed). The temperature and pressure displayed on the pressure monitors were then recorded. The temperature was subsequently set to a higher temperature and the procedure was repeated until enough data points were obtained.

 A vapor pressure of 425 Pa was determined for Alfalfa, ext. at 20°C.

Description of key information

Vapor pressure: 425 Pa at 20°C

Key value for chemical safety assessment

Vapour pressure:

425 Pa

at the temperature of:

20 °C

Additional information

A static method, conducted according to EU Regulation (EC) 440/2008, Annex Part A test A4, was performed to determine the vapor pressure of Alfalfa, ext.

The vapor pressure of 425 Pa was determined for Alfalfa, ext. at 20°C