2-[(3-aminopropyl)methylamino]ethanol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-12-13 to 2007-04-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

results presented are based on nominal concentrations, no analytical verification of test concentrations performed

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Test Item Name: 2-[(3-Aminopropyl) methylamino]ethanol
- Batch Number: PFW060026
- Label Identification: XTA-758
- Expiration date of the lot/batch: not provided
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage Conditions: Ambient
- Stability under test conditions: no data
- Solubility and stability of the test substance in the solvent/vehicle: no data

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The five concentrations of the test substance used in the definitive test were 25, 50, 100, 200 and 400 mg/L. The flasks were set on a shaker table for constant agitation at ~ 100 cycles/minute.
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no data

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: freshwater algae
- Source (laboratory, culture collection): STILLMEDADOW, Inc. cultures
- Age of inoculum (at test initiation): mass culture initiated 3-7 ± 1 days prior to dosing
- Method of cultivation: Parents and daughter cultures are continuously maintained at STILLMEADOW, Inc. Three days prior to testing, 1000 mL of autoclaved algal stock culture medium (Woods Hole) was inoculated. This new mass culture was then placed in a ~25°C environmental chamber and maintained on continuous aeration. All procedures were conducted aseptically.

ACCLIMATION
- Acclimation period: not relevant

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not reported

Test temperature:

24 ± 2°C

pH:

pH at the start (after 0 hours): 7.4 for the control and 10.4 for 400 mg/L
pH at the end (after 96h): 7.1-8.6

Dissolved oxygen:

not applicable

Salinity:

not relevant

Conductivity:

not relevant

Nominal and measured concentrations:

Range-finder:
nominal test concentrations: 1, 10, 100, 500 and 1000 mg/L

Final test:
nominal test concentrations : 25, 50, 100, 200 and 400 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass sterile Erlenmeyer flasks
- Type: with stoppers and aeration tubing
- Material, size, headspace, fill volume: 250 mL all-glass flasks
- Initial cells density: 10,000 cells/mL
- Control end cells density: 4,77 x 10E+06 cells/mL (average accross 3 replicates)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes, algal stock culture medium (Woods Hole) with EDTA up to 0.300 mg/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Algal stock culture medium (Woods Hole) with EDTA up to 0.300 mg/L
- Culture medium different from test medium: no
- Intervals of water quality measurement:
* temperature: continuously
* pH: at the beginning in all concentrations and at the end of the test in the control and the limit concentration.

OTHER TEST CONDITIONS
- Sterile test conditions: yes, all procedures were conducted aseptically
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 400 ± 40 ft.c.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Cell densities were determined using a hemocytometer.
- effect calculated parameters: specific growth rate

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10, 100, 500 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes.

Reference substance (positive control):

yes

Remarks:

Zinc Chloride

Key result

Duration:

96 h

Dose descriptor:

IC50

Effect conc.:

127.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% C.L.: 0.00-186.58 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- 96-h LOEC = 25 mg/L
- 96-h EC10 (extrapolated) = 7.85 mg/L

Reported statistics and error estimates:

The NOEC and IC50 were determined by a commercially available statistical program (ToxCalc Version 5.0 TM). Several models are available for IC50 determination: Probit, Trimmed Speatman-Karber, and Linear Interpolation. The most appropriate model was selected for the determination in this case.

Validity criteria fulfilled:

yes

Conclusions:

A 96-h growth inhibition test with the unicellular green alga Selenastrum capricornutum was performed with the test substance according to the approved protocol SOPs and OPPTS, series 850, section 5400. The test concentration of the test item that resulted in 50% inhibition (IC50) was determined to be 127.10 mg/L with 95% C.L. of 0.00-186.58 mg/L. As the NOEC was determined to be <25 mg/L (the lowest tested concentration), the test item may be hazardous to aquatic algae.. The results of the test can be considered reliable without restrictions.

Description of key information

The study by Rodrigue (2007), investigating the acute toxicity of the test substance to algae according to the approved protocol SOPs and OPPTS, series 850, section 5400, was considered as the key study for endpoint coverage. The 72-h NOEC and 72-h IC50 for growth rate were determined to be < 25 mg/L (the lowest tested concentration) and 127.1 mg/L (> 100 mg/L) respectively. As the NOEC was determined to be <25 mg/L (the lowest tested concentration),  the test item may be hazardous to aquatic algae. The results of the test can be considered reliable without restrictions.

Key value for chemical safety assessment

EC50 for freshwater algae:

127.1 mg/L

EC10 or NOEC for freshwater algae:

7.85 mg/L