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Diss Factsheets
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EC number: 458-680-3 | CAS number: 797751-44-1 WASOX-VMAC2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22-25 February, 2005.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- According to EU Method C.1, with GLP.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
0.5003 g test substance was dispersed in 5000 mL dilution water. The test substance was added in drops to approximately 1000 mL dilution water, stirred by using magnetic stir plates and bars. Then the required amount of dilution water was added. The preparation was stirred for approximately half an hour to obtain a preparation for testing in which the test substance is completely hydrolyzed.
The concentration is presented in units of mg test substance equivalents / L to indicate that not the test substance but a variety of degradation products was tested.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Brachydanio rerio
- Source: Fa. Amazonas aquarium, Grimmenstein.
- Feeding during test: no feeding (last feeding about 24 hours before start of the exposure).
ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Feed: TetraMin; Tetra Werke, Dr.rer.nat. Ulrich Baensch GmbH, D-49304 Melle.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No.
- Hardness:
- Total hardness: 250 mg CaCO3/L
- Test temperature:
- 20 ºC throughout the test period
- pH:
- Between 7.6 -8.1 in control medium, and between 7.8-8.0 in the test substance medium
- Dissolved oxygen:
- Test substance medium: 90-94%, and control medium: 90-97%.
- Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: full glass vessel (47 cm x 29 cm x 31 cm), filled with ca. 40 L, covered with a transparent plastic lid.
- Aeration: continuous aeration by compressed air and additional aeration by water recirculation.
- No. of organisms per vessel: 7
OTHER TEST CONDITIONS
- Photoperiod: artificial light from 4 a.m. to 8 p.m.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
All animals were observed for alterations and behavioural changes at setting-in of the fish and 2, 4, 24, 48, 72 and 96 hours afterwards. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Mortality
Number of fish at the start of exposure: 7 in each group.
The nominal concentration of test substance equivalents is displayed.
Group
ConcentrationTotal number of dead fish
at hours after start of exposure(mg/L)
0
24
48
72
96
Control
0
0
0
0
0
0
Test substance
100
0
0
0
0
0
There were no unforeseen events.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hours LC50 value of the test substance, based on the nominal concentration of test substance equivalents at the start of the assay, was >100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
The concentrations are presented in units of mg test substance equivalents / L to indicate that not the test substance but a variety of degradation products was tested. - Executive summary:
The Acute Toxicity of the test substance to the Zebrafish (Brachydanio rerio) was determined in a 96 hour static test (According to EU Method C.1). A total of 7 fish were treated with a single dose of 100 mg/L (Limit test). A negative control group was included. Test validity criteria were fulfilled.
The 96-hours LC50 value of the test substance, based on the nominal concentration at the start of the assay, was > 100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
Reference
Description of key information
The 96-hours LC50 value of the test substance, based on the nominal concentration at the start of the assay, was > 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The Acute Toxicity of the test substance to the Zebrafish (Brachydanio rerio) was determined in a 96 hour static test (According to EU Method C.1). A total of 7 fish were treated with a single dose of 100 mg/L (limit test). The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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