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EC number: 260-086-2 | CAS number: 56271-94-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13/07/2018 to 30/07/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- other: cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties
- Specific details on test material used for the study:
- Identification:
OK (640-2) (Furoxy Hydroxy)
CAS number: 56271-94-4
Appearance/Physical state: Cream colored solid
Batch: G317624
Purity: 94.905%
Retest date: 05 September 2018
Storage conditions: Approximately 4 °C in the dark - Radiolabelling:
- no
- Test temperature:
- 25 °C
- Details on study design: HPLC method:
- Test System
The determination was carried out by the HPLC screening method, using a procedure
designed to be compatible with Method 121 of the OECD Guidelines for Testing of
Chemicals, 22 January 2001.
The test system utilized a high performance liquid chromatograph. A commercially available
cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
Performance of the Test
Preparation of sample solution
Test item (0.1003 g) was diluted to 100 mL with methanol. This solution was further diluted
as follows: 10 mL, plus 45 mL of purified water, and then diluted to 100 mL with methanol,
to give a concentration of 100 mg/L.
Preparation of dead time solution
The dead time was determined by measuring the retention time of formamide (purity
99.6%) of a 602 mg/L solution in mobile phase.
Preparation of reference standard solutions
Solutions of reference standards (see table below) were prepared in methanol.
Determination of retention time
The sample, dead time and reference standard solutions were injected in duplicate using the
following HPLC parameters:
HPLC System : Agilent Technologies 1200, incorporating workstation and autosampler
Detector type : variable wavelength (VW)
Column : Waters XSelect HSS Cyano 5µm (150 x 4.6 mm id)
Column temperature : 25 °C
Mobile phase : Methanol: pH 7 buffer solution* (55:45 v/v)
pH of mobile phase : 7.0
Flow-rate : 1.0 mL/min
Injection volume : 10 µL
VW detector wavelength : 210 nm (dead time and reference standards), 275 nm (sample)
* Specification: Disodium hydrogen orthophosphate anhydrous (3 mM), Potassium dihydrogen
orthophosphate (2 mM), Sodium chloride (2 mM)
Evaluation of Data
Capacity factor
The capacity factor for each reference standard was calculated using the following equation:
Equation 1:
k' = (tr-t0)/t0
where k' = capacity factor; tr = retention time (min); t0 = deadtime
Construction of the calibration curve
A correlation of log k' versus log Koc of the reference standards was plotted using linear
regression (Appendix 2). The capacity factors (k') for the reference standards were calculated
using the previous equation. Log10Koc values are those quoted in Method 121 of the OECD
Guidelines for Testing of Chemicals, 22 January 2001.
Adsorption coefficient
Initially the capacity factor of the test item was calculated using Equation 1. The adsorption
coefficient was then calculated using the following equation with reference to the calibration
curve (Appendix 2).
Equation 2:
log10 Koc = log10k' -A/B
where Koc= absorption coefficient; k' = capacity factor; A = intercept of calibration curve; B = slope of calibration curve
4.1.3.4 Classification
The mobility classification of the test item was obtained by comparing the calculated Koc
value to the mobility classes according to P.J. McCall, R.L. Swann, D.A. Laskowski,
S.M. Unger, S.A. Vrona and H.J. Dishburger “Estimation of Chemical Mobility in Soil from
Liquid Chromatography Retention Times”; Bull. Environm. Contam. Toxicol. 24, 190-195
(1980) as shown in the table below: - Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- < 1.25 dimensionless
- pH:
- 7
- Temp.:
- 25 °C
- Key result
- Sample No.:
- #2
- Type:
- log Koc
- Value:
- < 1.25 dimensionless
- pH:
- 7
- Temp.:
- 25 °C
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: see table below
- Details of fitted regression line (log k' vs. log Koc): log k'= 0.3495 (logKow) - 0.7789 r2= 0.9438
- Graph of regression line attached- yes
- Average retention data for test substance: retention time 1.506 min; capacity factor <0.376; log10k' <-0.425; log10Koc<1.25 - Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of the test item has been determined to be less than 17.8,
log10Koc < 1.25. The results classify the test item as having a very high mobility. - Executive summary:
The adsorption coefficient of OK (640-2) (Furoxy Hydroxy) has been determined to be less
than 17.8, log10Koc< 1.25, using the HPLC screening method, designed to be compatible
with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001. The
results classify the test item as having a very high mobility.
Reference
System Deadtime
Dead time |
Retention Times (min) |
Mean Retention Time |
|
Injection 1 |
Injection 2 |
||
Formamide |
1.973 |
1.973 |
1.973 |
Retention times, capacity factors and absorption coefficients of reference standards
Standard |
Retention time (min) |
Mean Retention Time (min) |
Capacity factor (k’) |
Log10 k’ |
Log10 Kow |
|
Injection1 |
Injection2 |
|||||
Acetanilide |
2.716 |
2.711 |
2.714 |
0.376 |
-0.425 |
1.25 |
Phenol |
2.57 |
2.57 |
2.57 |
0.303 |
-0.519 |
1.32 |
Atrazine |
3.785 |
3.785 |
3.785 |
0.919 |
-0.037 |
1.81 |
Linuron |
5.51 |
5.51 |
5.51 |
1.793 |
0.254 |
2.59 |
Naphthalene |
4.752 |
4.752 |
4.752 |
1.409 |
0.149 |
2.75 |
Endosulfan-diol |
6.686 |
6.706 |
6.696 |
2.394 |
0.379 |
3.02 |
Fenthion |
8.314 |
8.29 |
8.302 |
3.208 |
0.506 |
3.31 |
a-Endosulfan |
11.453 |
11.497 |
11.475 |
4.817 |
0.683 |
4.09 |
Diclofop-methyl |
12.726 |
12.658 |
12.692 |
5.433 |
0.735 |
4.2 |
Phenanthrene |
8.912 |
8.989 |
8.95 |
3.537 |
0.549 |
4.09 |
DDT |
24.869 |
24.747 |
24.808 |
11.575 |
1.064 |
5.63 |
Description of key information
The adsorption coefficient of OK (640-2) (Furoxy Hydroxy) has been determined to be less
than 17.8, log10Koc< 1.25, using the HPLC screening method, designed to be compatible
with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001. The
results classify the test item as having a very high mobility.
Key value for chemical safety assessment
- Koc at 20 °C:
- 17.8
Additional information
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