Benzoic acid, 5-[(4-aminophenyl)azo]-2-hydroxy-, reaction products with 3-[(4-amino-3-methoxyphenyl)azo]benzenesulfonic acid and carbonic dichloride, sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13.12.2002 – 18.07.2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Test No. L 43 "Fish, Acute Toxicity test. Chemical formulations and waste waters."

Deviations:

no

Principles of method if other than guideline:

- Principle of test:A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. A sample during the test formed a fine precipitate that gradually settled at the bottom. The total volume for one dilution is 1000 ml in preliminary test and 4000 ml in limit test.- Short description of test conditions:The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.Test duration 96 hours, the temperature of the solutions 21.0 ± 1.0 °C.- Parameters analysed / observed: mortality at 48 and 96 h

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM- Common name: Guppy- Strain: Poecilia reticulata- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč- Age at study initiation (mean and range, SD): 3 - 4 monts- Length at study initiation (length definition, mean, range and SD): 2 - 3 cmACCLIMATION- Acclimation period: yes- Acclimation conditions (same as test or not): same as testFEEDING DURING TESTno feeding during testfasted 72 hours before test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.0 ± 1.0 °C

pH:

from 7.84 (0 hour) to 7.65 (96 hour)control: 7.88 (0 hour) to 7.50 (96 hour)

Dissolved oxygen:

from 8.3 (0 hour) to 7.8 mg/L O2 (96 hour)control: from 8.3 (0 hour) to 7.8 mg/L

Nominal and measured concentrations:

100 mg.L-1

Details on test conditions:

TEST SYSTEM- Material, size, headspace, fill volume: open 10 L glass aquarium- No. of organisms per vessel: Preliminary test: 3 animals Limit test: 10 animals - No. of vessels per concentration/control (replicates): no replicatesTEST MEDIUM / WATER PARAMETERSDilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled waterA) 117.6 g CaCl3.2H2O in 1L of distilled waterB) 49.3 g MgSO4.7H2O in 1L of distilled waterC) 25.9 g NaHCO3 in 1L of distilled waterD) 2.3 g KCl in 1L of distilled waterThe dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C. EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h TEST CONCENTRATIONS - Range finding study: 100, 75, 50, 25 mg.L-1 - Test concentration: 100 mg.L-1 - Results used to determine the conditions for the definitive study: no mortality in preliminary test

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Executive summary:

The test substance, Saturnová žluť L4G 75 (Direct Yellow 44), was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 43 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Description of key information

96 h - LC50 > 100.0 mg.L-1

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L