Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 424-660-8 | CAS number: -
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 - 14 April 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: done under GLP and OECD method
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Method C1, Directive 92/69/EEC
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 424-660-8
- EC Name:
- -
- Cas Number:
- 224631-15-6
- Molecular formula:
- Hill formula: C18H26N4O5S CAS formula: C18H26N4O5S
- IUPAC Name:
- 2,5-dioxopyrrolidin-1-yl (2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the beginning and then at 96hrs for the 22, 48 and 100 % concentrations. Also ran a stability sample concurrent to look for maintenace of test solution concentration.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 100 mg/l solution of A-133816.0
2.5 g of A-133816.0 to test tanks of 25 litres of charcoaled filterd water.
Tank contents ultrasonicated for 3-4 min.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Rainbow trout of (Oncorhynchus mykiss) of length 4-6 cm
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Fish observed at 1, 3, 6 and 24 h and thereafter daily up to 96 h
Test conditions
- Hardness:
- 68 mg CaCO3/l
- Test temperature:
- 14.7-15.2 oC
- pH:
- missing
- Nominal and measured concentrations:
- nomimal concentration = 100 mg/l A-133816.0
- Details on test conditions:
- light cycle of 16 h light and 8 h dark
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- LC 50 and NOEC are both greater than 100 mg/l
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 for the test substance is estimated to be greater than 100 mg/l
The NOEC for the test sustance is estimated to be greater than 100 mg/l
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
