Dimethyl(octyl)amine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 March 1996 - 07 August 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Guideline not mentioned in report but test methodology is in accordance with OECD 203.

Deviations:

not specified

GLP compliance:

no

Remarks:

Study conducted in accordance with principles of GLP but no formal quality assurance procedures were undertaken.

Specific details on test material used for the study:

Test item referred to as Farmin DM08P in report, this is a trade name for N,N-dimethyloctan-1-amine (AD-1)

Analytical monitoring:

no

Remarks:

Performed at nominal concentrations

Vehicle:

no

Details on test solutions:

0, 0.1 mg/L; 0.32 mg/L; 1.0 mg/L; 3.2 mg/L; 10 mg/L; 32 mg/L; and 100 mg/L

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The rainbow trout used in the test were supplied by Wilmington Trout Fann, Devon and arrived at Huntingdon Life Sciences (Eye) 19 January 1996. At the laboratory, they were held in an aerated supply of dilution water under flow-through conditions until removed for testing.
The fish were maintained and the study conducted in treated tap water (dechlorinated tap water which had been blended with reverse-osmosis, softened tap water). No mortality occurred during the 14-day period before the test. The mean wet-weight of the fish used in the test was 1.2 g; their mean fork length was 4.8 cm.

Test type:

static

Water media type:

other: treated tap water (dechlorinated tap water which had been blended with reverse-osmosis, softened tap water).

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

n/a

Hardness:

220-246 mg/L CaCO3

Test temperature:

13.2-14.6 °C

pH:

8.0-8.6

Dissolved oxygen:

75-107 % ASV

Salinity:

not measured

Conductivity:

not measured

Nominal and measured concentrations:

0, 0.1 mg/L; 0.32 mg/L; 1.0 mg/L; 3.2 mg/L; 10 mg/L; 32 mg/L; and 100 mg/L

Details on test conditions:

Groups of five fish were exposed to FARMIN DM 08P at nominal concentrations of 0.1, 0.32, 1, 3.2, 10, 32 and 100 mg/L. A control group comprising five fish was exposed to dilution water alone.
At 1 mg/L and higher concentrations, the test media were prepared by adding the appropriate weight of test material directly to the dilution water (14 litres); at 0.1 and 0.32 mg/L, they were prepared by dilution of an aqueous stock solution nominally containing 10 mg/L. At 32 and 100 mg/L, the pH of the dilution medium was affected by the test material and required adjustment with 1M hydrochloric acid; their pHs were adjusted from 8.5 and 8.9 respectively to 8.3 before use. The media were gently aerated during the test.
The results of the water quality analysis conducted at the start of the test and at 24-hour intervals thereafter.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.7 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on non-linear interpolation between the two concentrations which bracket 50% effect level (3.2 and 10 mg/L)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Reported statistics and error estimates:

n/a

Validity criteria fulfilled:

not specified

Conclusions:

Screening test was performed using nominal test concentrations 0.1 mg/L; 0.32 mg/L; 1.0 mg/L; 3.2 mg/L; 10 mg/L; 32 mg/L; and 100 mg/L over 96 hour period.
The LC50 concentration for AD-1 for rainbow trout was estimated to be 5.7 mg/L

Executive summary:

A study was conducted to determine the acute toxicity of the test substance AD-1 to Onchoryncus mykiss (rainbow trout).

 

Range finding tests were performed to determine the approximate response range for the test species. Nominal concentrations used in this test included 0.0 mg/L (control), 0.1 mg/L; 0.32 mg/L; 1.0 mg/L; 3.2 mg/L; 10 mg/L; 32 mg/L; and 100 mg/L. Bioassays with trout consisted of a single exposure containing a total of five fish per test level. The results of this preliminary test showed that AD-1 was lethal to trout concentrations > 3.2 mg/L and < 10 mg/L.

Based on non-linear interpolation between the two concentrations which bracket 50% effect level (3.2 and 10 mg/L), the LC50 was estimated at 5.7 mg/L. The NOEC concentration was 0.1 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.7 mg/L