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EC number: 278-115-2 | CAS number: 75199-13-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 September to 12 September, 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- - Preparation: the sample was maintained at 21 °C with continuous aeration and used on the day of collection. The pH was buffered to pH 7.1 with sodium hydroxide solution and the suspended solids level adjusted to 4.1 g/l prior to use.
- Test organisms (species):
- activated sludge of a predominantly industrial sewage
- Details on inoculum:
- - Inoculum: a mixed population of activated sewage sludge microorganisms
- Source: the aeration stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire
- Method of preparation: direct dispersion in water - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
- Test temperature:
- Room temperature
- pH:
- 7.1
- Nominal and measured concentrations:
- Measured concentrations are not specified. Nominal concentrations are used for all evaluations.
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration: 3 replicates
- No. of vessels per control: 2 replicates
- Adjustment of pH: buffered to pH 7.1 with sodium hydroxide solution
TEST CONCENTRATIONS
- Concentrations used in range finding study : 10, 100 and 1000 mg/l
- Results used to determine the conditions for the definitive study: percentage inhibition
- Definitive test concentrations: 1000 mg/l (3 replicates; limit test) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- - Percentage inhibition of test item: 0-7 % (3 replicates)
- Results with reference substance (positive control):
- - Percentage inhibition of reference item: 12-79 % (2 replicates)
- 3 hour EC50 = 12 mg/l - Conclusions:
- The 3 hour EC50 of the test item to microorganisms is greater than 1000 mg/l (nominal).
- Executive summary:
The potential for the test item to inhibit activated sludge respiration was evaluated in an experimental study according to a method comparable to the OECD guideline 209. In a range finding study, a mixed population of activated sewage sludge microorganisms exposed to test item concentrations of 10, 100 and 1000 mg/l for 3 hours inhibited oxygen consumption by -9 % (increase), 12 % and 14 %, respectively, compared to two replicates of a negative control. A reference substance (3,5-dichlorophenol: 3.2 and 32 mg/l) were tested in parallel which inhibited oxygen consumption by 14 % and 84 % at 3.2 and 32 mg/l, respectively. Based on these findings, a limit test was performed in the definitive study using the highest concentration.
In the definitive study, three replicates of 1000 mg/l were not found to inhibit oxygen consumption compared to two replicates of the negative control: oxygen consumption was inhibited by the test item by 0 %, 0 % and -7 % (increase). Based on this finding, the 3 hour EC50 of the test item was found to be greater than 1000 mg/l.
Oxygen consumption of the reference substance was inhibited by 12 % and 79 % at concentrations of 3.2 and 32 mg/l, respectively, therefore the 3 hour EC50 of the reference substance was found to be 12 mg/l. Analytical determinations of test item concentration were not performed during the study, therefore, all findings are based on the nominal test item concentrations.
Reference
Table 1: Results from the range finding study.
Substance | Concentration (mg/l) | O2 consumption rates (mg/O2/min) 3 hour contact time | % inhibition |
Control | 0.0 0.0 |
0.36 0.41 |
- - |
Test item | 10 100 1000 |
0.42 0.34 0.33 |
<9> 12 14 |
Reference item | 3.2 32 |
0.33 0.06 |
14 84 |
<increase>
Variation between controls: ± 6 %
Table 2: Results from the definitive study.
Substance | Concentration (mg/l) | O2 consumption rates (mg/O2/min) 3 hour contact time | % inhibition |
Control | 0.0 0.0 |
0.42 0.42 |
- - |
Test item | 1000 1000 1000 |
0.42 0.42 0.45 |
0 0 <7> |
Reference item | 3.2 32 |
0.37 0.09 |
12 79 |
<increase>
Variation between controls: zero
Description of key information
EC50 (3 h) > 1000 mg/l (nominal)
Key value for chemical safety assessment
Additional information
Toxicity of the substance to microorganisms was examined in a 3 hour exposure to the test item in an activated sludge respiration inhibition limit test. 1000 mg/l test item was not found to inhibit oxygen consumption, therefore, the concentration which reduced oxygen consumption by 50 % (EC50) after 3 hours of exposure is greater than 1000 mg/l, reported as nominal concentration due to poor water solubility.
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