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EC number: 244-584-7 | CAS number: 21799-87-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 8.9.2000 to 24.10.2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: Test method of new chemical substance
- Version / remarks:
- Kanpogyo No. 5, Yakuhatsu No. 615 and 49 Kikyoku No. 392 dated 13th July 1974
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsuboshi Chemicals, Japan, KCL2287
- Expiration date of the lot/batch:
- Purity test date:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Refrigerated storage
- Stability under test conditions: IR spectrum before and after the experiment are collated and thus it was confirmed the stability of test substanceunder the storage condition.
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Returned sludge from sewage treatment and Surface soil at water's edge contacting surface water and atmosphere.
- Laboratory culture:
- Method of cultivation: Approximately 30 minutes after stop the aeration into the cultivating vessel, approximately 1/3 of total supernatant liquid was removed. De-chlorinated water was added to the vessel to make total volume 10 L and re-aerated for more than 30 minutes and, artificial sewage of 50 g/L was added to the vessel to adjust concentration of artificial sewage to 0.1 wt% in the added de-chlorinated water. The same procedure was repeated once a day and activated sludge was provided after cultivating.
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge: 30 mg/l
- Initial cell/biomass concentration: no data
- Water filtered: yes
- Type and size of filter used, if any: - Duration of test (contact time):
- >= 14 d
- Initial conc.:
- >= 100 mg/L
- Based on:
- not specified
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- >= 91
- Sampling time:
- 14 d
- Parameter:
- other: BOD
- Value:
- >= 102
- Sampling time:
- 14 d
- Parameter:
- other: HPLC
- Value:
- >= 100
- Sampling time:
- 14 d
- Results with reference substance:
- It was confirmed that the test condition was valid, because the degree of degradation of aniline on Day7 and 14 determined by BOD was 75% and 76%, respectively
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The study shows that the degradation by BOD is 102%, result by TOC is 91% and result by HPLC is 100%.
Reference
Analytical result of test liquid on Day 14
System & Vessel No. |
Water + Test substance |
Sludge + Test substance |
Theoretical value |
|||||
1 |
2 |
3 |
4 |
|||||
BOD* |
mg |
0 |
26 |
26.7 |
24.3 |
25.2 |
||
* DOC residual amount and rate |
mgC |
8.9 |
0.7 |
0.9 |
0.7 |
9.5 |
||
% |
94 |
7 |
10 |
8 |
- |
|||
Residual amount of test substance and residual rate (HPLC) |
mg |
29.6 |
0 |
0 |
0 |
30.0 |
||
% (1) |
99 |
0 |
0 |
0 |
- |
|||
*Value of (Sludge + Test substance) system was shown after deduction of value of blank system.
Description of key information
The study shows that the degradation by BOD is 102%, result by TOC is 91% and result by HPLC is 100%.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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