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EC number: 249-033-4 | CAS number: 28462-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Remarks:
- source of read across
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Similar Substance 02
- IUPAC Name:
- Similar Substance 02
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: animals were purchased from KFM, CH-4414 Fülllinsdorf, Switzerland.
- Age at study initiation: young adult, age not specified.
- Weight at study initiation: ranged between 140 and 180 g.
- Fasting period before study: access of food only was prevented approximately 18 hours prior and four hours after the dosing. The water bottles were withdrawn two hours prior and four hours after dosing.
- Housing: housed individualry in Macroron cages.
- Diet: standard laboratory pelleted diet (KLIBA no. 24-343-7 from Klingentatmühle AG, Basle). The batch of diet used for the study was analysed for chernical and microbiological contaminants.
- Water: ad libitum.
- Acclimation period: animals were acclimatised to the experimental environment for a period of about five days prior to the start of the study.
ENVIRONMENTAL CONDITIONS
- Temperature: 23 ± 2 °C
- Humidity: 50 ± 10 %
- Air changes: approximately 15 changes/hour.
- Photoperiod: the light/dark cycle was 12 hours.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- DMSO
- Details on oral exposure:
- - Test fomulation: test item was dissolved in DMSO; the test sample was prepared immediately before dosing.
- Dose volume: 15 mI per kg body weight. - Doses:
- 5350 mg/kg bw, higher dosages were not applicable.
- No. of animals per sex per dose:
- Five male and five female.
- Details on study design:
- - Duration of observation period following administration: the animals, on both the preliminary and the main studies, were observed for 14 days after dosing.
- Frequency of observations: animals were observed soon after dosing, then at hourly intervals for the remainder day 1. On the subsequent days the animals were observed once in the morning and once in the late afternoon. Clinical signs were recorded at each observation.
- Frequency of weighing: individual body weights were recorded on day 1, 7 and 14.
- Necropsy of survivors performed: surviving animals were killed after two weeks. All animals which died during the study and those killed after two weeks were subjected to a macroscopic postmortem examination. The macroscopic appearance of the abnormal organs was recorded.
PRELIMINARY TEST
A preliminary study was carried out to establish a dosing regimen, using groups of two males and two females at two dosage, using 10-15 ml/kg body weight. - Statistics:
- The acute oral LD5O was determined using the probit method of L.C. Miller and M.L. Tainter (Proc. Soc. exper. Biol. Med. 57 (1944) p. 26) or calculated by computer using the method of D.J. Finney (3rd. ed., 1971). The given doses aiways referred to the test material.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 350 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- No death occurred during the range-finding experiment.
During the main experiment, one female died 59 hours after dosing. - Clinical signs:
- No overt symptoms of poisoning were observed.
- Gross pathology:
- No special findings could be detected.
Any other information on results incl. tables
Mortality ratio and group mean bodyweight (g) of rats
Sex | Dosage | Bodyweight (g) at | Mortality ratio | ||
Dosing | 1 wk | 2wks | |||
Male | Test item, 5350 mg/kg bw | 177 | 207 | 246 | 0/5 |
Female | Test item, 5350 mg/kg bw | 166 | 163 | 183 | 1/5 |
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified, according to CLP Regulation (EC 1272/2008)
- Conclusions:
- LD50 > 5350 mg/kg bw (males and females)
- Executive summary:
Test item was dissolved in DMSO; the test sample was prepared immediately before dosing. A preliminary study was carried out to establish a dosing regimen, using groups of two males and two females at two dosage, using 10-15 ml/kg body weight. In the main experiment, groups of five male and five female rats were dosed at levels selected using the results of the preliminary study. The animals were dosed by oral gavage by means of a stomach tube and the dosed volume was 15 ml per kg body weight. The animals on both, the preliminary and the main studies were observed for 14 days after dosing.
Surviving animals were killed after two weeks. All animals which died during the study and those killed after two weeks were subjected to a macroscopic postmortem examination. The macroscopic appearance of the abnormal organs was recorded.
One female died 59 hours after dosing. No overt symptoms of poisoning were observed. No special findings could be detected.
Conclusion
LD50 > 5350 mg/kg bw (males and females)
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