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EC number: 240-851-7 | CAS number: 16830-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017/12/20 to 2018/03/29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate
- EC Number:
- 240-851-7
- EC Name:
- O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate
- Cas Number:
- 16830-15-2
- Molecular formula:
- C48H78O19
- IUPAC Name:
- [6-[[3,4-dihydroxy-6-(hydroxymethyl)-5-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl] 10,11-dihydroxy-9-(hydroxymethyl)-1,2,6a,6b,9,12a-hexamethyl-2,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydro-1H-picene-4a-carboxylate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch 170430040156
Manufacturing date 15 February 2017
Expiry date 15 February 2020
Supplier SEPPIC
Appearance White powder
Packaging Plastic bag
Date of receipt 16 November 2017
Storage In darkness at room temperature
Identification number of the test item 17-300
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- The inoculum was prepared from activated sludge sampled in the aeration tank treatment plant receiving predominantly domestic sewage (Montataire - (60) France). After setting and washing, activated sludge was used as inoculum.
Activated sludge collected on: 18 January 2018
- Dry matter of activated sludge: 3.1 g/L
- Concentration of inoculum:
- Dry matter: 0.9 g/L
- Approximately 107 - 108 cells/L
- Organic carbon content: 85.3 mg/L
- Turbidity: 120 NTU - Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 52.9 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test system:
-Respirometric OxiTop® measuring system: the measuring system consists of reaction vessels (12 test flasks of 500 mL), each with a CO2 absorber, a magnetic stirring and a measuring head (the measuring heads record and save the pressure difference semi-continuously throughout the experiment).
-Vessel: cylindrical glass vessel of 500 mL capacity containing 305 mL of test medium (except toxicity control: 215 mL).
-Inoculum: prepared from activated sludge receiving predominantly domestic sewage, not pre-adapted to the test item.
-Test item: containing the test item “O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate” at the concentration of 52.9 mg/L in inoculated medium – 4 vessels;
-Controls: - blank control (inoculated medium) – 2 vessels
- procedure control: reference item to check the activity of the inoculum – 1 vessel
- toxicity control: to check any inhibitory effect of the compound (test item and reference item) – 2 vessels
- abiotic sterile control: to check any abiotic degradation (sterilising agent) – 2 vessels
- sterile control: test medium and sterilising agent – 1 vessel
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 75.1
- St. dev.:
- 8.6
- Sampling time:
- 28 d
- Details on results:
- VALIDITY CRITERIA
- Percentage biodegradation of reference item (Aniline) in control flasks FC was 80.2% of ThOD within 14-day period (OECD 301 F pass level = 60% within 14-day period).
- The percentage of biodegradation in the test vessel F Inh. was 84.3% of ThOD after 14 days of incubation. The test item is not assumed to be inhibitory towards inoculum at this concentration according the OECD 301 F pass level.
- For the test item, the difference between the extreme degradation values was less than 20% at the end of the test.
- The oxygen uptakes of inoculum blanks at the end of the test were less than 60 mg/L medium.
- The pH values were 7.3 at the end of the experiment for test item flasks.
All the criteria are fulfilled, the test is considered as valid.
Any other information on results incl. tables
% biodegradation (ThOD) after 28 days:
Vessels | Test item: O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate | Reference item: Aniline | |||
FE1 52.9 mg/L | FE2 52.9 mg/L | FE3 52.9 mg/L | FE4 52.9 mg/L | FC 41.6 mg/L | |
% ThOD | 81.0% | 80.2% | 76.7% | 62.6% | 100% |
Mean: 75.1% | / |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The average level of biodegradation achieved for the test item is: 75.1% of ThOD after 28 days of incubation and 60% of ThOD has been reached in a 10-d window, criteria for good evidence of “ready biodegradability” were reached under test conditions (i.e. the pass level for ready biodegradability is 60% of ThOD and this value has to be reached in a 10-day window within the 28-day of the test).
So, the test item “O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate” can be considered as readily biodegradable, according to the OECD Guideline 301 for the testing of chemicals. - Executive summary:
Test purpose
Evaluation, in an aqueous mineral medium inoculated with microbial flora from activated sludge, of the ready biodegradability of the test item “O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate”, according to the test procedure described in the OECD Guideline for the testing of chemicals 301 F, in conformity with the clauses recommended in the study plan of 20 December 2017, signed by the sponsor representative on 04 January 2018.Test method
OECD Guideline for the testing of chemicals 301 F: Manometric respirometry (17 July 1992)
Test item
O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate
Test concentration
52.9 mg/L (100 mg ThODNH3)
Reference item
Aniline, 41.6 mg/L (100 mg ThODNH3)
Inoculum
Prepared from activated sludge from a treatment plant receiving predominantly domestic sewage.
Duration
28 days of incubation
Results
- % biodegradation (ThOD) after 28 days:
Vessels
Test item: O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate
Reference item: Aniline
FE1
52.9 mg/L
FE2
52.9 mg/L
FE3
52.9 mg/L
FE4
52.9 mg/L
FC
41.6 mg/L
% ThOD
81.0%
80.2%
76.7%
62.6%
100%
Mean: 75.1%
/
Conclusion
The average level of biodegradation achieved for the test item is: 75.1% of ThODafter 28 days of incubation and 60% of ThODhas been reached in a 10-d window,criteria for good evidence of “ready biodegradability” were reached under test conditions (i.e. the pass level for ready biodegradability is 60% of ThOD and this value has to be reached in a 10-day window within the 28-day of the test).
So, the test item “O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl (2α,3β,4α)-2,3,23-trihydroxyurs-12-en-28-oate” can be considered as readily biodegradable, according to the OECD Guideline 301 for the testing ofchemicals.
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