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EC number: 275-602-1 | CAS number: 71550-21-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC, UV/VIS
- Details on sampling:
- Control at 96 h
test concentrations at 0 h and 96 h - Vehicle:
- no
- Details on test solutions:
- Pre-treatment of test item and preparation of test item concentrations
A stock solution was prepared to give the desired limit test concentration of 100 mg/L active ingredient:
- 558 mg of Bayscript Yellow GGN ( = 500 mg/L active ingredient) were added to 5 litres of dilution water
- treated for 1 h in an ultrasonic bath and stirred for 1 h on a magnetic stirrer. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Name: Zebra fish (Danio rerio)
Source: Interaquaristik.de Shop (Germany)
Date of birth: --
Date supplied: 2016-08-24
Acclimatisation: Stock held since 2016-08-24 and acclimatised to the test conditions since then.
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
Mortalities during acclimatisation period: < 5 %
Medication: none
Mean standard length (n = 14): 3.75 cm (S.D. = 0.24 cm) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 14.2 °dH (=253.5 mg/L CaCO3)
- Test temperature:
- 21.9 - 22.7 °C
- pH:
- 7.5 - 7.9
- Dissolved oxygen:
- 8.2 - 9.1
- Nominal and measured concentrations:
- nominal (mg/L): 100
measured (mg/L): 98.702 (0 h), 99.714 (96 h) - Details on test conditions:
- Exposure conditions
Test vessels: glass aquaria holding 5 L of test media covered by glass plates
Experimental design: 1 test concentration plus 1 control, 7 fish per test concentration, no feeding during the exposure period, static system
Method of initiation: fish were placed in prepared media
Loading: 1.62 g body weight (wet weight) per litre
Photoperiod: 16 h light: 8 h dark
Temperature: 21.9 to 22.7 °C
Aeration: gentle aeration via narrow glass tubes
Test item concentration: 100 mg/L active ingredient
Method of administration: direct weighing
Medium renewal: none
Duration of exposure: 96 hours
Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed. - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of nominal concentrations. Effective concentrations correspond to 98.7 % of nominal values at 0 hours and to 99.7 % of nominal values at 96 hours.
As no toxic effects against fish were observed, no statistical analysis was required to determine the LC50. - Validity criteria fulfilled:
- yes
- Remarks:
- mortality in the controls did not exceed 10 % by the end of the test; dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit
- Conclusions:
- After 96 h a LC50 > 100 mg/L (active ingredient) was determined for Bayscript Yellow GGN in a static test toward fish (Danio rerio). All results are expressed in terms of nominal concentrations.
- Executive summary:
A study was performed to assess the acute toxicity of Bayscript Yellow GGN to Danio rerio under static conditions in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish was exposed to a limit test concentration of nominally 100 mg/L of the active ingredient of Bayscript Yellow GGN dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath and a magnetic stirrer.
During the test a temperature range of 20 - 24 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h of exposure. After 96 h a LC50 > 100 mg/L (active ingredient) was determined.
No toxic effects against fish were observed at a limit test concentration of 100 mg/L of the active ingredient of the test item.
All results are expressed in terms of nominal concentrations. Effective concentrations correspond to 98.7 % of nominal values at 0 hours and to 99.7 % of nominal values at 96 hours.
Reference
Description of key information
After 96 h a LC50 > 100 mg/L (active ingredient) was determined for Bayscript gelb GGN in a static test toward fish (Danio rerio). All results are expressed in terms of nominal concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Should read: LC50 > 100 mg/L.
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