N-dodecyl-β-alanine, compound with 2,2',2''-nitrilotriethanol (1:1)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

Data refers to sodium salt.
Triethanolamine is not classified as hazarodus in the environment and the sodium salt is considered a suitable surrogate for read-across

Principles of method if other than guideline:

No supporting analysis was performed. No all data endpoints are reported.

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Substance fully soluble in water

Test organisms (species):

Chlorella vulgaris

Details on test organisms:

Culture type CCAP 211/12
Source : Culture Collection of Algae and Protozoa
Maintained 20 C, shaking 200 rpm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

No applicable

Hardness:

50 mg/l CO3

Test temperature:

23 C +/-2

pH:

7.9 at start, rising to 9.7 in controls and sequentionally lower to 7.6 in highest tested concentration of 100 mg/l

Dissolved oxygen:

Not reported

Salinity:

Not applicable

Nominal and measured concentrations:

No analysis, so nominal only reported
Concentrations are expressed in terms of the material tested. The material tested is ASSUMED 30% ACTIVES.
6 mg/l as supplied, estimate 1.8 mg/l actives
13 mg/l as supplied, estimate 3.9 mg/l actives
25 mg/l as supplied, estimate 7.6 mg/l actives
50 mg/l as supplied, estimate 15.1 mg/l actives
100 mg/l as supplied, estimate 30 mg/l actives

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

ca. 2 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

ca. 4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

ca. 3 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

ca. 5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 9.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Details on results:

Graphical summary of result attached as pdf.

Concentration actives, assuming 30% solids	Actives	Inhibition 48 hr		Inhibition 72 hr
	mg/l	% (rate)	% (biomass)	% (rate)	% (biomass)
	0	0	0	0	0
	1.8	6	22	0	0
	3.9	39	78	2	21
	7.6	58	93	24	80
	15.1	100	100	100	100
	30	100	100	100	100

Validity criteria fulfilled:

yes

Remarks:

Valid study, despite no analysis

Conclusions:

Considered toxic for the aquatic environment, with GHS classification as Aquatic Acute 2

Executive summary:

The study was performed in 1995 using valid OECD methodology and performed in accordance to GLP requirements.

However, the report does not fully evaluate the data and the EC10 and NOEC have been derived based on tabulated data. There was no supporting analysis performed, although the substance is known to be stable.

Supported by the acute fish and acute Daphnia reports [and the 2012 Daphnia reproduction study], the substance appears to be toxic to aquatic organisms.

It is predicted that the triethanolamine salt will have a lower toxicity in view of the low biological effect of triethanolamine and the molecular weight difference between triethanolamine and sodium; however, due to uncetainty factors in read-across, it is assumed that the toxicity of the triethanolamine salt will be equivalent to that of the sodium salt.

Description of key information

The effect of biomass was greater than the effect on growth rates over the 72 hour test period.

The ErC50 and EbC50 values are in the same order of magnitude and either can be used for classification purposes.

The results based on growth rates are cited below.

Data refers to sodium salt.

Triethanolamine is not classified as hazarodus in the environment and the sodium salt is considered a suitable surrogate for read-across

No further animal testing can be justified.

Key value for chemical safety assessment

EC50 for freshwater algae:

9.4 mg/L

EC10 or NOEC for freshwater algae:

5.5 mg/L

Additional information

The study was performed in 1995 using valid OECD methodology and performed in accordance to GLP requirements.

However, the report does not fully evaluate the data and the EC10 and NOEC have been derived based on tabulated data. There was no supporting analysis performed, although the substance is known to be stable.