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EC number: 619-220-9 | CAS number: 96499-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Feb - 21 Feb 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- March 23, 2006; Annex 5 corrected July 28, 2011
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dodecanoic acid, monoester with oxybis[propanediol]
- EC Number:
- 619-220-9
- Cas Number:
- 96499-68-2
- Molecular formula:
- not applicable, the substance is UVCB
- IUPAC Name:
- Dodecanoic acid, monoester with oxybis[propanediol]
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling: Samples for analysis were taken from all test concentration levels at the start of exposure, at 24 and 48 h and at the end of exposure.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0585 g of the test item and 1300 mL of the test medium were mixed to prepare a stock solution with a nominal loading rate of 45.0 mg/L and stirred for 48 h. The test solutions were prepared by dilution of the stock solution with the appropriate amount of test medium.
- Eluate: no
- Differential loading: no
- Controls: blank control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Freshwater green algae
- Strain: ATCC22662
- Source: American Type Culture Collection, supplied on 30 June, 1995
- Method of cultivation: sterile conditions; temperature: 21-24 °C; light intensity: nominal 6923 lux; continuous illumination provided by alternative LED light to fluorescent lamp
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.1 - 22.5 °C
- pH:
- 7.8 - 7.9
- Nominal and measured concentrations:
- nominal: Control, 1.00, 3.16, 10.0, 31.6 and 100 mg/L
measured: < detection limit, 0.310, 0.979, 3.02, 10.1 and 39.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterilised 300 mL Erlenmeyer flasks with gas-permeable Silicosen
- Initial cells density: 0.75 x 10E04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: same as test medium
- Intervals of water quality measurement: The pH value was measured at test start and end. The temperature was measured at test start, after 24 and 48 h and at test end.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous light
- Light intensity and quality: 6769-7077 lux in wavelength range 400 - 700 nm; continuous illumination provided by alternative LED light to fluorescent light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Biomass was measured every 24 hours after test start using chlorophyll fluorescence measurement (spectrofluorometer model RF-6000, Shimadzu).
- Chlorophyll measurement: yes, using a spectrofluorometer
- Other: Cell conditions were evaluated microscopically for each test concentration level at the end of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: Two preliminary tests were performed.
Study 1: Nominal concentrations of 10.0 and 100 mg/L were chosen. Growth rate inhibition was 5.8% at the low concentration and > 100% at the high concentration.
Study 2: Nominal concentrations of 2.00, 16.0 and 50.0 mg/L were chosen. Growth rate inhibition was 1.0% at the lowest concentration, 31% at the intermediate concentration and > 100% at the highest concentration. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 12.4-14.9 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.02 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): At 10.1 and 39.6 mg/L, some bloated cells were observed. Cells of other treatment solutions were the same as the control. For the control cells, the cell condition was normal.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: At test start and end, the test solution of 39.6 mg/L was colourless and clear with some bubbles. All other test solutions were colourless and clear at test start. At test end all other test solutions, except 39.6 mg/L, showed green colour due to algal growth. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- ErC50 (72 h) = 1.3 mg/L - Reported statistics and error estimates:
- Estimation of EC50: The percentage inhibition in each exposure level was plotted as a semi-logarithmic graph against the corresponding concentration. Linear regression analysis (least square method) was carried out using the data within the range showing linearity visually (100 and 31.6% levels) to estimate the EC50 and 95% confidence limits, which were estimated from the intercept of the regression line with the parallel drawn to the absciss at 50% inhibition. The EC50 was denoted as ErC50 based on growth rate.
Estimation of NOEC: Regarding growth rate, Bartlett's test was done to determine the homogeneity of variance for the data. Then, one-way analysis of variance (ANOVA) and Dunnett's multiple comparison test was used to determine the significant difference between the control and exposure levels. Concentration levels above the ErC50 were excluded for each analysis.
Any other information on results incl. tables
Table 1: Biomass values
Values are based on the measured value of the pre-culture.
Measured concentration [mg/L] |
No. |
Chlorophyll fluorescence value (relative value) |
|||
0 h |
24 h |
48 h |
72 h |
||
Control |
A |
43 |
200 |
950 |
3800 |
B |
43 |
180 |
870 |
3500 |
|
C |
43 |
190 |
840 |
3600 |
|
D |
43 |
180 |
860 |
3700 |
|
E |
43 |
190 |
830 |
3400 |
|
F |
43 |
190 |
830 |
3200 |
|
Mean |
43 |
190 |
860 |
3500 |
|
SD |
0 |
7.0 |
44 |
230 |
|
0.310 |
A |
43 |
200 |
980 |
4100 |
B |
43 |
180 |
900 |
3500 |
|
C |
43 |
190 |
850 |
3300 |
|
Mean |
43 |
190 |
910 |
3600 |
|
SD |
0 |
11 |
64 |
440 |
|
0.979 |
A |
43 |
210 |
980 |
3600 |
B |
43 |
200 |
920 |
3500 |
|
C |
43 |
190 |
780 |
3300 |
|
Mean |
43 |
200 |
890 |
3500 |
|
SD |
0 |
12 |
100 |
150 |
|
3.02 |
A |
43 |
210 |
930 |
3700 |
B |
43 |
190 |
830 |
3500 |
|
C |
43 |
200 |
950 |
3700 |
|
Mean |
43 |
200 |
900 |
3600 |
|
SD |
0 |
10 |
65 |
130 |
|
10.1 |
A |
43 |
110 |
400 |
1500 |
B |
43 |
130 |
430 |
1500 |
|
C |
43 |
130 |
460 |
1700 |
|
Mean |
43 |
120 |
430 |
1600 |
|
SD |
0 |
9.9 |
31 |
100 |
|
39.6 |
A |
43 |
14 |
1.2 |
1.6 |
B |
43 |
13 |
2.0 |
2.0 |
|
C |
43 |
13 |
2.7 |
3.9 |
|
Mean |
43 |
13 |
2.0 |
2.5 |
|
SD |
0 |
0.59 |
0.73 |
1.3 |
Table 2: Growth rate and growth inhibition rate
Measured concentration [mg/L] |
No. |
Growth rate 0-72 h |
Growth inhibition rate [%] |
Control |
A |
1.49 |
- |
B |
1.47 |
- |
|
C |
1.48 |
- |
|
D |
1.49 |
- |
|
E |
1.46 |
- |
|
F |
1.43 |
- |
|
Mean |
1.47 |
- |
|
SD |
0.0226 |
- |
|
0.310 |
A |
1.53 |
-3.8 |
B |
1.47 |
0.16 |
|
C |
1.45 |
1.2 |
|
Mean |
1.48 |
-0.82 |
|
SD |
0.0393 |
2.7 |
|
0.979 |
A |
1.48 |
-0.54 |
B |
1.47 |
0.14 |
|
C |
1.45 |
1.4 |
|
Mean |
1.46 |
0.34 |
|
SD |
0.0147 |
1.0 |
|
3.02 |
A |
1.49 |
-1.3 |
B |
1.47 |
0.18 |
|
C |
1.49 |
-1.1 |
|
Mean |
1.48 |
-0.74 |
|
SD |
0.0118 |
0.80 |
|
10.1 |
A |
1.19 |
19 |
B |
1.20 |
19 |
|
C |
1.23 |
16 |
|
Mean |
1.21 |
18 |
|
SD |
0.0209 |
1.4 |
|
39.6 |
A |
1.10 |
175 |
B |
-1.03 |
170 |
|
C |
-0.797 |
154 |
|
Mean |
-0.977 |
166 |
|
SD |
0.160 |
11 |
Table 3: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
factor of 74 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
4.5% |
yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
1.5% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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