Reaction mass of 2-(3-{6-[3-(2-Hydroxyethyl)-3-methylureido]hexyl}-1-methylureido)ethanol and 6-[3-(2-Hydroxyethyl)-3-methylureido]hexylamino 3-(methylamino)propionate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Version / remarks:

1981, 00372 Version 1

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Date of receipt : 2014-07-18
Expiry date : 2016-01-07

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge from an aeration tank of two different wastewater treatment plants treating predominantly domestic wastewater (Wupper area water authority, WWTP Odenthal and WWTP Cologne-Stammheim).
Activated sludge from the aeration tanks of a wastewater treatment plant treating predominantly wastewater of industrial origin (WWTP Leverkusen Bürrig).
The three sludge types were mixed taking 2 parts from each of the two domestic WWTPS plus 1 part from the industrial WWTP.
Date of collections : 2015-08-17

Duration of test (contact time):

28 d

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

Consumption of oxygen (BOD) and chemical oxygen demand (COD)

Details on study design:

A measured volume of inoculated mineral medium, containing a known concentration of 30 mg/L of the test item was stirred in a closed flask at a constant temperature (25 ± 2 °C) under aerobic conditions in the dark.
The consumption of oxygen (BOD) was determined by measuring the drop in pressure in the automated respirometer flasks. Evolved carbon dioxide was absorbed in sodium hydroxide. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of the chemical oxygen demand (COD).
The test lasted for 28 days.
The endogenous activity of the inoculum was checked running parallel blanks with inoculum but without test item. A reference compound (sodium benzoate) was run in parallel to check the operation of the procedures.
A toxicity control (test item and reference compound mixed, one replicate) was run in parallel, to ensure that the chosen concentration of the test item was not inhibitory to microorganisms.
Because of the nature of biodegradation and of the mixed bacterial populations used as inoculum, determinations of test item were carried out in quadruplicate, for the inoculum blank in triplicate and for the reference compound in duplicate.
The oxygen uptake was calculated from the readings taken at regular and frequent intervals, using the method given by the manufacturer of the equipment. At the end of incubation, the pH was measured in the flasks.
The degradation rate of the test item was set in relation to its experimentally determined COD. As the COD implicitly covers the oxygen demand for the nitrification process, there was no need to take additional correction measures into account.
Start of the incubation period : 2015-08-20
End of the incubation period : 2015-09-17

Reference substance:

benzoic acid, sodium salt

Remarks:

From Acros Organics, Purity: 99.9 %, Expiry date: 2018-03-27

Parameter:

% degradation (O2 consumption)

Value:

14

Sampling time:

7 d

Remarks on result:

other: Degradation mean from analysis in four flasks

Parameter:

% degradation (O2 consumption)

Value:

19

Sampling time:

14 d

Remarks on result:

other: Degradation mean from analysis in four flasks

Parameter:

% degradation (O2 consumption)

Value:

23

Sampling time:

21 d

Remarks on result:

other: Degradation mean from analysis in four flasks

Key result

Parameter:

% degradation (O2 consumption)

Value:

23

Sampling time:

28 d

Remarks on result:

other: Degradation mean from analysis in four flasks

Key result

Parameter:

BOD5

Value:

11.6 other: mg O2/L

Remarks on result:

other: blank-corrected mean BOD after 28d

Parameter:

COD

Value:

1.651 other: mg O2/mg

Remarks on result:

other: Degradation of toxicity control, COD analytically determined

Results with reference substance:

The reference compound sodium benzoate showed 81 % degradation after 7 days and 82 % degradation after 14 days. Theoretical oxygen demand of reference compound (test concentration of 30 mg/L): 1.665 mg O2/mg.

Validity criteria fulfilled:

yes

Remarks:

The percent degradation of the reference compound sodium benzoate reached the level of ≥ 40 % after 7 days and ≥ 65 % after 14 days. No toxicity of the test item was observed in the toxicity control.

Interpretation of results:

not inherently biodegradable

Conclusions:

Within 28 days a degradation rate of 23 % was determined for EGGE 2806-1 and therefore EGGE 2806-1 is considered to be "Not Inherently Biodegradable".

Executive summary:

This study was performed to assess the inherent biodegradability of EGGE 2806-1. The study was conducted in accordance with the OECD Guideline 302C “Inherent Biodegradability: Modified MITI Test (II)” (1981).

A suspension of EGGE 2806-1 in a mineral medium was inoculated with 40 % sludge of each of two different municipal sewage treatment plants (STP) and 20 % sludge of an industrial STP and incubated under aerobic conditions. During this period, degradation was followed by continuous automated BOD determinations.

EGGE 2806-1 showed:

14 % degradation after 7 days

19 % degradation after 14 days

23 % degradation after 21 days

23 % degradation after 28 days

Therefore, EGGE 2806-1 is considered to be “Not Inherently Biodegradable”.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

Adopted July 1992, 00090 Version 1

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Date of receipt : 2014-07-18
Expiry date : 2015-07-12

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Origin : aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, WWTP Odenthal)
Date of collection : 2015-05-18
Concentration of inoculum : 30 mg/L suspended solids

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

Consumption of oxygen (BOD) and chemical oxygen demand (COD)

Details on study design:

A suspension of 100 mg/L test item in a mineral medium, equalling to 50-100 mg ThOD or COD/Litre as the nominal sole source of organic carbon, was stirred in a closed flask and inoculated at a constant temperature (22 ± 1 °C) for up to 28 days under aerobic conditions in the dark.
The consumption of oxygen (BOD) was determined by measuring the drop in pressure in the automated respirometer flasks. Evolved carbon dioxide was absorbed in sodium hydroxide. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of chemical oxygen demand (COD).
The endogenous activity of the inoculum was checked running parallel blanks with inoculum but without test item. A reference compound (sodium benzoate) was run in parallel to check the operation of the procedures.
A toxicity control (test item and reference compound mixed, one replicate) was run in parallel, to ensure that the chosen concentration of the test item was not inhibitory to microorganisms.
Degradation was followed by the determination of oxygen uptake and measurements were taken at frequent intervals to allow the identification of the beginning and end of biodegradation and the slope of the biodegradation curve.
The test lasted for 28 days.
Because of the nature of biodegradation and of the mixed bacterial populations used as inoculum, determinations of test item and inoculum blank were carried out in triplicate and of reference compound in duplicate.
The oxygen uptake was calculated from the readings taken at regular and frequent intervals, using the method given by the manufacturer of the equipment. At the end of incubation, the pH was measured in the flasks.
The degradation rate of the test item was set in relation to its experimentally determined COD. As the COD implicitly covers the oxygen demand for the nitrification process, there was no need to take additional correction measures into account.
Start of the incubation period : 2015-05-20
End of the incubation period : 2015-06-17

Reference substance:

benzoic acid, sodium salt

Remarks:

from Acros Organics, Purity: 99.9 %, Expiry date: 2018-03-27

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

7 d

Remarks on result:

other: Degradation mean from analysis in three flasks

Parameter:

% degradation (O2 consumption)

Value:

10

Sampling time:

14 d

Remarks on result:

other: Degradation mean from analysis in three flasks

Parameter:

% degradation (O2 consumption)

Value:

16

Sampling time:

21 d

Remarks on result:

other: Degradation mean from analysis in three flasks

Key result

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

28 d

Remarks on result:

other: Degradation mean from analysis in three flasks

Key result

Parameter:

BOD5

Value:

29 other: mg O2/L

Remarks on result:

other: blank-corrected mean BOD after 28d

Parameter:

COD

Value:

1.651 other: mg O2/mg

Remarks on result:

other: Degradation of test item, COD analytically determined

Results with reference substance:

The reference compound sodium benzoate showed 87 % degradation after 14 days.

All validity criteria of the test method were met:

The reference compound reached the level of ≥ 60 percent for ready biodegradability within 14 days.

The toxicity control exhibited degradation rates > 25 % within 14 days.

At the end of the test, at the plateau, or the end of the 10-d window, biodegradation in parallels with test item did not differ by more than 20 percentage points.

The oxygen uptake of the inoculum blank was ≤ 60 mg/L.

In cases where the degradation was ≤ 60 %, the pH values were in between 6.0 and 8.5 at the end of the test.

Validity criteria fulfilled:

yes

Remarks:

All validity criteria of the test method were met

Interpretation of results:

not readily biodegradable

Conclusions:

Within 28 days, a degradation rate of 18 % was determined for EGGE 2806-1. EGGE 2806-1 is considered to be "Not Readily Biodegradable".

Executive summary:

This study was performed to assess the ready biodegradability of EGGE 2806-1. It was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.4-D “Manometric Respirometry Test“(2008). This test method is in all essential parts identical with OECD Guideline 301 F (adopted July 1992).

A suspension of EGGE 2806-1 in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by continuous automated BOD determinations.

EGGE 2806-1 showed:

1 % degradation after 7 days

10 % degradation after 14 days

16 % degradation after 21 days

18 % degradation after 28 days

Therefore, EGGE 2806-1 is considered to be “Not Readily Biodegradable“.

Description of key information

A study was performed to assess the inherent biodegradability of EGGE 2806-1. The study was conducted in accordance with the OECD Guideline 302C “Inherent Biodegradability: Modified MITI Test (II)” (1981).

EGGE 2806-1 showed 23 % degradation after 28 days and is therefore considered to be “Not Inherently Biodegradable”.

A further study was performed to assess the ready biodegradability of EGGE 2806-1. The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.4-D “Manometric Respirometry Test“(2008). This test method is in all essential parts identical with OECD Guideline 301 F (adopted July 1992).

EGGE 2806-1 showed 18 % degradation after 28 days and is therefore considered to be “Not Readily Biodegradable“.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed