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EC number: 916-461-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Organisms: a mixed population of activated sewage sludge micro-organisms.
- Source: the aeration stage of Severn Trent Water PLC Sewage Treatment Plant, Belper, Derbyshire.
- Preparation: the activated sludge was washed by repeatedly settling and resuspending with the test water.
- Usage rate: equivalent to 400 mg dry weight/l. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 400 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 24 °C
- Aeration of dilution water: by magnetic stirrers and compressed air via narrow bore glass tubes.
- Continuous darkness: yes
SAMPLING
- Evalporation: losses by evaporat'ion were made good by the addition of deionised water immediately prior to sampling.
- Sampling method: 20 ml samples were withdrawn from each vessel and filtered through 0.5 µm disposable filtres. The first 5 ml was discarted.
CONTROL AND BLANK SYSTEM
- Abiotic control: containing the test material and activated sludge poisoned by the addition of mercuric chloride. - Reference substance:
- diethylene glycol
- Parameter:
- % degradation (DOC removal)
- Value:
- 68
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- rate corrected for abiotic adsorption
- Details on results:
- Preliminary investigational work showed that there was a 44 % loss of test material, as DOC, on addition of a test material stock solution to activated sludge. This suggested that the test material was adsorbed to activated sludge and therefore an abiotic test vessel, containing the test material and activated sludge poisoned by the addition of mercuric chloride, was included in the main study in order to correct percentage degradation values for any adsorption that occurred.
The results obtained during the main study indicated that there was no significant immediate adsorption of the test material in the abiotic test vessel and therefore the initial loss of test material, as DOC, observed in the preliminary work conducted and the main study is considered to be due to rapid biodegradation of the test material by the activated sewage sludge micro-organisms present.
Test item attained 91 % degradation, as loss of DOC, after 28 days. The degradation rate corrected for abiotic adsorption of the test material to the activated sewage sludge was 68 % after 28 days.
No definite guidelines are given for pass criteria of test materials in the OECD Guidelines, however, a result of 68 % degradation could be taken as evidence of inherent biodegradabiIity.
pH values did not drop below 6.7 in any culture vessel during the course of the study and were adjusted to pH 7.4 on each sampling occasion. Dissolved oxygen levels remained at approximately 5.6 mg O2/l or above in all cultures. - Results with reference substance:
- The standard material, Digol, attained 99 % degnadation after 28 days, thereby confirmi g the suitability of the inoculum and culture conditions.
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Inherently biodegradable
- Executive summary:
The biodegradability of test item exposed to a mixed population of activated sewage sludge was investigated under aerobic static conditions. The procedures outlined into the OECD guideline 302 B were followed.
Preliminary investigational work showed that there was a 44 % loss of test material, as DOC, on addition of a test material stock solution to activated sludge. This suggested that the test material was adsorbed to activated sludge and therefore an abiotic test vessel, containing the test material and activated sludge poisoned by the addition of mercuric chloride, was included in the main study in order to correct percentage degradation values for any adsorption that occurred.
The results obtained during the main study indicated that there was no significant immediate adsorption of the test material in the abiotic test vessel and therefore the initial loss of test material, as DOC, observed in the preliminary work conducted and the main study is considered to be due to rapid biodegradation of the test material by the activated sewage sludge micro-organisms present.
Test item attained 91 % degradation, as loss of DOC, after 28 days. The degradation rate corrected for abiotic adsorption of the test material to the activated sewage sludge was 68 % after 28 days.
No definite guidelines are given for pass criteria of test materials in the OECD Guidelines, however, a result of 68 % degradation could be taken as evidence of inherent biodegradabiIity.
pH values did not drop below 6.7 in any culture vessel during the course of the study and were adjusted to pH 7.4 on each sampling occasion. Dissolved oxygen levels remained at approximately 5.6 mg O2/l or above in all cultures.
Conclusion
Inherently biodegradable.
Reference
Overall on test results
Day | DOC* value mg/l (mean of 3 assays) | % degradation | ||||
Test item | Test item - abiotic vessel | Reference substance | Test item | Test item - abiotic vessel | Reference substance | |
0 hr | 77.44 | 99.11 | 100.20 | 23 | 1 | 3 |
3 hrs | 72.69 | 89.27 | 101.20 | 27 | 11 | 2 |
1 | 65.92 | 89.21 | 99.47 | 34 | 11 | 3 |
2 | 67.50 | 85.50 | 88.50 | 33 | 15 | 16 |
3 | 44.00 | 85.00 | 83.00 | 56 | 15 | 19 |
6 | 26.00 | 85.00 | 28.00 | 74 | 15 | 74 |
8 | 23.42 | 80.83 | 9.24 | 77 | 19 | 95 |
10 | 22.40 | 47.34 | 9.69 | 78 | 53** | 93 |
14 | 16.44 | 76.56 | 6.24 | 84 | 23 | 99 |
16 | 14.97 | 76.31 | 6.38 | 85 | 24 | 98 |
21 | 10.04 | 80.15 | 5.34 | 90 | 20 | 99 |
23 | 9.02 | 81.28 | 4.91 | 91 | 19 | 100 |
27 | 12.31 | 84.14 | 6.36 | 90 | 16 | 99 |
28 | 8.89 | 77.10 | 6.05 | 91 | 23 | 99 |
*All values are blank corrected.
** The high % degradation value is considered to be due to sampling and/or analytical variation.
Description of key information
Inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
- Type of water:
- freshwater
Additional information
The biodegradability of test item exposed to a mixed population of activated sewage sludge was investigated under aerobic static conditions, according to the procedures outlined into the OECD guideline 302 B. Preliminary investigational work showed that there was a 44 % loss of test material, as DOC, on addition of a test material stock solution to activated sludge. This suggested that the test material was adsorbed to activated sludge and therefore an abiotic test vessel, containing the test material and activated sludge poisoned by the addition of mercuric chloride, was included in the main study in order to correct percentage degradation values for any adsorption that occurred.
The results obtained during the main study indicated that there was no significant immediate adsorption of the test material in the abiotic test vessel and therefore the initial loss of test material, as DOC, observed in the preliminary work conducted and the main study is considered to be due to rapid biodegradation of the test material by the activated sewage sludge micro-organisms present. Test item attained 91 % degradation, as loss of DOC, after 28 days. The degradation rate corrected for abiotic adsorption of the test material to the activated sewage sludge was 68 % after 28 days.
No definite guidelines are given for pass criteria of test materials in the OECD Guidelines, however, a result of 68 % degradation could be taken as evidence of inherent biodegradabiIity.
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