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EC number: 611-056-6 | CAS number: 538313-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Lack of analytical determination of the substance
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate is incuded in the report
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test mixtures were prepared by adding 16 mL synthetic sewage to the appropriate amounts of test item (directly weighed an incorporated into the test vessel) and water followed by the addition of inoculum to obtain a final volume of 500 mL containing 1.5 g/L of suspended solids and different nominal concentrations of the test item.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The inoculum was prepared from activated sludge sampled the 5 December 2016, in the aeration tank treatment plant receiving predominantly domestic sewage (Montataire - (60) France). After settling, the supernatant of activated sludge was discarded and the sludge re-suspended in chlorine-free tap water. The wash-water was again removed after settling of sludge and activated sludge were re-suspended in chlorine-free tap water.The inoculum was continuously aerated and 50 mL of synthetic sewage feed/L activated sludge were added each day until use.Test carried out 6 December 2016:dry matter of inoculum: 3.9 g/LpH: 7.4192.3 mL of inoculum were added per test flask corresponding to a concentration of suspended solids (SS) in the test mixture of 1.5 g/L
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not available
- Test temperature:
- between 18.5 and 21.5°C
- pH:
- Between 7.1 and 7.7Between 7.3 and 8.0 (test with ATU)
- Salinity:
- Not available
- Conductivity:
- Not available
- Nominal and measured concentrations:
- 1.0 – 10 – 100 and 1000 mg/L (nominal concentrations)
- Details on test conditions:
- - Vessel: 1 litre glass beakers- Inoculum: prepared from activated sludge- Dilution water: deionised water- Medium: synthetic sewage feed- Total volume of mixtures: 500 mL- Air supply: clean, oil-free. Air flow 0.5 to 1 litre/ min.
- Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Remarks:
- Available information has shown that the test item is not soluble in water.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Available information has shown that the test item is not soluble in water.
- Details on results:
- The results show that under the test conditions the test item: CuDABT has no significant toxic effect on respiration of activated sludge at concentrations 1.0 and 10.0 mg/L and approximately 10% of inhibition at 100 mg/L for total respiration after a contact time of 3 hours.Significant inhibitory effects have been observed at the highest concentration tested: 1000 mg/L.No oxygen consumption was observed for abiotic controls during this test, showing a lack of reducing properties of the test item.
- Results with reference substance (positive control):
- 3,5–DCP: 3.13 mg/L: 34.5% of inhibition10.0 mg/L: 48.3% of inhibition32.0 mg/L: 86.2% of inhibitionThe EC50 3,5-DCP, after a contact time of 3 hours, was graphically determined between 10 and 20 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- The blank controls oxygen uptake rate remained above 20 mg O2/g h during the test and the coefficient of variation did not exceed 30% at the end of the test. EC50 (3 hours) of 3,5-dichlorophenol were in the range 2 mg/L and 25 mg/L.
- Conclusions:
- In the test conditions, an EC50 greater than of 1000 mg/L (nominal concentration) has been found for the test item CuDABT, after a contact time of 3 hours.
- Executive summary:
The total respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed is measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item: CuDABT under otherwise identical conditions is also measured. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean respiration rates of the controls.
In the test conditions, no oxygen consumption was observed for abiotic controls during this range-finding test, showing a lack of reducing properties of the test item and an EC50 greater than of 1000 mg/L (nominal concentration) after a contact time of 3 hours.
Reference
Specific inhibitor of nitrification: N-allylthiourea (ATU)
Identification |
Q1 |
Q2 |
Dt |
RH |
RHS |
IH |
pH |
|
T0 |
T3h |
|||||||
Blank control 1 |
6.9 |
5.3 |
4 |
24.0 |
16.0 |
/ |
7.3 |
8.0 |
Blank control 2 |
6.2 |
4.1 |
4 |
31.5 |
21.0 |
7.3 |
8.0 |
|
Blank controls |
Mean |
27.8 |
18.5 |
/ |
||||
Coefficient of variation (%) |
19.1 |
|||||||
CuDABT 1.0 mg/L |
6.1 |
4.4 |
4 |
25.5 |
17.0 |
8.3 |
7.3 |
7.9 |
CuDABT 10.0 mg/L |
6.0 |
4.1 |
4 |
28.5 |
19.0 |
/ |
7.3 |
8.0 |
CuDABT 100 mg/L |
6.1 |
4.1 |
4 |
30.0 |
20.0 |
/ |
7.4 |
7.9 |
CuDABT 1000 mg/L |
6.3 |
5.0 |
4 |
19.5 |
13.0 |
29.9 |
7.3 |
7.9 |
The test series carried out with the N-allylthiourea (ATU) showed that the oxygen uptake rates were lower for the blank controls and for the test item in the presence of ATU compared to in absence of ATU. This difference between the rates therefore indicates that there is an oxygen uptake due to the nitrification. Nevertheless, the inhibition percentages obtained during this study, whether for total respiration or for heterotrophic respiration, are very similar and less than 50% of inhibitory effect for all test concentrations.
Description of key information
In the test conditions, an EC50 greater than of 1000 mg/L (nominal concentration, available information has shown that the test item is not soluble in water) has been found for the test item CuDABT, after a contact time of 3 hours.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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