Dec-9-en-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 July 2002 - 13 August 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dec-9-en-1-ol

EC Number:

235-878-6

EC Name:

Dec-9-en-1-ol

Cas Number:

13019-22-2

Molecular formula:

C10H20O

IUPAC Name:

dec-9-en-1-ol

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): 9-Decenol-1
- Appearance: Clear liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: calculated value: 0.99, 1.70, 2.83, 4.95, 8.48, 14.14 mg/L
The concentration are based on the test item.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
stock solution: the test item was equilibrated in synthetic fresh water (without NaHCO3), stirred and finally filtered off. The filtrate served as stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea Daphnia magna Strauss
- Strain/clone: clone 5
- Source: inhouse
- Feeding during test: none
- Food type: fresh water algae (for breeding)
- Frequency: daily (for breeding)

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Females are separated from the offspring 24 hours before the star of the test. The offspring produced overnight are transferred into test water and are used for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

19.50 - 20.48 °C

pH:

7.5 - 8.1

Dissolved oxygen:

7.6 - 8.3 mg/L

Nominal and measured concentrations:

Nominal concentration: 0, 0.99, 1.70, 2.83, 4.95, 8.48, 14.14 mg/L
Measured concentrations (0h): 1.12, 1.63, 2.96, 4.80, 8.60, 14.44 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: round bottle test tubes
- Type: closed
- Material, size, headspace, fill volume: 10 mL
- Aeration: closed with laboratory film (Parafilm)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.8 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

5 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The highest concentration tested in this experiment at which after 48h exposure no immobilisation (≤ 10%) occured, was: 1.7 mg/L.
The lowest concentration tested in this experiment at which after 48h exposure 100% immobilisation occured, was: 14 mg/L.

Results with reference substance (positive control):

- Results with reference substance valid: yes
- Relevant effect levels: % immobilisation
- Limit test: no
- Influence of the reference substance (potassium dichromate) after 24h:
% of immobilised Daphnia:
- 1.0 mg/L: 35%
- 2.0 mg/L: 100%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48h EC50 = 3.8 mg/L
95% confidence limits: 3.0 mg/L - 4.7 mg/L
24h EC50 = 5.0 mg/L
95% confidence limits: 3.9 mg/L - 6.4 mg/L
The highest concentration tested in this experiment at which after 48h exposure no immobilisation (≤ 10%) occured, was: 1.7 mg/L.
The lowest concentration tested in this experiment at which after 48h exposure 100% immobilisation occured, was: 14 mg/L.