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EC number: 480-060-6 | CAS number: 24065-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 Feb - 26 Feb 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Remarks:
- The test item and reference substance concentrations are not confirmed by analytical methods as they are directly weighed into the test vessels.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct weighing into test flasks
- Controls: Yes, blank control
- Test concentration separation factor: 1.8 - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: mixed population of aquatic microorganisms (activated sludge)
- Name and location of sewage treatment plant where inoculum was collected: aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim)
- Method of cultivation: Stirring period of test item before start of incubation time: 16 h.
- Pretreatment: aeration of the activated sludge, daily fed with synthetic medium.
- Initial biomass concentration: 960 mg/L suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- with permanent aeration
- Test temperature:
- 19.0 to 19.4 °C
- pH:
- 6.2 to 7.7
- Dissolved oxygen:
- 4.7 to 7.9 mg O2/L at the start
4.9 to 7.9 mg O2/ L at the end - Nominal and measured concentrations:
- 560, 1000, 1800, 3200, 5600 mg/L (nominal concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flask
- Aeration: Permanent aeration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2 (blank control), 1 (positive control)
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 960 mg/L suspended solids
- Nutrients provided for bacteria: Daily feed with synthetic medium
EFFECT PARAMETERS MEASURED
- respiration inhibition at 3 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol (Purity; 97%; Batch number: 15809KI-392) 2.5, 5, 10, 20, 40 mg/L (nominal concentration)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 737.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: 32.5 mg/L*h
- Abiotic control: no physico-chemical oxygen consumption was observed at that test substance concentration of 10000 mg/L. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50=9.3 mg/L (confidence limits were 7.9 mg/L and 11.1 mg/L). - Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- The test substance showed 93.8% respiration inhibition of activated sludge at a test item concentration of 5600 mg/L; EC50=737.2 mg/L (calculated concentration).
- Executive summary:
The study was performed to assess the toxicity of the test substance to bacteria. The study was conducted in accordance with the Commission Directive 88/302/EEC Part C, Method 11 (1988). The activated sludge was exposed to the test substance at different concentration (560, 1000, 1800, 3200, 5600 mg/L; nominal concentration). The respiration rate of each mixture was determined after aeration period of 3 hours. The test substance showed 93.8% respiration inhibition of activated sludge at a test item concentration of 5600 mg/L. The following value was determined EC50=737.2 mg/L (calculated concentration).
Reference
Oxygen content, temperature, and pH values during exposure phase
Test concentration | O2 start | O2 end | Time (start-end) | Temp. | pH | |
[mg/L] | [mg O2/L] | [mg O2/L] | [min.] | [°C] | ||
Test item | 560 | 5.6 | 4.9 | 2 | 19.2 | 7.4 |
1000 | 6.3 | 5.8 | 3 | 19.2 | 7.0 | |
1800 | 6.3 | 5.9 | 3 | 19.2 | 7.2 | |
3200 | 7.2 | 7.0 | 4 | 19.3 | 7.0 | |
5600 | 7.4 | 7.2 | 6 | 19.4 | 7.0 | |
control 1 | 5.0 | 2.8 | 4 | 19.0 | 7.7 | |
Control 2 | 4.7 | 3.1 | 3 | 19.4 | 7.7 | |
Physico-chemical oxygen consumption control | 10000 | 7.9 | 7.9 | 7 | 19.2 | 6.2 |
Validity criteria for the measurement of the bacterial toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. If the rate is lower, the test should be repeated with washed activated sludge or with the sludge from another source. The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. | The oxygen uptake rate of the controls were 32.0 and 33.0 mg/L*h (960 mg/L suspended solids). | Yes |
Reference substances In a 2004 international ring test organized by ISO using activated sludge derived from domestic sewage, the EC50 of 3,5-dichlorphenol was found to lie in the range 2 mg/L to 25 mg/L for total respiration, 5 mg/L to 40 mg/L for heterotrophic respiration and 0.1 mg/L to 10 mg/L for nitrification respiration. If the EC50 of 3,5-DCP does not lie in the expected range, the test should be repeated with activated sludge from another source. The EC50 of copper (II) sulphate pentahydrate should lie in the range of 53-155 mg/L for the total respiration. | The EC50 value for the reference substance (3,5-DCP; total respiration) was 9.3 mg/L (confidence limits were 7.9 mg/L and 11.1 mg/L). | Yes |
The test should be performed at a temperature within the range 20±2°C. | During the test the temperature in the test vessels was maintained in the range of 19.0 C° to 19.4 C°. | Yes |
Description of key information
A study was performed to assess the toxicity of the test substance to bacteria. the study was conducted in accordance with the Commission Directive 88/302/EEC Part C, Method 11 (1988). The activated sludge was exposed to the test substance at different concentration (560, 1000, 1800, 3200, 5600 mg/L; nominal concentration). The respiration rate of each mixture was determined after aeration period of 3 hours. The test substance showed 93.8% respiration inhibition of activated sludge at a test item concentration of 5600 mg/L. The following value was determined EC50=737.2 mg/L (calculated concentration).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 737.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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