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EC number: 247-667-6 | CAS number: 26402-22-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Nov - 1 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeschutz Rheinland-Pfalz, Mainz, Germany
Test material
- Reference substance name:
- Esterification product of glycerol and C8-C12 (even numbered) fatty acids
- Molecular formula:
- not applicable, substance is UVCB
- IUPAC Name:
- Esterification product of glycerol and C8-C12 (even numbered) fatty acids
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and all test item concentrations
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The water-accommodated fractions (WAF) of the concentrations to be tested were prepared. This was done by weighing the nominal load, adding the appropriate amount of algal medium (demineralised water enriched with minerals but without algae) and shaking for 24 hours. After membrane filtration (0.45 µm, PTFE-filter), the solutions were used to prepare the treatments.
- Eluate: no
- Differential loading: yes
- Controls: blank control; positive control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): After filtration, the test solutions were clear.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: freshwater green algae
- Strain: SAG 86.81
- Source (laboratory, culture collection): obtained in January 2016 by MBM Sciencebridge GmbH, Institut für Pflanzenphysiologie of Universität Göttingen, Germany
- Method of cultivation: The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.6 – 22.6 °C
- pH:
- Control: 7.8 - 7.9
Treatments: 7.4 - 7.8 - Nominal and measured concentrations:
- nominal: 1 / 3.2 / 10 / 32 / 100 mg/L and control
measured: test item not detectable after 72 h - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks with a total volume of 65 mL; flasks were covered with perforated plastic foil acting as a stopper
- Aeration: constantly shaken
- Initial cells density: 2.8 *10E+03 cells/mL
- Control end cells density: 3.6 *10E+05 (mean) ± 1.6 *10E+03 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, according to guideline
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: pH and temperature measured at test start and end
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous light
- Light intensity: 5000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times. Additionally, the treatments were examined microscopically at the end of the test in order to assess the appearance of the algae and detect abnormalities.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: not reported - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 7.2 - 142 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 1.2 - 15 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CI: 9.5 - 18 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- other: yield
- Remarks on result:
- other: 95% CI: 6.1 - 9.2 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Microscopically, cells appeared normal and healthy in the blank control and the three lowest treatments at the end of the test. No cells were visible at the the two highest concentrations.
- For details on biological results, please refer to section "Any other information on results incl. tables" - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- The 72h-EC50 values of potassium dichromate were determined in a separate reference test. The values are within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
- ErC50 (72 h) = 0.94 mg/L (95% CI: 0.90 - 0.98 mg/L)
- EyC50 (72 h) = 0.42 mg/L (95% CI: 0.39 - 0.45 mg/L)
Any other information on results incl. tables
Table 1: Biological results; cell numbers
Nominal Loading Rate in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
Mean |
2800 |
12050 |
38687 |
360727 |
Blank control |
SD |
0 |
652 |
4455 |
16371 |
1 |
Mean |
2800 |
11140 |
30947 |
338413 |
1 |
SD |
0 |
1512 |
3685 |
19816 |
3.2 |
Mean |
2800 |
11020 |
34640 |
335167 |
3.2 |
SD |
0 |
661 |
1922 |
21650 |
10 |
Mean |
2800 |
11707 |
32113 |
257133 |
10 |
SD |
0 |
439 |
806 |
31787 |
32 |
Mean |
2800 |
6487 |
9693 |
10433 |
32 |
SD |
0 |
948 |
2017 |
2005 |
100 |
Mean |
2800 |
37887 |
38840 |
21660 |
100 |
SD |
0 |
8890 |
6687 |
1735 |
Table 2: Inhibition values for growth rate and yield
Nominal Loading Rate in mg/L |
% Inhibition |
|
Growth Rate (0-72h) |
Yield (0-72h) |
|
Blank control |
0 |
0 |
1 |
1.32 |
6.23 |
3.2 |
1.52 |
7.14 |
10 |
7.06 |
28.94 |
32 |
73.19 |
97.87 |
100 |
57.93 |
94.73 |
Table 3: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
factor of 129 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
34% |
yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
1% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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