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EC number: 605-495-2 | CAS number: 167948-98-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 20, 2002 - January 17, 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch No.: L605517
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Remarks:
- Source: stp Groß-Zimmern, Germany
- Details on inoculum:
- The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 104 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: reconstituted test water according to OECD 301 F
- Additional substrate: no
- Solubilising agent: no
- Test temperature: 21 - 22 °C
- pH: 7.6
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of 500 mL
- Number of culture flasks/concentration: 2
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic, 63231 Neu Isenburg, Germany
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: Evolved carbon dioxide was absorbed in an aqueous solution (45%) of potassium hydroxide
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks (test water, activated sludge)
- Abiotic sterile control: 1 flask (test water, test item)
- Toxicity control: 1 flask (test water, test item, reference item, activated sludge)
- Procedure control: 1 flask (test water, reference item, activated sludge) - Reference substance:
- aniline
- Remarks:
- 25.6 mg/L
- Test performance:
- The oxygen demand of the inoculum control (medium and inoculum) was 7.4 mg O2/L and thus not greater than 60 mg O2/L within 28 days. The pH value of the test item flasks at the end of the test was pH 7.6 – 7.7 and is thus within the range of pH 6.0 – 8.5. The percentage of degradation of the reference item Aniline reached the level for ready biodegradability (about 60 %) within 8 days. The difference of duplicate values for the degradation of the test item at any time during the test was less than 20 %. Therefore all validity criteria for the study are fulfilled.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- -1
- Sampling time:
- 28 d
- Details on results:
- After correction for the mean biochemical oxygen demand of the inoculum controls the percentage of the test item reached -1 % biodegradation after 28 days of incubation.
- Results with reference substance:
- The reference item was sufficiently degraded to a mean of 81 % after 14 days, and to 89 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, 41 % biodegradation was noted within 14 days and 43 % biodegradation was determined after 28 days of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The degradation rate of the test material was -1 % after 28 days and did not reach 60 % within the 10-day window and after 28 days of incubation. The test item can therefore be considered to be not readily biodegradable.
- Executive summary:
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The GLP study was performed according to OECD TG 301F. After correction for the mean biochemical oxygen demand of the inoculum controls the percentage of the test item reached -1 % after 28 days of incubation. The reference item was sufficiently degraded to a mean of 81 % after 14 days, and to 89 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, 41 % biodegradation was noted within 14 days and 43 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. The test item can therefore be considered to be not readily biodegradable.
Reference
Description of key information
The degradation rate of the test material was -1 % after 28 days and did not reach 60 % within the 10-day window and after 28 days of incubation. The test item can therefore be considered to be not readily biodegradable (reference 5.2.1 -1).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The GLP study was performed according to OECD TG 301F. After correction for the mean biochemical oxygen demand of the inoculum controls the percentage of the test item reached -1% after 28 days of incubation. The reference item was sufficiently degraded to a mean of 81% after 14 days, and to 89% after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, 41% biodegradation was noted within 14 days and 43% biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. The test item can therefore be considered to be not readily biodegradable.
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