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EC number: 417-440-8 | CAS number: 2516-92-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted April 4, 1984,
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,10-bis(2,2,6,6-tetramethyl-1-piperidinyloxy)-1,10-dioxodecane
- EC Number:
- 417-440-8
- EC Name:
- 1,10-bis(2,2,6,6-tetramethyl-1-piperidinyloxy)-1,10-dioxodecane
- Cas Number:
- 2516-92-9
- Molecular formula:
- C28 H50 N2 O6
- IUPAC Name:
- bis(1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl) decanedioate
- Details on test material:
- Identification code (lab) = TKA 40075
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
During the final test samples for analysis were taken from the control, the additive-control, the filtered and unfiltered solution of 100 mg/l.
- Sampling method:
Frequency: at t= 0h and t=48h.
Volume: 10 ml from the approximate centre of the test vessel.
- Sample storage conditions before analysis:
Samples taken at t=0h were stored in a deep- freeze until analysis. Samples taken at t=48h were analyzed on the day of sampling.
Test solutions
- Vehicle:
- yes
- Remarks:
- Cremophor EL as a dispersing agent.
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Tests solutions of 100 mg/l were prepared by adding quantitatively 29.5 (A: filtrate) or 29.3 mg (B: supersaturated solution) of test substance to 300 ml ISO-medium.
Since the solubility of the test substance in water was limited, Cremophor EL was used for the preparation of stock solutions. The final concentration of Cremophor EL in the test solutions was 100 mg/l.
The solutions were stirred for 94 hours. Subsequently, solution A was filtered through a paper filter and as such tested, i.e. 2 x 80 ml divided over 2 vessels. From solution B 2 x 80 ml were taken and tested without filtration.
Both test solutions were turbid. Undissolved test substance particles were observed in the supersaturated solution (B).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: common water flea
- Strain/clone: not specified
- Source: laboratory culture
- Age of parental stock : maximum 4 weeks
- Feeding during test:
Daily, a volume of 0.25 ml of a Chlorella pyrenoidosa suspension with a cell density of 2*10^8 cells/ml was added to each vessel containing ca. 50 ml of test solution as feed for the daphnids i.e. a ration per organism of about 50*10^6 cells/day (corresponding with ca. 0.5 mg C/Daphnia/day).
ACCLIMATION
not specified
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.0 to 21.0°C
- pH:
- 8.1-8.2
- Dissolved oxygen:
- 9.3-9.5 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Aeration:
No aeration of the test solution, except when the presence of test substance induces oxygen depletion.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
ISO, prepared in milli-RO water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobility
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
No range-finding test was performed. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 15.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In the control, no daphnia became immobilized or trapped at the surface of the water. The analytical program provided clear evidence that the actual test concentration had been maintained at 80% of the initial concentration in the filtered solution. The unfiltered solution appeared to be an unstable dispersion.
Further, all test conditions remained within the ranges prescribed by the protocol.
Some effects were observed with the unfiltered 100 mg/L solution, which had undissolved test substance particle deposits on the bottom of the test vessel. These effects were probably related with the presence of larger test substance particles, which were not present in the filtered solution. - Results with reference substance (positive control):
- The actual responses in this reference test are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at NOTOX.
The 24h-EC50 was 0.92 mg/l with 95 % fiducial limits of 0.83 and 1.07 mg/l. The 48h-EC50 of this test corresponded with 0.75 mg/l, i.e. the geometric mean between 0.56 (0 %) and 1.0 mg/l (100 %).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, the 48h-EC50 of TKA 40075 (GU 10-415) for immobilization of daphnia was greater than the maximum attainable concentration of TKA 40075 (GU 10-415) in test medium (ca. 16 mg/l).
- Executive summary:
Acute Toxicity Study in Daphnia magna with TKA 40075 (GU 10-415).
The study procedures described in this report were based on the EEC directive 92/69, Part C.2. "Acute toxicity for Daphnia" and the OECD guideline No. 202: "Daphnia sp., Acute Immobilisation Test", Adopted April 4, 1984,
The final test was a limit study exposing daphnia for a maximum of 48 hours to both a supersaturated solution of nominally 100 mg/l and its filtrate. The test solutions consisted of dispersions prepared with the use of Cremophor EL as a dispersing agent. These dispersions were stirred for ca. 94 hours before the start of the exposure. Subsequently, one of these dispersions was filtered through a paper filter and as such tested while the other was tested without filtration. Both were turbid while the unfiltered dispersion contained undissolved test substance particles dispersed through the medium. The test was performed in duplicate with 10 daphnia per vessel.
Analysis of the samples taken at the start (t=0) of the test showed that the exposure concentration in the unfiltered solution of nominal 100 mg/l was 82.3 mg/l, while a concentration of 16.6 mg/l was measured in the filtrate.
At the end of the exposure period, the measured concentrations had not decreased significantly in the filtrate. However, in the unfiltered solution the measured concentration had decreased to 14.7 mg/l.
This was most likely caused by deposits of undissolved test substance particles on the bottom of the test vessel.Under the conditions of the present study, the 48h-EC50 of TKA 40075 (GU 10-415) for immobilization of daphnia was greater than ca. 16 mg/l, a supersaturated concentration of TKA 40075 (GU 10-415) in test medium.
The effects observed in daphnia exposed to the unfiltered solution of nominally 100 mg/l were probably related with the presence of larger test substance particles, which were not present in the filtered solution.
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