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EC number: 257-885-3 | CAS number: 52372-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental rsults of read across chemicals
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE report is prepared based on short term toxicity to aquatic algae study.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
- Common name: Solvent Red 197
- Molecular formula:C23H19N5O
- Molecular weight: 381.437 g/mol
- Smiles : c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
- InChI : 1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
- Substance Type: Organic - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- 1. The stock solution 100 mg/l was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
2. The stock solution (100 mg/L) was prepared by dissolving pinkish red powder in OECD growth medium. The stock solution was kept in ultrasonic bath for 20min Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- 1 study:
Test at highest concentration: 7.9 (Change to 7.4 during tests)
Control: 8.1 (changed to 7.7 during test)
Control + acetone: 8.1 change to 7.7
2. WIthout adjustment
The sample at concentration 40 mg/l: pH=8 changed to 7.9 during the test
Control: pH= 8.1 changed to pH= 7.5 during the test - Nominal and measured concentrations:
- 1. 0, 0, 6, 12, 24, 50 and 100 mg/l
2. 0, 2.5, 5, 10, 20 and 40 mg/L - Details on test conditions:
- 1. TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 17.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: In ist study: 95 % CI was 14.2 - 21.6 mg/l
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 13.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: In 2nd study: 95 % CI was 11.9 - 15.2 mg/l
- Results with reference substance (positive control):
- Results with reference substance valid
1. - EC50: 0.69 mg/L
2. - EC50: 0.75 mg/L - Reported statistics and error estimates:
- ErC50 was calculated using non-linear regression by the software Prism 4.0
- Validity criteria fulfilled:
- yes
- Conclusions:
- 1. Based on the growth rate inhibition of Desmodesmus subspicatus, by the test chemical after the exposure for 72 hrs, the ErC50 value was determine to be 17.5 mg/l.
2. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study. - Executive summary:
Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic algae is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:
Toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the growth of aquatic algae and cyanobacteria. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution 100 mg/l was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 0, 6, 12, 24 and 100 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 17.5 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the ErC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution (100 mg/L) was prepared by dissolving pinkish red powder in OECD growth medium. The stock solution was kept in ultrasonic bath for 20min Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 2.5, 5, 10, 20 and 40 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and cyanobacteria and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Reference
Description of key information
1. Based on the growth rate inhibition of Desmodesmus subspicatus, by the test chemical after the exposure for 72 hrs, the ErC50 value was determine to be 17.5 mg/l.
2. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study.
Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic as value ranges from 13.4 -17.5 mg/l and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 17.5 mg/L
Additional information
Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic algae is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:
Toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the growth of aquatic algae and cyanobacteria. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution 100 mg/l was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 0, 6, 12, 24 and 100 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 17.5 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the ErC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution (100 mg/L) was prepared by dissolving pinkish red powder in OECD growth medium. The stock solution was kept in ultrasonic bath for 20min Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 2.5, 5, 10, 20 and 40 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and cyanobacteria and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
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