Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-588-5 | CAS number: 4420-74-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-09-01 to 2000-10-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-trimethoxysilylpropane-1-thiol
- EC Number:
- 224-588-5
- EC Name:
- 3-trimethoxysilylpropane-1-thiol
- Cas Number:
- 4420-74-0
- Molecular formula:
- C6H16O3SSi
- IUPAC Name:
- 3-trimethoxysilylpropane-1-thiol
Constituent 1
Method
- Target gene:
- histidine operon (Salmonella strains)
tryptophan operon (E. coli strain)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- preliminary assay: 5000 µg/plate
75-5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: compatibility with the target cells
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- all strains, + MA, 1.0 µg/plate (Salmonella) 10 µg/plate (E. coli)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 98, - MA, 1.0 µg/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA 100 TA 1535, - MA, 1.0 µg/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537, - MA, 75 µg/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- WP2 uvrA, - MA, 1000 µg/plate
- Details on test system and experimental conditions:
- ACTIVATION:
S9 mix:
- 10% S9
- 5 mM glucose-6-phosphate
- 4 mM ß-nicotinamide-adenine dinucleotide phosphate
- 8 mM MgCl2
- 33 mM KCl
- 100 mM phosphate buffer (pH 7.4)
METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 60±2 min at 37±2°C
- Exposure duration: 48-72 hours at 37±2°C
SELECTION AGENT (mutation assays): minimal agar
NUMBER OF REPLICATIONS: triplicate plates; 2 independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: other: condition of bacterial background lawn - Evaluation criteria:
- For the test article to be evaluated positive, it must cause a dose-related increase in the mean revertants per plate of at least one tester strain over a minimum of two increasing concentrations of test article.
Data sets for TA 1535 and TA 1537 were judged positive if the increase in mean revertants is ≥ 3x the mean vehicle control value.
Data sets for TA 98, TA 100 and WP2 uvrA were judged positive if the increase in mean revertants is ≥ 2x the mean vehicle control value.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic potential
Any other information on results incl. tables
Preliminary toxicity assay: number of revertants per plate
Concentration (µg/plate) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|||||
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
|
Vehicle |
22 |
12 |
128 |
118 |
18 |
14 |
7 |
5 |
15 |
1 |
6.7 |
25 |
14 |
125 |
128 |
22 |
17 |
4 |
4 |
11 |
1 |
10 |
19 |
10 |
103 |
112 |
23 |
13 |
5 |
3 |
19 |
1 |
33 |
24 |
16 |
113 |
110 |
17 |
14 |
4 |
3 |
17 |
1 |
67 |
22 |
19 |
93 |
C |
18 |
11 |
5 |
4 |
14 |
1 |
100 |
23 |
17 |
132 |
111 |
21 |
21 |
4 |
3 |
12 |
1 |
333 |
21 |
11 |
99 |
100 |
18 |
18 |
4 |
4 |
10 |
1 |
667 |
26 |
11 |
111 |
102 |
12 |
16 |
5 |
5 |
16 |
1 |
1000 |
25 |
16 |
99 |
116 |
19 |
17 |
4 |
4 |
14 |
1 |
3333 |
21 |
10* |
98 |
81* |
17 |
10* |
6 |
2* |
22 |
1 |
5000 |
18* |
7** |
64** |
0*** |
14* |
0*** |
2*** |
0*** |
13* |
14** |
* = background lawn slightly reduced
** = background lawn severely reduced
*** = background lawn extremely reduced
C = contaminated
Experiment B1: Number of revertants per plate (mean of 3 plates)
Concentration (µg/plate) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|||||
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
|
Vehicle |
20 |
21 |
92 |
116 |
12 |
9 |
5 |
7 |
10 |
12 |
100 |
19 |
26 |
99 |
115 |
- |
13 |
- |
7 |
13 |
10 |
105 |
- |
- |
- |
- |
10 |
- |
6 |
- |
- |
- |
333 |
22 |
29 |
87 |
119 |
- |
10 |
- |
5 |
14 |
12 |
348 |
- |
- |
- |
- |
12 |
- |
6 |
- |
- |
- |
1000 |
25 |
26 |
93 |
95 |
- |
10 |
- |
6 |
10 |
11 |
1045 |
- |
- |
- |
- |
10 |
- |
4 |
- |
- |
- |
3333 |
18 |
25 |
63** |
79* |
- |
7** |
- |
4* |
11 |
10 |
3485 |
- |
- |
- |
- |
9* |
- |
3** |
- |
- |
- |
5000 |
1*** |
7** |
4*** |
60** |
0*** |
1*** |
0**** |
0*** |
10* |
10 |
Positive control |
567 |
608 |
408 |
488 |
231 |
35 |
580 |
40 |
368 |
162 |
* = background lawn slightly reduced
** = background lawn severely reduced
*** = background lawn extremely reduced
**** = background lawn absent
Experiment B2: Number of revertants per plate (mean of 3 plates)
Concentration (µg/plate) |
TA 98 |
TA 100 |
TA 1535 |
TA 1537 |
WP2 uvrA |
|||||
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
- MA |
+ MA |
|
Vehicle |
13 |
13 |
85 |
97 |
6 |
8 |
4 |
8 |
11 |
10 |
75 |
13 |
15 |
86 |
108 |
9 |
7 |
3 |
4 |
10 |
12 |
200 |
15 |
19 |
89 |
123 |
9 |
7 |
3 |
5 |
11 |
15 |
600 |
12 |
16 |
98 |
85 |
10 |
7 |
4 |
4 |
11 |
10 |
1800 |
10* |
16 |
78* |
100 |
3** |
9 |
3* |
4 |
9* |
11 |
5000 |
0*** |
0*** |
0*** |
0*** |
0*** |
8** |
0*** |
1*** |
7** |
10* |
Positive control |
308 |
258 |
311 |
385 |
94 |
39 |
218 |
45 |
266 |
57 |
* = background lawn slightly reduced
** = background lawn severely reduced
*** = background lawn extremely reduced
Applicant's summary and conclusion
- Conclusions:
- 3-Trimethoxysilylpropane-1-thiol has been tested in a valid bacterial reverse mutation assay, according to the OECD TG 471 (1989), and under GLP, using Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 or E.coli WP2 uvrA.
No increase in the number of revertants was observed in any test strain, with or without metabolic activation. Appropriate positive and solvent controls were added and gave expected results. It is concluded that the test substance is negative for mutagenicity to bacteria under the conditions of the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.