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EC number: 269-102-2 | CAS number: 68187-50-8 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 77498.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation: The test item showed no skin irritation potential in the EpiDerm™in vitro skin irritation test.
Eye irritation: The test item showed no eye irritation potential in the EpiOcular™in vitro eye irritation test.
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation
The potential of the test substance to cause dermal corrosion/irritation was assessed by a single topical application of ca. 25 µL bulk volume (about 17 mg) of the undiluted test substance to a reconstructed three-dimensional human epidermis model in vitro according OECD TG 439.
The irritation test was performed with three EpiDerm™ tissues which were incubated with the test substance for 1 hour followed by a 42-hour post-incubation period. Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation by using a colorimetric test. The reduction of mitochondrial dehydrogenase activity measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the test substance treated epidermal tissues is compared to that of negative control tissues. The quotient of the values indicates the relative tissue viability.
Due to the color of the test substance, it could not be determined whether the test substance can directly reduce MTT. Therefore, an additional MTT reduction control (freeze-killed control tissues (KC)) was introduced. In a pretest, it was demonstrated that the color of the test substance did not interfere with the colorimetric test. The final mean viability of the tissues treated with the test substance determined after an exposure period of 1 hour with an about 42-hour post-incubation was 76.7%. Minimal compound residues remained on the tissues treated with the test substance after the washing procedure.
Based on the results the test item does not show a skin irritation potential in the EpiDerm™in vitro skin irritation test under the conditions chosen (BASF SE, 2017).
Eye irritation
The potential of the test substance to cause ocular irritation was assessed by a single topical application of ca. 50 µL bulk volume (about 21 mg) of the undiluted test substance according to OECD TG 492.
Two test runs were performed. Two reconstructed three-dimensional, human cornea model EpiOcular™ tissues per test run were incubated with the test substance for 6 hours followed by an 18-hour post-incubation period.
Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation by using a colorimetric test. The reduction of mitochondrial dehydrogenase activity measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the epidermal tissues treated with the test substance is compared to that of negative control tissues. The ratio of the values indicates the relative tissue viability.
Due to the color of the test substance, it could not be determined whether the test substance is able to directly reduce MTT. Therefore, an additional MTT reduction control (freeze-killed control tissues (KC)) was introduced. In a pretest, it was demonstrated that the color of the test substance did not interfere with the colorimetric test.
In the 1st test run non-concordant replicate measurements of the test-substance treated tissues was obtained (values for single tissues (without KC correction): 78.4% and 15.6%). The high inter-tissue variability of the test substance occurred because tissue 2 (viable tissue and KC tissue) was mechanically damaged during the washing procedure.
In the 2nd test run the final mean viability of the tissues treated with the test substance was 78.7% (values for single tissues (without KC correction): 68.9% and 89.3%).The value for inter-tissue variability of the test substance is 20.3% and therefore minimal out of the acceptance range. Since all other quality criteria of the test were met and the viability values of both tissues are well above the cut off for eye irritation this deviation is not considered to adversely affect the result of this study.
In both test runs slight to moderate compound residues remained on the test-substance treated tissues after the washing procedure and these tissues were black discolored.
Based on the results the test item does not show an eye irritation potential in the EpiOcular™ in vitro eye irritation test under the conditions chosen (BASF SE, 2017).
Justification for classification or non-classification
Classification, Labeling, and Packaging Regulation (EC) No 1272/2008
The available experimental test data are reliable and suitable for the purpose of classification under Regulation (EC) No 1272/2008. Based on these information the test item is not considered to be classified for skin and eye irritation under Regulation (EC) No 1272/2008, as amended for the tenth time in Regulation (EU) No 2017/776.
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